scholarly journals Molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda) from Kamchatka sables

2017 ◽  
Vol 54 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Nina A. Tranbenkova ◽  
Sergei E. Spiridonov
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Intestinal Parasites ◽  
Mitochondrial Gene ◽  
Kamchatka Peninsula ◽  
Morphological Data ◽  
Lsu Rdna ◽  
Sem Images ◽  
Rdna Sequences

SummaryThe nematodes of the genus Baylisascaris are common intestinal parasites of sables (Martes (M.) zibellina kamtschadalica Birula, 1916) on the entire territory of Kamchatka peninsula. Partial sequences of Cox I mitochondrial gene were used for molecular characterization of these nematodes, which confirmed the identification based on morphological data as B. devosi Sprent, 1952. Phylogenetic relationships of this Baylisascaris species were also inferred from the ITS rDNA and LSU rDNA sequences. SEM images were provided for taxonomically important morphological features.

scholarly journals Morphological and molecular characterization of Orbilia pseudopolybrocha and O. tonghaiensis, two new species of Orbiliaceae from China

2020 ◽  
Vol 70 (4) ◽  
pp. 2664-2676 ◽  
Author(s):  
Ying Zhang ◽  
Min Qiao ◽  
H.O. Baral ◽  
Jianping Xu ◽  
Ke-Qin Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Morphological Traits ◽  
Its Sequences ◽  
Rdna Sequences ◽  
Two New Species ◽  
Asexual Morphs ◽  
Morphological And Molecular Characterization

This study examined over 20 fungal specimens of the Orbiliaceae (Orbiliomycetes) from different regions in China. Our analyses based on morphological traits and the ITS rDNA sequences revealed two new Orbilia species with drechslerella-like asexual morphs. These new species are able to trap nematodes with constricting rings. In addition, Orbilia cf. orientalis is reported as a new cryptic Chinese variant of European collections of O. orientalis. All three species are described and illustrated in detail in this paper. Their phylogenetic relationships with other orbiliaceous species were identified based on their ITS sequences.

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

Molecular characterization of Setaria digitata from Mithun (Bos frontalis)

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Jayanta Kumar Chamuah ◽  
Aseno Sakhrie ◽  
Sanjeevini Lama ◽  
Subhas Chandra ◽  
Gajanan M. Chigure ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Southeast Asia ◽  
Molecular Characterization ◽  
Peritoneal Cavity ◽  
Mitochondrial Gene ◽  
Tribal Population ◽  
Arunachal Pradesh ◽  
Setaria Digitata ◽  
Cultural Life

AbstractMithun is a strongly built hill animal of Southeast Asia and plays an important role in the socio-economic and cultural life of the tribal population. Setaria digitata isolated from peritoneal cavity of mithun both from Arunachal Pradesh and Nagaland were characterized based on conserved region of 12SrDNA, 28SrDNA and ITS-2 and mitochondrial gene CoxI. Based on sequence analysis, it was found to be 99% similarity with Srilankan isolate of S. digitata.

Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka

2019 ◽  
Author(s):  
Gurupada Balol ◽  
C Channakeshava ◽  
M S Patil
Keyword(s):  
16S Rdna ◽  
Molecular Characterization ◽  
Cicer Arietinum ◽  
Nested Pcr ◽  
Rdna Sequences ◽  
Pcr Product ◽  
16S Rdna Sequences ◽  
Axillary Proliferation ◽  
Pale Green

Chickpea plants showing phytoplasma symptoms were observed in the research plots at University of Agricultural Sciences, Dharwad, Karnataka, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Dharwad chickpea phytoplasma shared the highest nucleotide identity of (>98%) with Periwinkle phyllody16SrII-E (EU096500). This study indicated the association of ‘Candidatus Phytoplasma aurantifolia’ the 16SrII-E group infecting chickpea from Northern Karnataka.

Phylogeny of dracunculoid nematodes (Chromadorea: Rhabditida: Spirurina: Dracunculoidea) from some Eurasian freshwater fishes

Zootaxa ◽  
2020 ◽  
Vol 4858 (4) ◽  
pp. 521-541
Author(s):  
SERGEY G. SOKOLOV ◽  
ALEXANDER P. KALMYKOV ◽  
SVETLANA V. MALYSHEVA
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Relationship ◽  
Ribosomal Dna ◽  
Phylogenetic Relationships ◽  
Type Species ◽  
Freshwater Fishes ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Rdna Sequences ◽  
The Family

Sets of small ribosomal DNA (SSU rDNA) and large ribosomal DNA (LSU rDNA) sequences were obtained for Philometroides moraveci Vismanis & Yunchis, 1994, Philometra kotlani (Molnár, 1969), Philometra rischta Skrjabin, 1923, Philometra cf. obturans (Prenant, 1886) (Philometridae), Sinoichthyonema amuri (Garkavi, 1972), Agrachanus scardinii (Molnár, 1966), Kalmanmolnaria intestinalis (Dogiel & Bychowsky, 1934) and Skrjabillanus tincae Shigin & Shigina, 1958 (Skrjabillanidae). Phylogenetic analysis of SSU rDNA data shows that dracunculoid nematodes are divided into two well-supported clades designated as Clade I and Clade II, respectively. Clade I includes the type species of the genus Philonema Kuitunen-Ekbaum, 1933, some species from the family Daniconematidae Moravec & Køie, 1987 and two subfamilies of skrjabillanids, Skrjabillaninae Shigin & Shigina, 1958 and Esocineminae Moravec, 2006. Clade II unites species from the families Dracunculidae Stiles, 1907, Micropleuridae Baylis & Daubney, 1926 and Philometridae Baylis & Daubney, 1926. Within the Philometridae, there are several well-supported groups of species, one of which unites freshwater Philometra spp. from the Palearctic cyprinids, identified as P. kotlani, P rischta, P. ovata (Zeder, 1803) and P. cyprinirutili (Creplin, 1825). However, the phylogenetic relationships of most philometrids are unresolved. An analysis of partial SSU and LSU rDNA sequences indicates that there is no direct phylogenetic relationship between Agrachanus Tikhomirova, 1971 (type species Skrjabillanus scardinii Molnár, 1966) and Skrjabillanus Shigin & Shigina, 1958 (type species Sk. tincae), which means that the genus Agrachanus can be resurrected. Our study confirms that Philonematinae Ivashkin, Sobolev & Khromova, 1971 should be elevated to the family rank. We formally establish the family Philonematidae Ivashkin, Sobolev & Khromova, 1971 stat. nov. We also suggest combining the superfamilies Dracunculoidea Stiles, 1907 and Camallanoidea Railliet & Henry, 1915 into the infraorder Camallanomorpha Roberts, Janovy & Nadler, 2013. 

Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Paraná River, Brazil

2015 ◽  
Vol 90 (6) ◽  
pp. 693-697 ◽  
Author(s):  
L.S. Gasques ◽  
R.J. Graça ◽  
S.M.A.P. Prioli ◽  
R.M. Takemoto ◽  
A.J. Prioli
Keyword(s):  
Cryptic Species ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Genetic Data ◽  
Flood Plain ◽  
Fish Parasites ◽  
Coi Gene ◽  
Coi Sequences

AbstractUrocleidoides ectoparasites are mainly found on fish of the neotropical regions. Although molecular research on monogeneans is available, no genetic data exist characterizing species in the Urocleidoides genus. Some DNA sequences have been efficacious in systematic studies and in the reconstruction of phylogenies of fish parasites. Relevant roles have been given to the sequence of the mitochondrial gene of cytochrome c oxidase I (COI). This study characterized COI sequences of the parasites Urocleidoides malabaricusi and U. cuiabai in trahira fish Hoplias aff. malabaricus of the flood plain of the Upper River Paraná, Brazil. The two species under analysis were distinguished by sequencing and analysing a 420-bp fragment of the COI gene, which suggested the existence of the cryptic species U. malabaricusi.

scholarly journals Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences

2016 ◽  
Vol 8 (2) ◽  
pp. 185
Author(s):  
Nia Kurniawaty ◽  
Purnama Hidayat ◽  
Aunu Rauf
Keyword(s):  
Pest Management ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Biology Education ◽  
Morphological Data ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Vegetable Crops ◽  
Total Extraction

<p>Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of  Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps  DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis.  PCR programme was succesfully to amplified mt<em>COI</em> gene fragment at 710 bp. The length of mt<em>COI </em>gene of <em>Gynaikothrips uzeli, Haplothrips ganglbaueri</em>, and <em>Pseudophilothrips ichini</em> were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mt<em>COI </em>gene mitochondrial had successfully supported the morphological data. </p><p><strong>How to Cite</strong></p><p>Kurniawaty, N., Hidayat, P. &amp; Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 185-192.</p>

Phylogenetic relationships in the harmful dinoflagellate Cochlodinium polykrikoides (Gymnodiniales, Dinophyceae) inferred from LSU rDNA sequences

Harmful Algae ◽  
2008 ◽  
Vol 7 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Mitsunori Iwataki ◽  
Hisae Kawami ◽  
Koichiro Mizushima ◽  
Christina M. Mikulski ◽  
Gregory J. Doucette ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Lsu Rdna ◽  
Cochlodinium Polykrikoides ◽  
Rdna Sequences ◽  
Harmful Dinoflagellate
Sign in / Sign up

Export Citation Format

Share Document