The influence of freezer storage of urine samples on the BONN-Risk-Index for calcium oxalate crystallization

2004 ◽  
Vol 42 (6) ◽  
Author(s):  
Norbert Laube ◽  
Diana J. Zimmermann
Keyword(s):  
Calcium Oxalate ◽  
Risk Index ◽  
Urinary Stone ◽  
Urinary Calcium ◽  
Urine Samples ◽  
Calcium Oxalate Crystallization ◽  
Crystallization Experiment ◽  
Bonn Risk Index ◽  
Crystallization Risk

AbstractThis study was performed to quantify the effect of a 1-weekfreezer storage of urine on its calcium oxalate crystallization risk. Calcium oxalate is the most common urinary stone material observed in urolithiasis patients in western and affluent countries. The BONN-Risk-Index of calcium oxalate crystallization risk in human urine is determined from a crystallization experiment performed on untreated native urine samples.We tested the influence of a 1-weekfreezing on the BONN-Risk-Index value as well as the effect of the sample freezing on the urinary osmolality. In vitro crystallization experiments in 49 native urine samples from stone-forming and non-stone forming individuals were performed in order to determine their calcium oxalate crystallization risk according to the BONN-Risk-Index approach. Comparison of the results derived from original sample investigations with those obtained from the thawed aliquots by statistical evaluation shows that i) no significant deviation from linearity between both results exists and ii) both results are identical by statistical means. This is valid for both, the BONN-Risk-Index and the osmolality data.The differences in the BONN-Risk-Index results of both procedures of BONN-Risk-Index determination, however, exceed the clinically acceptable difference. Thus, determination of the urinary calcium oxalate crystallization risk from thawed urine samples cannot be recommended.

Bonn Risk Index Based Micromethod for Assessing Risk of Urinary Calcium Oxalate Stone Formation

2010 ◽  
Vol 183 (3) ◽  
pp. 1157-1162 ◽  
Author(s):  
T. Porowski ◽  
P. Mrozek ◽  
J. Sidun ◽  
W. Zoch-Zwierz ◽  
J. Konstantynowicz ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Stone Formation ◽  
Risk Index ◽  
Urinary Calcium ◽  
Calcium Oxalate Stone ◽  
Bonn Risk Index

Tamm-Horsfall Mucoprotein Reduces Promotion of Calcium Oxalate Crystal Aggregation Induced by Urate in Human Urine In Vitro

1994 ◽  
Vol 87 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Phulwinder K. Grover ◽  
Villis R. Marshall ◽  
Rosemary L. Ryall
Keyword(s):  
Calcium Oxalate ◽  
Stone Formation ◽  
Urine Samples ◽  
Urine Specimen ◽  
Calcium Oxalate Crystal ◽  
Physiological Concentration ◽  
Calcium Oxalate Crystallization ◽  
Crystal Aggregation

1. Increasing the concentration of dissolved urate promotes calcium oxalate crystallization in urine from which Tamm-Horsfall mucoprotein, an inhibitor of calcium oxalate crystal aggregation, has almost completely been removed. This study aimed to determine whether the effect of urate could be reduced or abolished by a physiological concentration of Tamm-Horsfall mucoprotein. This was approached in two ways. 2. The effect of Tamm-Horsfall mucoprotein on calcium oxalate crystallization induced by urate was tested in ultrafiltered (10 kDa) urine samples from 10 healthy men. Tamm-Horsfall mucoprotein (35 mg/l) was added to half of each specimen, the urate concentration was increased by the addition of sodium urate solution and crystallization was induced by a standard load of oxalate. The remainder of each urine specimen was used as a control; these specimens were treated with an identical amount of urate solution, but contained no Tamm-Horsfall mucoprotein. Tamm-Horsfall mucoprotein had no effect on the urinary metastable limit or on the deposition of calcium oxalate, but significantly reduced the size of the particles precipitated. 3. The effect of increasing the urate concentration in the presence of Tamm-Horsfall mucoprotein was tested. Tamm-Horsfall mucoprotein (35 mg/l) was added to 10 ultrafiltered urine samples as before, the samples were divided, and the concentration of urate was increased in half of each specimen. Compared with the control to which no urate was added, urate significantly reduced the amount of oxalate required to induce spontaneous calcium oxalate nucleation and increased the median volume and the particle size of the material deposited. 4. It was concluded that, in vivo, (a) hyperuricosuria would encourage the formation of calcium oxalate stones by promoting calcium oxalate crystallization, (b) Tamm-Horsfall mucoprotein would not lessen the effect of urate on calcium oxalate nucleation or bulk deposition but would reduce its effect on crystal aggregation; it could therefore reduce the likelihood of stone formation in patients with hyperuricosuria.

Calcium oxalate crystallization in untreated urine, centrifuged and filtered urine and ultrafiltered urine

2004 ◽  
Vol 42 (1) ◽  
Author(s):  
Angela Guerra ◽  
Tiziana Meschi ◽  
Franca Allegri ◽  
Tania Schianchi ◽  
Giuditta Adorni ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Urine Samples ◽  
Normal Male ◽  
Calcium Oxalate Crystallization ◽  
Different Types

AbstractCentrifuged and filtered urine is often used to evaluate in vitro the crystallization processes of calcium oxalate (CaOx), but even such simple manipulations can alter the composition of the urine, as regards its protein and lipid concentrations. In urine samples taken from 17 normal male adults, we evaluated CaOx crystallization by simultaneously using three different types of urine: untreated (U), centrifuged at 2000 rpm (800

Determination of Urinary Calcium-Oxalate Formation Risk with BONN-Risk-Index and EQUIL Applied to a Family

2002 ◽  
Vol 42 (3) ◽  
pp. 633-639 ◽  
Author(s):  
Norbert Laube ◽  
Vera Labedzke ◽  
Stefan Hergarten ◽  
Albrecht Hesse
Keyword(s):  
Calcium Oxalate ◽  
Risk Index ◽  
Urinary Calcium ◽  
Bonn Risk Index

scholarly journals INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION BY AN EXTRACT OF OCIMUM BASILICUM SEEDS: AN IN-VITRO STUDY

2020 ◽  
pp. 166-169
Author(s):  
PATEL RAVINDRAKUMAR K ◽  
PATEL SANDIP B
Keyword(s):  
Calcium Oxalate ◽  
Human Urine ◽  
In Vitro Study ◽  
Urinary Stone ◽  
Ocimum Basilicum ◽  
Sodium Oxalate ◽  
Historical Time ◽  
Calcium Oxalate Crystallization ◽  
Normal Human

Objective: Ocimum basilicum (OB) has been used to treat diverse illnesses which include urinary stone disorder for a reason that historical time in India. We investigated OB seeds for antiurolithic activity. Methods: Calcium oxalate crystallization becomes triggered by the addition of 0.01 M sodium oxalate answers in normal human urine and nucleation was done. Results: OB seeds were discovered to be robust and promising antiurolithiatic agents which are in accordance with its use in traditional medication. Conclusion: An extract of the traditional herb OB has super inhibitory activity on crystalluria and therefore might be useful in dissolving urinary stone; however, in addition, a study in animal fashions of urolithiasis is needed to assess its capability antiurolithiatic interest.

scholarly journals Normative data on the Bonn Risk Index for calcium oxalate crystallization in healthy children

2007 ◽  
Vol 22 (4) ◽  
pp. 514-520 ◽  
Author(s):  
Tadeusz Porowski ◽  
Walentyna Zoch-Zwierz ◽  
Anna Wasilewska ◽  
Anthony Spotyk ◽  
Jerzy Konstantynowicz
Keyword(s):  
Calcium Oxalate ◽  
Normative Data ◽  
Risk Index ◽  
Healthy Children ◽  
Calcium Oxalate Crystallization ◽  
Bonn Risk Index

Urine osmolality predicts calcium-oxalate crystallization risk in patients with recurrent urolithiasis

Urolithiasis ◽  
2021 ◽  
Author(s):  
Stavros A. Kavouras ◽  
Hyun-Gyu Suh ◽  
Marion Vallet ◽  
Michel Daudon ◽  
Andy Mauromoustakos ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Urine Osmolality ◽  
Calcium Oxalate Crystallization ◽  
Crystallization Risk

Expression of proteins that inhibit calcium oxalate crystallization in vitro in the urine of normal and stone-forming individuals

2001 ◽  
Vol 37 (1) ◽  
pp. 104-112 ◽  
Author(s):  
Ryan C. Hedgepeth ◽  
Lizhu Yang ◽  
Martin I. Resnick ◽  
Susan Ruth Marengo
Keyword(s):  
Calcium Oxalate ◽  
Calcium Oxalate Crystallization
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