Integrated PCR amplification and detection processes on a Lab-on-Chip platform: a new advanced solution for molecular diagnostics

2010 ◽  
Vol 48 (3) ◽  
Author(s):  
Barbara Foglieni ◽  
Angela Brisci ◽  
Floriana San Biagio ◽  
Patrizia Di Pietro ◽  
Salvatore Petralia ◽  
...  
Keyword(s):  
Molecular Diagnostics ◽  
Pcr Amplification ◽  
Lab On Chip ◽  
On Chip

Advanced microfluidic packaging for molecular diagnostics

2010 ◽  
Vol 2010 (1) ◽  
pp. 000036-000041
Author(s):  
M. Palmieri ◽  
T. Barbuzzi ◽  
A. Maierna ◽  
M. Marchi ◽  
G. Montalbano ◽  
...  
Keyword(s):  
Molecular Diagnostics ◽  
System Integration ◽  
Lab On Chip ◽  
Design Elements ◽  
Product Concept ◽  
Flexible System ◽  
Micro Channels ◽  
Commercial Applications ◽  
On Chip

STMicroelectronics has teamed up with Boehringer Ingelheim microParts GmbH to develop the In-Check™ Lab-on-Chip microfluidic disposable cartridge. In-Check™ is a ST proprietary platform dedicated to the in-vitro molecular diagnostics, e.g. biological analysis based on nucleic acid targets such as DNA, RNA. Its first generation has been released to commercial applications such as virus or bacteria borne infectious diseases. The second generation described herein will further enhance the platform customer experience by means of an innovative design of its disposable component. Indeed the new format represents a substantial step forward in the system integration and easy-of-use. This advanced microfluidics package enables fully automated application protocols such as analyte and reagents input, management and disposal. In-Check™ cartridge is a plastic-based self-contained embodiment which integrates a variety of design elements and components, including liquid loading and waste reservoirs, connecting micro-channels, two sets of valves one set to tight-seal biochemical reactors and the other set for fluid routing, hydrophobic membranes, fluorescent read-out window. Such microfluidic platform married with integrated Micro Electo Mechanical System (MEMS) device and software algoritms provides a highly flexible system to run complex biological assays as RNA Reverse Transcription (RT), DNA Polimerase Chain Reaction (PCR), Probe Hybridization and Detection. This paper will present the disposable product concept, key components and functionality together with the design and manufacturing challenge.

scholarly journals A Lab‐On‐chip Tool for Rapid, Quantitative, and Stage‐selective Diagnosis of Malaria

2021 ◽  
pp. 2004101
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  
Lab On Chip ◽  
On Chip

scholarly journals Integrated Magnetohydrodynamic Pump with Magnetic Composite Substrate and Laser-Induced Graphene Electrodes

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1113
Author(s):  
Mohammed Asadullah Khan ◽  
Jürgen Kosel
Keyword(s):  
Magnetic Flux ◽  
Flow Rate ◽  
Magnetic Composite ◽  
Propulsive Force ◽  
Closed Channel ◽  
Lab On Chip ◽  
Composite Substrate ◽  
On Chip ◽  
High Level ◽  
Moving Parts

An integrated polymer-based magnetohydrodynamic (MHD) pump that can actuate saline fluids in closed-channel devices is presented. MHD pumps are attractive for lab-on-chip applications, due to their ability to provide high propulsive force without any moving parts. Unlike other MHD devices, a high level of integration is demonstrated by incorporating both laser-induced graphene (LIG) electrodes as well as a NdFeB magnetic-flux source in the NdFeB-polydimethylsiloxane permanent magnetic composite substrate. The effects of transferring the LIG film from polyimide to the magnetic composite substrate were studied. Operation of the integrated magneto hydrodynamic pump without disruptive bubbles was achieved. In the studied case, the pump produces a flow rate of 28.1 µL/min. while consuming ~1 mW power.

scholarly journals Towards lab-on-chip ultrasensitive ethanol detection using photonic crystal waveguide operating in the mid infrared

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ali Rostamian ◽  
Ehsan Madadi-Kandjani ◽  
Hamed Dalir ◽  
Volker J. Sorger ◽  
Ray T. Chen
Keyword(s):  
Photonic Crystal ◽  
Slow Light ◽  
High Sensitivity ◽  
Guided Wave ◽  
Silicon On Insulator ◽  
Group Index ◽  
Photonic Crystal Waveguide ◽  
Mid Infrared ◽  
Lab On Chip ◽  
On Chip

Abstract Thanks to the unique molecular fingerprints in the mid-infrared spectral region, absorption spectroscopy in this regime has attracted widespread attention in recent years. Contrary to commercially available infrared spectrometers, which are limited by being bulky and cost-intensive, laboratory-on-chip infrared spectrometers can offer sensor advancements including raw sensing performance in addition to use such as enhanced portability. Several platforms have been proposed in the past for on-chip ethanol detection. However, selective sensing with high sensitivity at room temperature has remained a challenge. Here, we experimentally demonstrate an on-chip ethyl alcohol sensor based on a holey photonic crystal waveguide on silicon on insulator-based photonics sensing platform offering an enhanced photoabsorption thus improving sensitivity. This is achieved by designing and engineering an optical slow-light mode with a high group-index of n g  = 73 and a strong localization of modal power in analyte, enabled by the photonic crystal waveguide structure. This approach includes a codesign paradigm that uniquely features an increased effective path length traversed by the guided wave through the to-be-sensed gas analyte. This PIC-based lab-on-chip sensor is exemplary, spectrally designed to operate at the center wavelength of 3.4 μm to match the peak absorbance for ethanol. However, the slow-light enhancement concept is universal offering to cover a wide design-window and spectral ranges towards sensing a plurality of gas species. Using the holey photonic crystal waveguide, we demonstrate the capability of achieving parts per billion levels of gas detection precision. High sensitivity combined with tailorable spectral range along with a compact form-factor enables a new class of portable photonic sensor platforms when combined with integrated with quantum cascade laser and detectors.

Monitoring microbial growth on a microfluidic lab-on-chip with electrochemical impedance spectroscopic technique

2021 ◽  
Vol 23 (2) ◽  
Author(s):  
Subhan Shaik ◽  
Aarthi Saminathan ◽  
Deepak Sharma ◽  
Jagdish A Krishnaswamy ◽  
D Roy Mahapatra
Keyword(s):  
Microbial Growth ◽  
Electrochemical Impedance ◽  
Spectroscopic Technique ◽  
Lab On Chip ◽  
On Chip ◽  
Electrochemical Impedance Spectroscopic

scholarly journals Design Methodology of Passive In-Line Relays for Molecular Communication in Flow-Induced Microfluidic Channel

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 65
Author(s):  
Puneet Manocha ◽  
Gitanjali Chandwani
Keyword(s):  
Communication System ◽  
Communication Systems ◽  
Microfluidic Channel ◽  
Microfluidic Channels ◽  
Molecular Communication ◽  
External Energy ◽  
Lab On Chip ◽  
Molecular Communication Systems ◽  
Macro Scale ◽  
On Chip

Molecular communication is a bioinspired communication that enables macro-scale, micro-scale and nano-scale devices to communicate with each other. The molecular communication system is prone to severe signal attenuation, dispersion and delay, which leads to performance degradation as the distance between two communicating devices increases. To mitigate these challenges, relays are used to establish reliable communication in microfluidic channels. Relay assisted molecular communication systems can also enable interconnection among various entities of the lab-on-chip for sharing information. Various relaying schemes have been proposed for reliable molecular communication systems, most of which lack practical feasibility. Thus, it is essential to design and develop relays that can be practically incorporated into the microfluidic channel. This paper presents a novel design of passive in-line relay for molecular communication system that can be easily embedded in the microfluidic channel and operate without external energy. Results show that geometric modification in the microfluidic channel can act as a relay and restore the degraded signal up-to 28%.

scholarly journals Diagnosis of Malaria: A Lab‐On‐chip Tool for Rapid, Quantitative, and Stage‐selective Diagnosis of Malaria (Adv. Sci. 14/2021)

2021 ◽  
Vol 8 (14) ◽  
pp. 2170087
Author(s):  
Marco Giacometti ◽  
Francesca Milesi ◽  
Pietro Lorenzo Coppadoro ◽  
Alberto Rizzo ◽  
Federico Fagiani ◽  
...  
Keyword(s):  
Lab On Chip ◽  
On Chip

scholarly journals Development of a microfluidic design for an automatic lab-on-chip operation

2016 ◽  
Vol 20 (10) ◽  
Author(s):  
Nitipon Puttaraksa ◽  
Harry J. Whitlow ◽  
Mari Napari ◽  
Leena Meriläinen ◽  
Leona Gilbert
Keyword(s):  
Lab On Chip ◽  
On Chip
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