Development of control material for hemoglobin analysis

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Sakorn Pornprasert ◽  
Kunyakan Kongthai ◽  
Jarurin Waneesorn ◽  
Kanokwan Jaiping ◽  
Kallayanee Treesuwan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Freeze Drying ◽  
Sucrose Solution ◽  
Control Material ◽  
Normal Individuals ◽  
Thalassemia Trait

AbstractThere is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)AWashed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at –20°C for 1, 15 and 30 days and for 3 months.The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbAThe lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis.

Development of hemoglobin typing control materials for laboratory investigation of thalassemia and hemoglobinopathies

2016 ◽  
Vol 54 (1) ◽  
Author(s):  
Sakorn Pornprasert ◽  
Monthathip Tookjai ◽  
Manoo Punyamung ◽  
Panida Pongpunyayuen ◽  
Kanokwan Jaiping
Keyword(s):  
Liquid Chromatography ◽  
Laboratory Investigation ◽  
High Performance ◽  
Hplc Analysis ◽  
Freeze Drying ◽  
Saline Solution ◽  
Sucrose Solution ◽  
The Stability ◽  
And Storage ◽  
Hb Bart's

AbstractTo date, the hemoglobin (Hb) typing control materials for laboratory investigation of thalassemia with low (1.8%–3.2%) and high (4%–6%) levels of HbAErythrocytes of blood samples containing Hb Bart’s, HbH, HbE, HbF, Hb Constant Spring (CS), Hb Hope, and Hb Q-Thailand were washed and dialysed with 0.85% saline solution. The erythrocytes were then lysed in 5% sucrose solution. The lyophilized Hb typing control materials were prepared by using a freeze drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized Hb was performed after the storage at −20 °C for 1 year and also after reconstitution and storage at 4 or −20 °C for 30 days. In addition, the Hb analysis was compared between the three different methods of HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE).Following a year of storage at −20 °C, the HPLC chromatograms of lyophilized Hb typing control materials showed similar patterns to the equivalent fresh whole blood. The stability of reconstituted Hb typing control materials was also observed through 30 days after reconstitution and storage at −20 °C. Moreover, the Hb typing control materials could be analyzed by three methods, HPLC, LPLC and CE. Even a degraded peak of HbCS was found on CE electropherogram.The lyophilized Hb typing control materials could be developed and used as control materials for investigation of thalassemia and hemoglobinopathies.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Effects of Baicalein on Prostanoid Generation from the Lung and Contractile Responses of the Trachea in Guinea Pig

1997 ◽  
Vol 25 (01) ◽  
pp. 37-50 ◽  
Author(s):  
Koji Miyamoto ◽  
Takeshi Katsuragi ◽  
Parhat Abdu ◽  
Tatsuo Furukawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Smooth Muscle ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
High Performance ◽  
Hplc Analysis ◽  
Antigen Challenge ◽  
Cyclooxygenase Inhibitor ◽  
Prostaglandin F ◽  
Stimulation Of

Effects of baicalein on release of slow reacting substance of anaphylaxis (SRS-A) or leukotriene (L T) from the sensitized guinea pig lung after antigen challenge and tonus of guinea pig tracheal muscles were studied. Baicalein inhibited release of SRS-A from sensitized guinea pig lung after antigen challenge. High-performance liquid chromatography (HPLC) analysis revealed that released SRS-A consisted of LTC 4 and D 4. Baicalein also reduced release of LTC 4 and D 4 from the sensitized lung after antigen challenge. Baicalein relaxed the isolated guinea pig tracheal smooth muscle contracted by LTD 4, carbachol or histamine. However, this compound produced a contraction when the tracheal muscle was contracted by prostaglandin F 2α ( PGF 2α). This contraction by baicalein was abolished by pretreatment with indomethacin, a cyclooxygenase inhibitor. Baicalein elicited a relaxation in the normal non-sensitized tracheal preparation but a contraction in the tissue isolated from actively sensitized guinea pig in 4 among 7 cases. Baicalein also produced a contraction in the trachea pretreated with phorbol dibutyrate and contracted by carbachol, which was eliminated after treatment with indomethacin. The results suggest that baicalein exerts action via, at least, two different mechanisms, the inhibition of releasing SRS-A (LTs) and direct relaxing effects on the trachea. Besides, baicalein seems to produce contraction under certain conditions, which may involve stimulation of the cyclooxygenase pathway.

scholarly journals Comparative Study of Dark Beer Before and After Freeze Drying Rehydration Cycle

2018 ◽  
Vol 72 (2) ◽  
pp. 123-127
Author(s):  
Ingmārs Cinkmanis ◽  
Guntis Gailums ◽  
Sanita Vucāne
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Organic Acids ◽  
High Performance ◽  
Freeze Drying ◽  
Gas Chromatography Mass Spectrometry ◽  
Before And After

Abstract The aim of the study was to compare dark beer before and after a freeze drying rehydration cycle. The pH, colour, and concentrations of extract, organic acids, and phenolic compounds were determined. Dark beer before and after freeze drying was analysed using standard brewing analysis methods of the Mitteleuropäische Brautechnische Analysenkommision (MEBAK) and two types of chromatography: high-performance liquid chromatography (HPLC) and gas chromatography mass spectrometry (GC/MS). Increase of pH was not observed in dark beer after freeze drying. Dissolved powdered beer in deionised water was clear, dark brown, without sediments, and reproduced the original commercially available dark beer, with original extract substance content 8.88%. Concentrations of the following organic acids were determined: oxalic, tartaric, quinic, malic, ascorbic, lactic, acetic, citric, fumaric, and succinic. Volatile compounds by GC/MS analysis were isolated from dark beer before and after freeze drying.

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