Fluorimetric determination of activity and isoenzyme composition of N-acetyl-β-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia

2006 ◽  
Vol 44 (7) ◽  
Author(s):  
Carmelo Tassi ◽  
Antonio Angelini ◽  
Tommaso Beccari ◽  
Enrico Capodicasa
Keyword(s):  
Seminal Plasma ◽  
Fluorimetric Determination ◽  
Isoenzyme Composition ◽  
Determination Of Activity ◽  
Infertile Patients ◽  
Secretory Azoospermia

AbstractClin Chem Lab Med 2006;44:843–7.

Determination of Interleukin-11 in Seminal Plasma and Elevated Il-11 in Seminal Plasma of Infertile Patients with Urogenital Infection

1998 ◽  
Vol 41 (3) ◽  
pp. 177-183 ◽  
Author(s):  
I. Matalliotakis ◽  
A. Goumenou ◽  
E. Koumantakis ◽  
D. Kyriakou ◽  
Y. Fragouli ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Urogenital Infection ◽  
Interleukin 11 ◽  
Infertile Patients

scholarly journals Assay of β-N-acetylhexosaminidase isoenzymes in different biological specimens by means of determination of their activation energies

1998 ◽  
Vol 44 (2) ◽  
pp. 226-231 ◽  
Author(s):  
Luis F Pérez ◽  
J Carlos Tutor
Keyword(s):  
Activation Energy ◽  
Cerebrospinal Fluid ◽  
Catalytic Activity ◽  
Seminal Plasma ◽  
Activation Energies ◽  
Thermodynamic Method ◽  
Heat Inactivation ◽  
Specific Substrate ◽  
Isoenzyme Composition

Abstract The activation energy (Ea) of β-N-acetylhexosaminidase (Hex, EC 3.2.1.52) was determined with 3,3′-dichlorophenylsulfonphthaleinyl-N-acetyl-β-d-glucosaminide as substrate, with a much higher value being found for the Hex B isoenzyme (Ea = 75.1 kJ/mol) than for the Hex A isoenzyme (Ea = 41.8 kJ/mol). This fact allowed for the development of a fast and reliable thermodynamic method to determine the isoenzyme composition of Hex in different biological specimens (serum/plasma, saliva, cerebrospinal fluid, seminal plasma, urine, and leukocyte lysates). The results in serum given by the proposed method may be superimposed upon those obtained by the heat inactivation assay of O’Brien et al. (N Engl J Med 1970;273:15–20), and the catalytic activity calculated for Hex A offers a good correlation with that obtained by using the specific substrate 4-methylumbelliferyl-N-acetyl-β-d-glucosaminide-6 sulfate (n = 25, r = 0.953).

The fluorimetric determination of cerium and terbium

1989 ◽  
Vol 54 (3) ◽  
pp. 616-621 ◽  
Author(s):  
Záviš Holzbecher
Keyword(s):  
Rare Earth ◽  
Detection Limit ◽  
Rare Earth Elements ◽  
Fluorimetric Determination ◽  
Fluorescence Maximum ◽  
Relative Standard ◽  
And Shifts ◽  
Hcl Medium ◽  
Fold Excess

It has been found that phosphoric acid decreases the first excitation maximum of Ce(III) at 256 nm, increases the second excitation maximum at 297 nm and shifts the fluorescence maximum from 350 to 346 nm. Under optimum conditions, with λexc = 297 nm and λem = 346 nm, Ce(III) can be determined fluorimetrically with a detection limit of 1.2 ng ml-1 in 12M-H3PO4 medium. No interference was observed from a 20-200 fold excess of HCl, H2SO4, Na, K, NH4+, Al and the rare earth elements. HNO3 interferes and Ce(IV) and Fe(III) interfere strongly. It follows from the stereofluorograms of Ce and Tb that the spectra of the two elements are practically independent. The detection limit for Tb(III) in 0.02-2.5M-H2SO4 medium for λexc = 222 nm and λem = 494 nm is 33 ng ml-1. No interference was observed from a 5-20 fold excess of Al3+ and the other rare earth elements. The determination is slightly less sensitive in H3PO4 or HCl medium. The relative standard deviation of the measurement for 10 ng ml-1 Ce(III) or 50 ng ml-1 Tb(III) is about 3%.

Fluorimetric Determination Of Mercury (Ii) Based On The Inhibition Of The Enzymatic Activity Of Urease

1994 ◽  
Vol 27 (5) ◽  
pp. 867-878 ◽  
Author(s):  
D. W. Bryce ◽  
J. M. Fernández-Romero ◽  
M. D. Luque de Castro
Keyword(s):  
Enzymatic Activity ◽  
Fluorimetric Determination
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