Antioxidant Interferences in Superoxide Dismutase Activity Methods Using Superoxide Radical as Substrate

1998 ◽  
Vol 36 (6) ◽  
Author(s):  
İlker Durak ◽  
Orhan Canbolat ◽  
Murat Kaçmaz ◽  
Gülhan Özgen ◽  
Hasan Serdar Öztürk
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Radical ◽  
Superoxide Dismutase Activity

Reaotive oxygen generation by azomethine H: a new antimalarial drug

1995 ◽  
Vol 73 (8) ◽  
pp. 1189-1194 ◽  
Author(s):  
Ethel L. B. Novelli ◽  
Assunta M. M. Silva ◽  
Jose L.V.B. Novell F. ◽  
Paulo R. Curi
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Superoxide Radical ◽  
Alanine Transaminase ◽  
Superoxide Dismutase Activity ◽  
Acid Solutions ◽  
Oxygen Generation ◽  
Hydrochloric Acid Solutions ◽  
Superoxide Free Radical ◽  
Important Intermediate

Superoxide radical [Formula: see text] is a free radical that may be involved in various toxic processes. Cu—Zn superoxide dismutase catalyzes the dismutation of the superoxide free radical and protects cells from oxidative damage. A rat bioassay validated for the identification of the toxic effects of azomethine H revealed increased serum activities of amylase, alanine transaminase, and alkaline phosphatase. The lipoperoxide and bilirubin concentrations were also increased in animals that received azomethine H (1 g/kg) from ascorbic or hydrochloric acid solutions. Azomethine H increased Cu–Zn superoxide dismutase activity. This elevation of Cu–Zn superoxide dismutase activity was highest on the 7th day and was at levels comparable with those of control rats from day 60 onwards. Superoxide is an important intermediate in the action and toxicity of azomethine H.Key words: azomethine H, superoxide radical, antimalarial, toxicity.

Diazoxide Modulates Cardiac Hypertrophy by Targeting H2O2 Generation and Mitochondrial Superoxide Dismutase Activity

2020 ◽  
Vol 13 (1) ◽  
pp. 76-83
Author(s):  
Aline Maria Brito Lucas ◽  
Joana Varlla de Lacerda Alexandre ◽  
Maria Thalyne Silva Araújo ◽  
Cicera Edna Barbosa David ◽  
Yuana Ivia Ponte Viana ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Cardiac Hypertrophy ◽  
Superoxide Dismutase Activity ◽  
Heart Weight ◽  
Pharmacological Intervention ◽  
Wall Thickening ◽  
H2o2 Production ◽  
Mitochondrial Superoxide ◽  
Mitochondrial Superoxide Dismutase

Background: Cardiac hypertrophy involves marked wall thickening or chamber enlargement. If sustained, this condition will lead to dysfunctional mitochondria and oxidative stress. Mitochondria have ATP-sensitive K+ channels (mitoKATP) in the inner membrane that modulate the redox status of the cell. Objective: We investigated the in vivo effects of mitoKATP opening on oxidative stress in isoproterenol- induced cardiac hypertrophy. Methods: Cardiac hypertrophy was induced in Swiss mice treated intraperitoneally with isoproterenol (ISO - 30 mg/kg/day) for 8 days. From day 4, diazoxide (DZX - 5 mg/kg/day) was used in order to open mitoKATP (a clinically relevant therapy scheme) and 5-hydroxydecanoate (5HD - 5 mg/kg/day) or glibenclamide (GLI - 3 mg/kg/day) were used as mitoKATP blockers. Results: Isoproterenol-treated mice had elevated heart weight/tibia length ratios (HW/TL). Additionally, hypertrophic hearts had elevated levels of carbonylated proteins and Thiobarbituric Acid Reactive Substances (TBARS), markers of protein and lipid oxidation. In contrast, mitoKATP opening with DZX avoided ISO effects on gross hypertrophic markers (HW/TL), carbonylated proteins and TBARS, in a manner reversed by 5HD and GLI. Moreover, DZX improved mitochondrial superoxide dismutase activity. This effect was also blocked by 5HD and GLI. Additionally, ex vivo treatment of isoproterenol- induced hypertrophic cardiac tissue with DZX decreased H2O2 production in a manner sensitive to 5HD, indicating that this drug also acutely avoids oxidative stress. Conclusion: Our results suggest that diazoxide blocks oxidative stress and reverses cardiac hypertrophy. This pharmacological intervention could be a potential therapeutic strategy to prevent oxidative stress associated with cardiac hypertrophy.

scholarly journals Two New Compounds From the Heartwood of Dalbergia melanoxylon and Their Protective Effect on Hypoxia/Reoxygenation Injury in H9c2

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098777
Author(s):  
Yang Liu ◽  
Ni Zhang ◽  
Jun-wei He ◽  
Lan-ying Chen ◽  
Li Yang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lactate Dehydrogenase ◽  
Protective Effect ◽  
Superoxide Dismutase Activity ◽  
Spectral Evidence ◽  
Reoxygenation Injury ◽  
Dalbergia Melanoxylon ◽  
New Compounds ◽  
Hypoxia Reoxygenation

A new neoflavonoid, named as (7 R)-(-)-3′,5-dihydroxy-4′,2,4-trimethoxy-dalbergiquinol (1) and a new phenanthrenedione, named as 3′,7-dihydroxy-3,6- dimethoxy-9-phenyl-1,4-phenanthrenedione (2), together with 4 known compounds, 5- O-methyldalbergiphenol (3), 3′,7-dihydroxy-4′,3,6-trimethoxy-9-phenyl-1,4-phenanthrenedione (4), (+)-obtusafuran (5), and melanoxin (6) were isolated from the heartwood of Dalbergia melanoxylon. Their structures were elucidated on the basis of chemical and spectral evidence, as well as by comparison with literature data. Moreover, compound 1 showed a protective effect on hypoxia/reoxygenation injury in H9c2 at 10.0 μM by decreasing lactate dehydrogenase and malondialdehyde activity and enhancing superoxide dismutase activity.

scholarly journals Severe reduction of superoxide dismutase activity in the yeastSaccharomyces cerevisaewith the deletion or overexpression ofGTS1

2003 ◽  
Vol 223 (1) ◽  
pp. 141-145 ◽  
Author(s):  
Abudokirim Abudugupur ◽  
Zhaojun Xu ◽  
Kazuhiro Mitsui ◽  
Harumi Hisaki ◽  
Nobuo Ueda ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
Severe Reduction

scholarly journals A Germin-Like Protein Gene (CchGLP) of Capsicum chinense Jacq. Is Induced during Incompatible Interactions and Displays Mn-Superoxide Dismutase Activity

2011 ◽  
Vol 12 (11) ◽  
pp. 7301-7313 ◽  
Author(s):  
Fabiola León-Galván ◽  
Ahuizolt de Jesús Joaquín-Ramos ◽  
Irineo Torres-Pacheco ◽  
Ana P. Barba de la Rosa ◽  
Lorenzo Guevara-Olvera ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Protein Gene ◽  
Superoxide Dismutase Activity ◽  
Capsicum Chinense ◽  
Incompatible Interactions
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