A simple, fast and inexpensive automated technique for measurement of plasma nitrite

2010 ◽  
Vol 48 (12) ◽  
Author(s):  
Renata da Silva Pereira ◽  
Sílvia Juliane Piva ◽  
Etiane Tatsch ◽  
Helena Kober ◽  
Patrícia Gomes ◽  
...  
Keyword(s):  
Automated Technique ◽  
Plasma Nitrite

scholarly journals Automated Assessment of Thoracic-Abdominal Asynchrony in Patients with Morquio Syndrome

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 880
Author(s):  
Madhavi V. Ratnagiri ◽  
Yan Zhu ◽  
Tariq Rahman ◽  
Mary Theroux ◽  
Shunji Tomatsu ◽  
...  
Keyword(s):  
Learning Algorithm ◽  
Assessment Methodology ◽  
Emergency Rooms ◽  
Multiple Organs ◽  
Difficulty Breathing ◽  
Causes Of Mortality ◽  
Morquio Syndrome ◽  
Automated Technique ◽  
Thoracic And Abdominal ◽  
Function Testing

Morquio syndrome is a rare disease caused by a disorder in the storage of mucopolysaccharides that affects multiple organs, including musculoskeletal, respiratory, cardiovascular, and digestive systems. Respiratory failure is one of the leading causes of mortality in Morquio patients; thus, respiratory function testing is vital to the management of the disease. An automated respiratory assessment methodology using the pneuRIP device and a machine-learning algorithm was developed. pneuRIP is a noninvasive approach that uses differences between thoracic and abdominal movements (thoracic-abdominal asynchrony) during respiration to assess respiratory status. The technique was evaluated on 17 patients with Morquio (9 females and 8 males) between the ages of 2 and 57 years. The results of the automated technique agreed with the clinical assessment in 16 out of the 17 patients. It was found that the inverse cumulative percentage representation of the time delay between the thorax and abdomen was the most critical variable for accurate evaluation. It was demonstrated that the technique could be successfully used on patients with Morquio who have difficulty breathing with 100% compliance. This technique is highly accurate, portable, noninvasive, and easy to administer, making it suitable for a variety of settings, such as outpatient clinics, at home, and emergency rooms.

Characterization of Textured Aluminum Lines and Modelling of Stress Voiding

1994 ◽  
Vol 343 ◽  
Author(s):  
S.C. Wardle ◽  
B.L. Adams ◽  
C.S. Nichols ◽  
D.A. Smith
Keyword(s):  
Free Energy ◽  
Excess Free Energy ◽  
Mean Field ◽  
High Energy ◽  
Polycrystalline Structure ◽  
Field Approach ◽  
Bamboo Structure ◽  
Automated Technique ◽  
Theory And Modeling

ABSTRACTIt is well known from studies of individual interfaces that grain boundaries exhibit a spectrum of properties because their structure is misorientation dependent. Usually this variability is neglected and properties are modeled using a mean field approach. The limitations inherent in this approach can be overcome, in principle, using a combination of experimental techniques, theory and modeling. The bamboo structure of an interconnect is a particularly simple polycrystalline structure that can now be readily characterized experimentally and modeled in the computer. The grain misorientations in a [111] textured aluminum line have been measured using the new automated technique of orientational imaging microscopy. By relating boundary angle to diffusivity the expected stress voiding failure processes can be predicted through the link between misorientation angle, grain boundary excess free energy and diffusivity. Consequently it can be shown that the high energy boundaries are the favored failure sites thermodynamically and kinetically.

Effects of Early Neuronal and Delayed Inducible Nitric Oxide Synthase Blockade on Cardiovascular, Renal, and Hepatic Function in Ovine Sepsis

2010 ◽  
Vol 113 (6) ◽  
pp. 1376-1384 ◽  
Author(s):  
Matthias Lange ◽  
Atsumori Hamahata ◽  
Daniel L. Traber ◽  
Yoshimitsu Nakano ◽  
Aimalohi Esechie ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Nitric Oxide Synthase Inhibitor ◽  
Nitrite Nitrate ◽  
Synthase Inhibitor ◽  
Plasma Nitrite ◽  
Oxide Synthase ◽  
Organ Dysfunctions ◽  
Inducible Nitric Oxide

Background Recent evidence suggests that nitric oxide produced via the neuronal nitric oxide synthase is involved mainly in the early response to sepsis, whereas nitric oxide derived from the inducible nitric oxide synthase is responsible during the later phase. We hypothesized that early neuronal and delayed inducible nitric oxide synthase blockade attenuates multiple organ dysfunctions during sepsis. Methods Sheep were randomly allocated to sham-injured, nontreated animals (n = 6); injured (48 breaths of cotton smoke and instillation of Pseudomonas aeruginosa into the lungs), nontreated animals (n = 7); and injured animals treated with a neuronal nitric oxide synthase inhibitor from 1 to 12 h and an inducible nitric oxide synthase inhibitor from 12 to 24 h postinjury (n = 6). Results The injury induced arterial hypotension, vascular leakage, myocardial depression, and signs of renal and hepatic dysfunctions. The treatment significantly attenuated, but did not fully prevent, the decreases in mean arterial pressure and left ventricular stroke work index. Although the elevation of creatinine levels was partially prevented, the decreases in urine output and creatinine clearance were not affected. The injury-related increases in bilirubin levels, international normalized ratio, and lipid peroxidation in liver tissue were significantly attenuated. Although plasma nitrite/nitrate levels were significantly increased versus baseline from 12-24 h in controls, plasma nitrite/nitrate levels were not increased in treated animals. Conclusions The combination treatment shows potential benefit on sepsis-related arterial hypotension and surrogate parameters of organ dysfunctions in sheep. It may be crucial to identify the time course of expression and activation of different nitric oxide synthase isoforms in future investigations.

The L-Arginine:Nitric Oxide Pathway Is the Major Source of Plasma Nitrite in Fasted Humans

1995 ◽  
Vol 211 (2) ◽  
pp. 705 ◽  
Author(s):  
P.M. Rhodes ◽  
A.M. Leone ◽  
P.L. Francis ◽  
A.D. Struthers ◽  
S. Moncada ◽  
...  
Keyword(s):  
Plasma Nitrite

scholarly journals Contrasting effects of intervention with ETA and ETB receptor antagonists in hypertension induced by angiotensin II and high-salt dietThis article is one of a selection of papers published in the two-part special issue entitled 20 Years of Endothelin Research.

2010 ◽  
Vol 88 (8) ◽  
pp. 802-807 ◽  
Author(s):  
Erika I. Boesen ◽  
Jennifer S. Pollock ◽  
David M. Pollock
Keyword(s):  
Angiotensin Ii ◽  
Creatinine Clearance ◽  
Nitrate Concentration ◽  
Therapeutic Approaches ◽  
Receptor Antagonists ◽  
Induced Hypertension ◽  
Etb Receptor ◽  
Nitrite Nitrate ◽  
Plasma Nitrite ◽  
Selection Of

Endothelin (ET) receptor antagonists are antihypertensive and renoprotective in angiotensin II (AngII)-induced hypertension if administered when AngII infusion commences, but their effects on established hypertension are poorly understood. We therefore tested the effects of intervening with an ETA (ABT-627) or ETB (A-192621) receptor antagonist after establishing hypertension with AngII (65 ng/min s.c.) plus 8% NaCl diet (AngII–HS) in rats. Prior to administration of ABT-627, AngII–HS and AngII–HS plus ABT-627 groups displayed robust hypertension (mean arterial pressure (MAP), 170 ± 5 and 165 ± 5 mm Hg versus 110 ± 3 mm Hg in normal salt control rats at day 7, P < 0.05). Administering ABT-627 from day 8 of AngII–HS treatment prevented further rises in MAP (168 ± 5 and 191 ± 3 mm Hg at day 13 in AngII–HS plus ABT-627 and AngII–HS, P < 0.001), without blunting the significant increases in urinary protein (19-fold), albumin (25-fold), or MCP-1 excretion (6- to 8-fold) or the reduction in creatinine clearance. Administering A-192621 from day 8 mildly exacerbated AngII–HS induced hypertension (P < 0.05 for AngII–HS versus AngII–HS plus A-192621 on days 11 and 12 only) and reduced plasma nitrite/nitrate concentration (P < 0.05), without affecting proteinuria, albuminuria, or creatinine clearance. These results confirm the importance of ETA receptor signaling in maintaining AngII–HS hypertension and suggest that including ETB receptor blockade in therapeutic approaches to treating hypertension would be ineffective or even counterproductive.

An Automated Technique for the Clearing and Staining of Fetal Bone in the Autotechnicon

1967 ◽  
Vol 126 (3) ◽  
pp. 819-821
Author(s):  
H. Rowe ◽  
E. Schwartz ◽  
G. C. Boxill
Keyword(s):  
Fetal Bone ◽  
Automated Technique

An Automated Technique for in Vitro Assessment of the Susceptibility of Urinary Catheter Materials to Encrustation

1987 ◽  
Vol 16 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A J Cox ◽  
D W L Hukins ◽  
K E Davies ◽  
J C Irlam ◽  
T M Sutton
Keyword(s):  
Chemical Composition ◽  
Urinary Catheter ◽  
Reaction Vessel ◽  
Indwelling Catheters ◽  
Artificial Urine ◽  
In Vitro Assessment ◽  
Infected Urine ◽  
Automated Technique

An automated technique has been developed for assessing the extent to which existing or potential materials for the construction of indwelling catheters become encrusted during exposure to infected urine. In this technique the enzyme urease is added to artificial urine containing albumin in a reaction vessel which contains the samples to be tested. Controlled replacement of reactants leads to appreciable formation of encrusting deposits which adhere firmly to the surface of the test samples. Deposits have the same chemical composition as those which encrust catheters in vivo.

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