A Method for Thiarubrine Canals Extraction in Optical Coherence Tomography Images of Schkuhria Pinnata Roots

2014 ◽  
Vol 19 (2-3) ◽  
pp. 151-160 ◽  
Author(s):  
Joanna Sekulska-Nalewajko ◽  
Jarosław Gocławski ◽  
Marzena Wielanek ◽  
Ewa Gajewska ◽  
Szymon Tamborski
Keyword(s):  
Optical Coherence Tomography ◽  
Experimental System ◽  
Region Growing ◽  
Root Culture ◽  
South American ◽  
Optical Coherence ◽  
Central Wavelength ◽  
Custom Made ◽  
System Operating ◽  
Transformed Root Culture

Abstract This paper presents a method of automatic recognition of thiarubrine canals in images obtained with Optical Coherence Tomography technique. The plant material was the Ri-transformed root culture of South American herb Schkuhria pinnata. The series of highresolution OCT B-scans for the study were collected using custom made experimental system operating light of 800 nm central wavelength. The method reduces significant artefacts and uses region growing approach adapted to specific features of OCT images. Results of the identification have been compared with data obtained by specialist for selected B-scans. The algorithm accuracy was also verified using a simple numeric phantom.

scholarly journals Design and Implementation Guidelines for a Modular Spectral-Domain Optical Coherence Tomography Scanner

2018 ◽  
Vol 2018 ◽  
pp. 1-22 ◽  
Author(s):  
Farid Atry ◽  
Israel Jacob De La Rosa ◽  
Kevin R. Rarick ◽  
Ramin Pashaie
Keyword(s):  
Optical Coherence Tomography ◽  
Imaging System ◽  
Optical Design ◽  
Spectral Domain ◽  
Design Parameters ◽  
Optical Coherence ◽  
Custom Made ◽  
Essential Knowledge ◽  
Sd Oct

In the past decades, spectral-domain optical coherence tomography (SD-OCT) has transformed into a widely popular imaging technology which is used in many research and clinical applications. Despite such fast growth in the field, the technology has not been readily accessible to many research laboratories either due to the cost or inflexibility of the commercially available systems or due to the lack of essential knowledge in the field of optics to develop custom-made scanners that suit specific applications. This paper aims to provide a detailed discussion on the design and development process of a typical SD-OCT scanner. The effects of multiple design parameters, for the main optical and optomechanical components, on the overall performance of the imaging system are analyzed and discussions are provided to serve as a guideline for the development of a custom SD-OCT system. While this article can be generalized for different applications, we will demonstrate the design of a SD-OCT system and representative results for in vivo brain imaging. We explain procedures to measure the axial and transversal resolutions and field of view of the system and to understand the discrepancies between the experimental and theoretical values. The specific aim of this piece is to facilitate the process of constructing custom-made SD-OCT scanners for research groups with minimum understanding of concepts in optical design and medical imaging.

scholarly journals Measurement of lung tissue dynamics in artificially ventilated rats with optical coherence tomography

2017 ◽  
Vol 3 (2) ◽  
pp. 79-81
Author(s):  
Christian Schnabel ◽  
Maria Gaertner ◽  
Edmund Koch
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Lung Tissue ◽  
Intravital Microscopy ◽  
Artificial Ventilation ◽  
Imaging Techniques ◽  
Animal Experiments ◽  
Optical Coherence ◽  
Custom Made ◽  
Alveolar Tissue

AbstractDiseases of lung tissue and the airways become a major task for medical care and health care systems in modern industrial countries in the future. Suitable treatment methods and strategies for lung support and artificial ventilation are of dare need. Besides the obvious importance as life-saving intervention, the effects of usually used over-pressure ventilation onto the sensitive alveolar tissue are insufficiently understood. Therefore, it is of great interest to characterize lung tissue during artificial ventilation at the alveolar level. Those measurements can be used to link micromechanics of alveolar structures to mechanical properties of the whole lung like compliance and resistance measured at the ventilator device. This can be done only in animal experiments due to the fact that imaging techniques used in human diagnostics like CT or MRT fail to resolve alveolar tissue structures. The disadvantage of high-resolution techniques like optical coherence tomography (OCT) or intravital microscopy (IVM) is the need of a surgical access to the lung due to the limitation in penetration depth of these techniques. Furthermore, imaging dynamic processes with high-resolution imaging techniques during uninterrupted artificial ventilation is a challenging task. In this study, we present a measurement setup for combined imaging of conventional pressure-controlled ventilated rats and the visualization of volume changes of alveolar structures during one cycle of breath. A custom-made OCT system in combination with a triggered scanning algorithm was used to acquire time-resolved 3D OCT image data. Furthermore, this system was combined with a self-adapting autofocus function for intravital microscopy to track the lung surface keeping the tissue in focal plane. The combination of new dynamic measurement modes for OCT and IVM allows new insights into alveolar tissue and will promote the understanding of mechanical behavior during artificial ventilation.

scholarly journals Optical Coherence Tomography for Examination of Parchment Degradation

2006 ◽  
Vol 2006 ◽  
pp. 1-6 ◽  
Author(s):  
Michalina Góra ◽  
Michael Pircher ◽  
Erich Götzinger ◽  
Tomasz Bajraszewski ◽  
Matija Strlic ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Axis ◽  
Incident Light ◽  
Infrared Light ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Central Wavelength ◽  
Non Invasive ◽  
Spectral Oct ◽  
Iron Gall

A novel application of Optical Coherence Tomography utilizing infrared light of 830 nm central wavelength for non invasive examination of the structure of parchment, some covered with iron gall ink, is presented. It is shown that both the parchment and the ink applied are sufficiently transparent to light of this wavelength. In the study, Spectral OCT (SOCT) as well as Polarisation Sensitive OCT (PS-OCT) techniques were used to obtain cross-sectional images of samples of parchment based on scattering properties. The second technique was additionally employed to recover the birefringence properties and the optical axis orientations of the sample. It was shown that freshly produced parchment exhibits a degree of birefringence. However, this property declines with ageing, and samples of old parchment completely depolarise the incident light.

scholarly journals Comparison of phase-resolved Doppler optical coherence tomography and optical coherence tomography angiography for measuring retinal blood vessels size

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Zohreh Hosseinaee ◽  
Bingyao Tan ◽  
Kostadinka Bizheva
Keyword(s):  
Optical Coherence Tomography ◽  
Blood Vessels ◽  
Optical Coherence ◽  
Acquisition Rate ◽  
Doppler Oct ◽  
Retinal Blood Vessels ◽  
Significant Difference ◽  
Arteries And Veins ◽  
System Operating ◽  
Sd Oct

The goal of this study was to compare two OCT-based methods for measuring retinal blood vessels size: Phase-resolved Doppler OCT (DOCT) and OCT angiography (OCTA). The study was conducted in rats (n= 6) using a SD-OCT system operating at 1060 nm with 92 kHz image acquisition rate. Arteries and veins were separated by the phase polarity. Results from this study showed that the venal diameters are significantly larger than the arterial diameters, and there is no significant difference in the vessel diameters measured by both methods.

scholarly journals Granular Cell Tumor Imaging Using Optical Coherence Tomography

2018 ◽  
Vol 9 ◽  
pp. 117959721879025 ◽  
Author(s):  
David Tes ◽  
Ahmed Aber ◽  
Mohsin Zafar ◽  
Luke Horton ◽  
Audrey Fotouhi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Granular Cell Tumor ◽  
Tumor Imaging ◽  
Granular Cell ◽  
Cell Tumor ◽  
The Body ◽  
Optical Coherence ◽  
Healthy Skin ◽  
Swept Source ◽  
Central Wavelength

Background: Granular cell tumor (GCT) is a relatively uncommon tumor that may affect the skin. The tumor can develop anywhere on the body, although it is predominately seen in oral cavities and in the head and neck regions. Here, we present the results of optical coherence tomography (OCT) imaging of a large GCT located on the abdomen of a patient. We also present an analytical method to differentiate between healthy tissue and GCT tissues. Materials and methods: A multibeam, Fourier domain, swept source OCT was used for imaging. The OCT had a central wavelength of 1305 ± 15 nm and lateral and axial resolutions of 7.5 and 10 µm, respectively. Qualitative and quantitative analyses of the tumor and healthy skin are reported. Results: Abrupt changes in architectures of the dermal and epidermal layers in the GCT lesion were observed. These architectural changes were not observed in healthy skin. Discussion: To quantitatively differentiate healthy skin from tumor regions, an optical attenuation coefficient analysis based on single-scattering formulation was performed. The methodology introduced here could have the capability to delineate boundaries of a tumor prior to surgical excision.

scholarly journals Attenuation coefficient as a quantitative parameter for analyzing cataracts with optical coherence tomography

2020 ◽  
Vol 238 ◽  
pp. 04004
Author(s):  
Pablo Eugui ◽  
J. Harper Danielle ◽  
Johanna Gesperger ◽  
Antonia Lichtenegger ◽  
Conrad W. Merkle ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Attenuation Coefficient ◽  
Optical Coherence ◽  
Quantitative Parameter ◽  
Swept Source ◽  
Tomography System ◽  
Custom Made ◽  
Significant Difference ◽  
Lens Opacities

Crystalline lenses of mice were imaged in vivo with a custom-made swept-source optical coherence tomography system. The use of the attenuation coefficient as a quantitative parameter for investigating the lens opacities magnitude is proposed, demonstrating a significant difference between the values retrieved from cataractous and normal mouse lenses.

scholarly journals Artefact-removal algorithms for Fourier domain quantum optical coherence tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sylwia M. Kolenderska ◽  
Maciej Szkulmowski
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Information ◽  
Experimental System ◽  
Theoretical Background ◽  
Fourier Domain ◽  
Optical Coherence ◽  
Joint Spectrum ◽  
Spectrum Detection ◽  
Artefact Removal ◽  
Quantum Optical

AbstractQuantum Optical Coherence Tomography (Q-OCT) is a non-classical equivalent of Optical Coherence Tomography and is able to provide a twofold axial resolution increase and immunity to resolution-degrading dispersion. The main drawback of Q-OCT are artefacts which are additional elements that clutter an A-scan and lead to a complete loss of structural information for multilayered objects. Whereas there are very practical and successful methods for artefact removal in Time-domain Q-OCT, no such scheme has been devised for Fourier-domain Q-OCT (Fd-Q-OCT), although the latter modality—through joint spectrum detection—outputs a lot of useful information on both the system and the imaged object. Here, we propose two algorithms which process a Fd-Q-OCT joint spectrum into an artefact-free A-scan. We present the theoretical background of these algorithms and show their performance on computer-generated data. The limitations of both algorithms with regards to the experimental system and the imaged object are discussed.

Ultrahigh-resolution optical coherence tomography at 1.3μm central wavelength by using a supercontinuum source pumped by noise-like pulses

2015 ◽  
Vol 13 (2) ◽  
pp. 025101 ◽  
Author(s):  
Yi-Jing You ◽  
Chengming Wang ◽  
Yi-Lun Lin ◽  
Alexey Zaytsev ◽  
Ping Xue ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Ultrahigh Resolution ◽  
Central Wavelength
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