Comparative Nuclear Magnetic Resonance Studies of Water Permeability of Red Blood Cells from Maternal Venous Blood and Newborn Umbilical Cord Blood

2001 ◽  
Vol 39 (7) ◽  
Author(s):  
Gheorghe Benga ◽  
Lucian Frenţescu ◽  
Horea Matei ◽  
œtefan Ţigan
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Water Permeability ◽  
Blood Cells ◽  
Venous Blood ◽  
Magnetic Resonance Studies

scholarly journals Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

2019 ◽  
Vol 20 (19) ◽  
pp. 4875 ◽  
Author(s):  
Vanegas ◽  
Galindo ◽  
Páez-Gutiérrez ◽  
González-Acero ◽  
Medina-Valderrama ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cell Viability ◽  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Leukocyte ◽  
Hla Typing ◽  
Leukocyte Antigen

Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

Potential use of autologous umbilical cord blood red blood cells for early transfusion needs of premature infants

Transfusion ◽  
2006 ◽  
Vol 46 (6) ◽  
pp. 1049-1056 ◽  
Author(s):  
Marijke Jansen ◽  
Anneke Brand ◽  
Jeannette S. von Lindern ◽  
Sicco Scherjon ◽  
Frans J. Walther
Keyword(s):  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Premature Infants ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Potential Use

scholarly journals A Nuclear Magnetic Resonance and Fourier-Transform Infrared Spectroscopic Study of Lyophilized Umbilical Cord Blood Platelet Rich Plasma

2021 ◽  
Vol 11 (3) ◽  
pp. 3854-3860
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Fourier Transform ◽  
Magnetic Resonance ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Platelet Rich Plasma ◽  
Fourier Transform Infrared ◽  
Blood Platelet ◽  
Nuclear Magnetic

Cord blood platelet-rich plasma is regarded as a potential therapeutic agent with wound healing and regenerative purposes. It has many advantages, such as availability, universal use, and being rich in regenerative tissue factors. To characterize the Umbilical Cord Blood Platelet Rich Plasma (UCB-PRP), umbilical cord blood is collected from healthy mothers in sterile vacutainers. Platelet-rich plasma was extracted after two sets of centrifugations and lyophilized using the freeze-drying method. The samples are subjected to physicochemical characterization using Nuclear Magnetic Resonance (NMR) and Fourier-Transform Infrared (FTIR) Spectroscopy. This translational approach provided evidence of clinical effectiveness for understanding physiology and pathophysiology at the molecular level. Accordingly, FTIR and NMR studies provided information on the structural stability of protein molecules present in UCB-PRP.

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