Gold nanoparticles in the clinical laboratory: principles of preparation and applications

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Hassan M.E. Azzazy ◽  
Mai M.H. Mansour ◽  
Tamer M. Samir ◽  
Ricardo Franco
Keyword(s):  
Gold Nanoparticles ◽  
Clinical Laboratory ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Practical Applications ◽  
Molecular Assays ◽  
Detection Of Biomarkers

AbstractIn order to meet the challenges of effective healthcare, the clinical laboratory is constantly striving to improve testing sensitivity while reducing the required time and cost. Gold nanoparticles (AuNPs) are proposed as one of the most promising tools to meet such goals. They have unique optophysical properties which enable sensitive detection of biomarkers, and are easily amenable to modification for use in different assay formats including immunoassays and molecular assays. Additionally, their preparation is relatively simple and their detection methods are quite versatile. AuNPs are showing substantial promise for effective practical applications and commercial utilization is already underway. This article covers the principles of preparation of AuNPs and their use for development of different diagnostic platforms.

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes

2011 ◽  
Vol 1301 ◽  
Author(s):  
Seong Yoon Lim ◽  
Jae Hong Kim ◽  
Joon Seok Lee ◽  
Chan Beum Park
Keyword(s):  
Gold Nanoparticles ◽  
Enzymatic Reaction ◽  
Optical Biosensor ◽  
Reducing Agents ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Fluorescent Substrate ◽  
Reaction Solution ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

ABSTRACTWe present a versatile and facile route for highly sensitive detection of analytes through coupling the enlargement of gold nanoparticles (Au NPs) with fluorescence decrease. The fluorescence intensity of dye molecules (e.g., fluorescein or rhodamine B) significantly decreased with the increasing concentration of reducing agents, such as hydrogen peroxide and hydroquinone. The sensitivity for the detection of reducing agents was much higher than other detection methods based on the absorbance measurement of enlarged gold nanoparticles or quantum dot-enzyme hybridization. We could successfully detect acetylthiocholine with the detection limit of several nM orders, using an enzymatic reaction by acetylcholinesterase, a key route for the detection of toxic organophosphate compounds. The fluorescence decreasing approach described in this work requires only a simple addition of fluorescence dye to the reaction solution without any chemical modification. The strategy of fluorescence decrease coupled with nanoparticle growth will be applied on the fluorescent substrate to develop detection templates for highly sensitive optical biosensor.

scholarly journals Correction: Poly(adenine)-mediated DNA-functionalized gold nanoparticles for sensitive detection of mercury ions in aqueous media

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22691-22691
Author(s):  
Jinjin Yin ◽  
Jiuchao Wang ◽  
Xiyue Yang ◽  
Tao Wu ◽  
Huashan Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Media ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Functionalized Gold Nanoparticles

Correction for ‘Poly(adenine)-mediated DNA-functionalized gold nanoparticles for sensitive detection of mercury ions in aqueous media’ by Jinjin Yin et al., RSC Adv., 2019, 9, 18728–18733, DOI: 10.1039/C9RA03041G.

scholarly journals A Survey on Deep Learning Based Methods and Datasets for Monocular 3D Object Detection

Electronics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 517
Author(s):  
Seong-heum Kim ◽  
Youngbae Hwang
Keyword(s):  
Deep Learning ◽  
Object Detection ◽  
Low Cost ◽  
Detection Methods ◽  
Future Research ◽  
3D Object ◽  
Practical Applications ◽  
Depth Sensors ◽  
Significant Research ◽  
3D Object Detection

Owing to recent advancements in deep learning methods and relevant databases, it is becoming increasingly easier to recognize 3D objects using only RGB images from single viewpoints. This study investigates the major breakthroughs and current progress in deep learning-based monocular 3D object detection. For relatively low-cost data acquisition systems without depth sensors or cameras at multiple viewpoints, we first consider existing databases with 2D RGB photos and their relevant attributes. Based on this simple sensor modality for practical applications, deep learning-based monocular 3D object detection methods that overcome significant research challenges are categorized and summarized. We present the key concepts and detailed descriptions of representative single-stage and multiple-stage detection solutions. In addition, we discuss the effectiveness of the detection models on their baseline benchmarks. Finally, we explore several directions for future research on monocular 3D object detection.

Naked-eye colorimetric detection of HCV RNA mediated by a 5′ UTR-targeted antisense oligonucleotide and plasmonic gold nanoparticles

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta
Keyword(s):  
Gold Nanoparticles ◽  
Antisense Oligonucleotide ◽  
Early Stage ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Naked Eye

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.

Gold Nanoparticles-Mediated Ratiometric Fluorescence Aptasensor for Ultra-sensitive Detection of Abscisic Acid

2021 ◽  
pp. 113311
Author(s):  
Yongqiang Shi ◽  
Lei Lin ◽  
Yuzhen Wei ◽  
Wenting Li ◽  
Pengcheng Nie ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Abscisic Acid ◽  
Sensitive Detection ◽  
Ratiometric Fluorescence

Highly sensitive and rapid detection of citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) using Cas12a-based methods

2021 ◽  
Author(s):  
Matthew Wheatley ◽  
Yong-Ping Duan ◽  
Yinong Yang
Keyword(s):  
Early Detection ◽  
Nucleic Acids ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Candidatus Liberibacter Asiaticus ◽  
Fluorescent Reporter ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Citrus Huanglongbing (HLB) or greening is one of the most devastating diseases of citrus worldwide. Sensitive detection of its causal agent, Candidatus Liberibacter asiaticus (CLas), is critical for early diagnosis and successful management of HLB. However, current nucleic acid-based detection methods are often insufficient for the early detection of CLas from asymptomatic tissue, and unsuitable for high-throughput and field-deployable diagnosis of HLB. Here we report the development of the Cas12a-based DETECTR (DNA endonuclease-targeted CRISPR trans reporter) assay for highly specific and sensitive detection of CLas nucleic acids from infected samples. The DETECTR assay, which targets the five-copy nrdB gene specific to CLas, couples isothermal amplification with Cas12a trans-cleavage of fluorescent reporter oligos and enables detection of CLas nucleic acids at the attomolar level. The DETECTR assay was capable of specifically detecting the presence of CLas across different infected citrus, periwinkle and psyllid samples, and shown to be compatible with lateral flow assay technology for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position the assay as a potentially suitable tool for early detection of CLas in infected regions.

scholarly journals Sensitive detection of aflatoxin B1 molecules on gold SPR chip surface using functionalized gold nanoparticles

2015 ◽  
Vol 43 (3) ◽  
pp. 426-437 ◽  
Author(s):  
A. Majzik ◽  
V. Hornok ◽  
D. Sebők ◽  
T. Bartók ◽  
L. Szente ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aflatoxin B1 ◽  
Sensitive Detection ◽  
Chip Surface ◽  
Functionalized Gold Nanoparticles
Sign in / Sign up

Export Citation Format

Share Document