BRAF analysis by fine needle aspiration biopsy of thyroid nodules improves preoperative identification of papillary thyroid carcinoma and represents a prognostic factor. A mono-institutional experience

2011 ◽  
Vol 49 (2) ◽  
Author(s):  
Maria Rosa Pelizzo ◽  
Isabella Merante Boschin ◽  
Susi Barollo ◽  
Gianmaria Pennelli ◽  
Antonio Toniato ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Prognostic Factor ◽  
Thyroid Carcinoma ◽  
Fine Needle Aspiration ◽  
Thyroid Nodules ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Institutional Experience

scholarly journals Prospective efficacy of molecular preoperative diagnostics of papillary thyroid carcinoma

2010 ◽  
Vol 17 (1-2) ◽  
pp. 35-39
Author(s):  
Svetlana V. MANKOVSKAYA ◽  
Yuri E. DEMIDCHIK ◽  
Shunichi YAMASHITA
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Fine Needle Aspiration ◽  
Preoperative Diagnosis ◽  
Thyroid Nodules ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Molecular Test ◽  
Papillary Thyroid ◽  
Fine Needle

Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules. Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing. Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged. Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place. Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test

FN1, GALE, MET, and QPCT overexpression in papillary thyroid carcinoma: Molecular analysis using frozen tissue and routine fine-needle aspiration biopsy samples

2010 ◽  
Vol 39 (8) ◽  
pp. 556-561 ◽  
Author(s):  
Cristina Aparecida Troques da Silveira Mitteldorf ◽  
Juliana Moreira de Sousa-Canavez ◽  
Kátia Ramos Moreira Leite ◽  
Celso Massumoto ◽  
Luis Heraldo Camara-Lopes
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Molecular Analysis ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Frozen Tissue
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