Characterization of phenolic compounds from inner bark of Betula pendula

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaana Liimatainen ◽  
Maarit Karonen ◽  
Jari Sinkkonen ◽  
Marjo Helander ◽  
Juha-Pekka Salminen
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Betula Pendula ◽  
Silver Birch ◽  
Biologically Active ◽  
Array Detector ◽  
Ionization Mass ◽  
Inner Bark ◽  
First Time

Abstract A method has been developed for the characterization of biologically active silver birch (Betula pendula) inner bark phenolics based on high-performance liquid chromatography/diode array detector (HPLC-DAD)/electrospray ionization-mass spectrometry (ESI-MS). It was demonstrated that the inner bark contains high amounts of flavonoids, arylbutanoids, diarylheptanoids, simple phenolic compounds, phenolic acids, lignans, and procyanidins. Altogether, 30 individual compounds were characterized based on their ultraviolet (UV) and MS data. Structures of 22 compounds were confirmed by nuclear magnetic resonance (NMR) spectroscopy. In addition to previously reported phenolic compounds, 12 compounds were identified in silver birch inner bark for the first time; two of them are novel compounds: 3-β-glucopyranosyloxy-2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-propan-1-one and 1,7-bis-(4-hydroxyphenyl)-3-heptanol 3-O-β-ap-iofuranosyl-(1→2)-β-glucopyranoside.

scholarly journals RP-HPLC-DAD-MSn Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Jósquia S Barbosa ◽  
Verônica M Almeida ◽  
Rosilene M Marçal ◽  
Alexsandro Branco
Keyword(s):  
High Performance ◽  
Methanol Extract ◽  
Array Detector ◽  
Ionization Mass ◽  
Diode Array Detector ◽  
Rp Hplc ◽  
Dependent Form ◽  
Ellman’S Method ◽  
First Time

High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3- O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.

scholarly journals Extraction and Characterization of Phenolic Compounds from Rose Hip (Rosa canina L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry

2015 ◽  
Vol 43 (2) ◽  
pp. 349-354 ◽  
Author(s):  
Andreea STĂNILĂ ◽  
Zorița DIACONEASA ◽  
Ioana ROMAN ◽  
Nicușor SIMA ◽  
Dănuț MĂNIUȚIU ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Electrospray Ionization ◽  
Phenolic Acids ◽  
High Performance ◽  
Chemical Characterization ◽  
Ionization Mass ◽  
Rosa Canina ◽  
Rose Hip

Wild berry are a rich of natural compounds which provide them high antioxidant potential. The compounds which provide them these proprieties are known to be vitamins, flavonoids, anthocyanins and phenolic acids. The aim of this study was to extract and characterize bioactive compounds from rose hip (Rosa canina L.) currently found in Romania. A qualitative high-performance liquid chromatography coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive ion mode has been used to identify phenolic compounds from rose hip crude extract. The chromatograms revealed the presence of a large number of compounds (19), identified and grouped as phenolic acids and flavones/ols, flavan-3-ols and also anthocyanins. Based on obtained results these berries can be highly recommended as part of our diet. Also this finding represents a contribution to the chemical characterization of phenolic profile of rose hip.

scholarly journals THE COMPOSITION AND CONTENT OF PHENOLIC COMPOUNDS IN THE LEAVES OF A POTENTIALLY INVA-SIVE SPECIES PHYSOCARPUS OPULIFOLIUS (L.) MAXIM. AND ITS ORNAMENTAL CULTIVARS

2020 ◽  
pp. 149-155
Author(s):  
Anastasiya Petrovna Belanova ◽  
Vera Andreyevna Kostikova ◽  
Galina Ivanovna Vysochina
Keyword(s):  
Invasive Species ◽  
Phenolic Compounds ◽  
High Performance ◽  
Global Scale ◽  
Biologically Active ◽  
Alien Plant ◽  
Resource Potential ◽  
Phenolic Profiles ◽  
Physocarpus Opulifolius ◽  
First Time

The expansion of alien plant species in natural communities is now taking a global scale. Since most invasive plants have economically valuable properties, many researchers agree that these plants must be used as new sources of biological resources. The composition and content of biologically active compounds were studied in the leaves of potentially invasive species of Novosibirsk – Physocarpus opulifolius (L.) Maxim. and its ornamental cultivars Ph. opulifolius ‘Diabolo’, Ph. opulifolius ‘Luteus’ by the method of High-Performance Liquid Chromatography. For the first time an analysis of phenolic profiles of introduced, self-sown and cultivated Ph. opulifolius plants has been performed. Presence of 22 phenolic compounds represented by several groups of substances with predominating flavanols (hyperoside, rutin, astragalin and quercetin) has been established. Avicularin has been only revealed in the leaves of cultivars. The sum of identified flavonols greatly varies in different samples, the highest content is in the cultivar Ph. opulifolius ‘Diabolo’ (14.68 mg/g) and self-sown plants of Ph. opulifolius (14.22 mg/g). The content of the sum of identified flavons (vitexin, isovitexin, baicalin) and phenolic acid (сhlorogenic and caffeic acids) is almost the same in all Ph. opulifolius samples under study. The data obtained will allow to widen the idea of the resource potential of the aggressive invasive species and its ornamental cultivars in the secondary areal.

scholarly journals The Analysis of Phenolic Compounds in Walnut Husk and Pellicle by UPLC-Q-Orbitrap HRMS and HPLC

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3013
Author(s):  
Fang Sheng ◽  
Bangyan Hu ◽  
Qiang Jin ◽  
Jiangbo Wang ◽  
Cuiyun Wu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Development Stage ◽  
Walnut Husk ◽  
First Time ◽  
Comprehensive Characterization ◽  
Product Industry

Husk and pellicle as the agri-food waste in the walnut-product industry are in soaring demand because of their rich polyphenol content. This study investigated the differential compounds related to walnut polyphenol between husk and pellicle during fruit development stage. By using ultra-high performance liquid chromatography-quadrupole-orbitrap (UHPLC-Q-Orbitrap), a total of 110 bioactive components, including hydrolysable tannins, flavonoids, phenolic acids and quinones, were tentatively identified, 33 of which were different between husk and pellicle. The trend of dynamic content of 16 polyphenols was clarified during walnut development stage by high-performance liquid chromatography (HPLC). This is the first time to comprehensive identification of phenolic compounds in walnut husk and pellicle, and our results indicated that the pellicle is a rich resource of polyphenols. The dynamic trend of some polyphenols was consistent with total phenols. The comprehensive characterization of walnut polyphenol and quantification of main phenolic compounds will be beneficial for understanding the potential application value of walnut and for exploiting its metabolism pathway.

scholarly journals Carotenoids profile and total polyphenols in fruits of Pereskia aculeata Miller

2012 ◽  
Vol 34 (1) ◽  
pp. 234-238 ◽  
Author(s):  
Tânia da Silveira Agostini-Costa ◽  
Danielle Cristina Wondraceck ◽  
Wesley da Silveira Rocha ◽  
Dijalma Barbosa da Silva
Keyword(s):  
High Performance ◽  
Chemical Characterization ◽  
Array Detector ◽  
Total Polyphenols ◽  
Bioactive Substances ◽  
Photodiode Array Detector ◽  
Tropical America ◽  
Photodiode Array ◽  
First Time

Pereskia aculeata Mill. (Ora-pro-nóbis) is a native cactaceae from tropical America, whose leaves have high protein content. In Brazil it is found in all territorial extension between the states of Bahia and Rio Grande do Sul. Most studies have focused on chemical characterization of the leaves of this specie. The objective was to assess the carotenoids profile and the total polyphenols present in the fruits of P. aculeate. Carotenoids were determined by HPLC-PAD (high performance liquid chromatography - photodiode array detector), total polyphenols were determined by Folin-Ciocalteu and vanillin methods. Trans-β-carotene was the main carotenoid, followed by α-carotene, lutein and other minor carotenoids. It was found 64.9 ± 1.1 mg.100g-1 of gallic acid equivalent, 14.8 ± 0.2 mg.100g-1 of catechin equivalent. Carotenoid identification of P. aculeate fruits are presented here by the first time and indicate that these fruits can be researched as source of bioactive substances, especially antioxidant and provitamin A carotenoids.

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

Planta Medica ◽  
2020 ◽  
Author(s):  
Omer I. Fantoukh ◽  
Yan-Hong Wang ◽  
Abidah Parveen ◽  
Mohammed F. Hawwal ◽  
Gadah A. Al-Hamoud ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Dietary Supplements ◽  
High Performance ◽  
Moringa Oleifera ◽  
Negative Ion ◽  
Array Detector ◽  
Ionization Mass ◽  
Fractionation Process ◽  
Chromatography Method ◽  
Drumstick Tree

Abstract Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1 – 9 were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5) and quercetin 3-O-(6-O-malonyl)-β−D-glucopyranoside (6) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6. Niazirin (2) was detected in amounts between 0.010 – 0.049 mg/100 mg while compound 1 was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

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