Extracts of macroalgae from the Brazilian coast inhibit bacterial quorum sensing

2014 ◽  
Vol 57 (6) ◽  
Author(s):  
Daniela Batista ◽  
Ana Polycarpa Carvalho ◽  
Rafaela Costa ◽  
Ricardo Coutinho ◽  
Sergey Dobretsov
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Brazilian Coast ◽  
Acyl Homoserine Lactone ◽  
Associated Bacteria ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Polar Extracts

AbstractThe ability of polar (1:1 water/methanol) and non-polar (dichloromethane) extracts of macroalgae from Arraial do Cabo, RJ, Brazil, to inhibit quorum sensing (QS) was investigated. Before extraction, half of the algae were treated with 30% ethanol to kill and remove surface-associated bacteria. Twenty of 22 polar extracts inhibited the QS of the acyl homoserine lactone (AHL) producer and reporter

scholarly journals Structural Basis and Specificity of Acyl-Homoserine Lactone Signal Production in Bacterial Quorum Sensing

2002 ◽  
Vol 9 (3) ◽  
pp. 685-694 ◽  
Author(s):  
William T. Watson ◽  
Timothy D. Minogue ◽  
Dale L. Val ◽  
Susanne Beck von Bodman ◽  
Mair E.A. Churchill
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Structural Basis ◽  
Acyl Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Signal Production ◽  
Bacterial Quorum

scholarly journals Multiple N-Acyl Homoserine Lactone Signals of Rhizobium leguminosarum Are Synthesized in a Distinct Temporal Pattern

2001 ◽  
Vol 183 (23) ◽  
pp. 6771-6777 ◽  
Author(s):  
Renée S. Blosser-Middleton ◽  
Kendall M. Gray
Keyword(s):  
Quorum Sensing ◽  
Temporal Pattern ◽  
Rhizobium Leguminosarum ◽  
Homoserine Lactone ◽  
Global Perspective ◽  
Acyl Homoserine Lactone ◽  
Regulation Of Biosynthesis ◽  
Bacterial Quorum Sensing ◽  
Redundant Gene ◽  
Bacterial Quorum

ABSTRACT A common form of bacterial quorum sensing involves the production and release of acyl homoserine lactone (AHL) signal metabolites. The nitrogen-fixing symbiont Rhizobium leguminosarum reportedly produces at least six different AHLs, but little is known about the regulation of biosynthesis of these molecules. We used a radiolabeling protocol to quantify the relative amounts of AHLs synthesized over time by R. leguminosarum cells with and without the symbiosis plasmid pRL1JI. Cells containing pRL1JI were found to produce three predominant signals. In decreasing order of abundance, these wereN-(3-oxo)octanoyl homoserine lactone [(3-O)C8HSL], N-octanoyl homoserine lactone, and N-hexanoyl homoserine lactone. Cells without pRL1JI produced only two major signals,N-(3-hydroxy-7-cis)tetradecanoyl homoserine lactone [(3-OH)C14:1HSL] and (3-O)C8HSL. Each AHL exhibited a distinct temporal pattern of synthesis, suggesting that each AHL is subject to unique regulatory mechanisms. While (3-O)C8HSL was produced in both cultures, the patterns of synthesis were different in cells with and without pRL1JI, possibly as a result of redundant gene functions that are present on both the chromosome and the symbiosis plasmid. None of the AHLs appeared to regulate its own biosynthesis, although exogenous (3-OH)C14:1HSL did activate synthesis of the three AHLs made by cells containing pRL1JI. These results indicate that the synthesis of multiple AHLs in R. leguminosarum is regulated by complex mechanisms that operate independently of quorum sensing itself but that (3-OH)C14:1HSL can supersede these controls in pRL1JI-containing cells. This work provides an important global perspective for AHL regulation that both complements and contrasts with the results of previous studies performed with isolated gene systems.

scholarly journals N-Acyl Homoserine Lactone-Mediated Quorum Sensing with Special Reference to Use of Quorum Quenching Bacteria in Membrane Biofouling Control

2014 ◽  
Vol 2014 ◽  
pp. 1-25 ◽  
Author(s):  
Harshad Lade ◽  
Diby Paul ◽  
Ji Hyang Kweon
Keyword(s):  
Quorum Sensing ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Reactor Performance ◽  
Severe Problem ◽  
Membrane Biofouling ◽  
Bacterial Quorum Sensing ◽  
Quenching Mechanisms ◽  
Bacterial Quorum

Membrane biofouling remains a severe problem to be addressed in wastewater treatment systems affecting reactor performance and economy. The finding that many wastewater bacteria rely onN-acyl homoserine lactone-mediated quorum sensing to synchronize their activities essential for biofilm formations; the quenching bacterial quorum sensing suggests a promising approach for control of membrane biofouling. A variety of quorum quenching compounds of both synthetic and natural origin have been identified and found effective in inhibition of membrane biofouling with much less environmental impact than traditional antimicrobials. Work over the past few years has demonstrated that enzymatic quorum quenching mechanisms are widely conserved in several prokaryotic organisms and can be utilized as a potent tool for inhibition of membrane biofouling. Such naturally occurring bacterial quorum quenching mechanisms also play important roles in microbe-microbe interactions and have been used to develop sustainable nonantibiotic antifouling strategies. Advances in membrane fabrication and bacteria entrapment techniques have allowed the implication of such quorum quenching bacteria for better design of membrane bioreactor with improved antibiofouling efficacies. In view of this, the present paper is designed to review and discuss the recent developments in control of membrane biofouling with special emphasis on quorum quenching bacteria that are applied in membrane bioreactors.

scholarly journals Conformational studies of Gram-negative bacterial quorum sensing 3-oxo N -acyl homoserine lactone molecules

2017 ◽  
Vol 25 (16) ◽  
pp. 4285-4296 ◽  
Author(s):  
Darren Crowe ◽  
Alan Nicholson ◽  
Adrienne Fleming ◽  
Ed Carey ◽  
Goar Sánchez-Sanz ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Gram Negative ◽  
Conformational Studies ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

scholarly journals Antibody Interference withN-Acyl Homoserine Lactone-Mediated Bacterial Quorum Sensing

2006 ◽  
Vol 128 (9) ◽  
pp. 2802-2803 ◽  
Author(s):  
Gunnar F. Kaufmann ◽  
Rafaella Sartorio ◽  
Sang-Hyeup Lee ◽  
Jenny M. Mee ◽  
Laurence J. Altobell ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

scholarly journals Host modification of a bacterial quorum-sensing signal induces a phenotypic switch in bacterial symbionts

2017 ◽  
Vol 114 (40) ◽  
pp. E8488-E8497 ◽  
Author(s):  
Cleo Pietschke ◽  
Christian Treitz ◽  
Sylvain Forêt ◽  
Annika Schultze ◽  
Sven Künzel ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Communities ◽  
Homoserine Lactone ◽  
Innate Immune ◽  
Expression Data ◽  
Phenotypic Switch ◽  
Host Colonization ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Signals

Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a eukaryotic mechanism that enables Hydra to specifically modify long-chain 3-oxo-homoserine lactones into their 3-hydroxy-HSL counterparts. Expression data revealed that Hydra’s main bacterial colonizer, Curvibacter sp., responds differentially to N-(3-hydroxydodecanoyl)-l-homoserine lactone (3OHC12-HSL) and N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL). Investigating the impacts of the different N-acyl-HSLs on host colonization elucidated that 3OHC12-HSL allows and 3OC12-HSL represses host colonization of Curvibacter sp. These results show that an animal manipulates bacterial quorum-sensing signals and that this modification leads to a phenotypic switch in the bacterial colonizers. This mechanism may enable the host to manipulate the gene expression and thereby the behavior of its bacterial colonizers.

scholarly journals Genetic Differences in Barley Govern the Responsiveness to N-Acyl Homoserine Lactone

2019 ◽  
Vol 3 (3) ◽  
pp. 191-202 ◽  
Author(s):  
Abhishek Shrestha ◽  
Ahmed Elhady ◽  
Shimaa Adss ◽  
Gwendolin Wehner ◽  
Christoph Böttcher ◽  
...  
Keyword(s):  
Map Kinases ◽  
Plant Protection ◽  
Homoserine Lactone ◽  
Subsequent Treatment ◽  
Acyl Homoserine Lactone ◽  
Beneficial Effects ◽  
Enhanced Resistance ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Barley Genotypes

Enhanced resistance in barley (Hordeum vulgare) against pathogens, such as the powdery mildew-causing fungus Blumeria graminis f. sp. hordei, is of high importance. The beneficial effects of bacterial quorum sensing molecules on resistance and plant growth have been shown in different plant species. Here, we present the effects of the N-3-oxotetradecanoyl-l-homoserine lactone (oxo-C14-HSL) on the resistance of different barley genotypes. Genetically diverse accessions of barley were identified and exposed to the beneficial, oxo-C14-HSL-producing bacterium Ensifer meliloti or the pure N-acyl homoserine lactone (AHL) molecule. Metabolic profiling along with expression analysis of selected genes and physiological assays revealed that the capacity to react varies among different barley genotypes. We demonstrate that upon pretreatment with AHL molecule, AHL-primable barley genotype expresses enhanced resistance against B. graminis f. sp. hordei. We further show that pretreatment with AHL correlates with stronger activation of barley MAP kinases and regulation of defense-related PR1 and PR17b genes after a subsequent treatment with chitin. Noticeable was the stronger accumulation of lignin. Our results suggest that appropriate genetic background is required for AHL-induced priming. At the same time, they bear potential to use these genetic features for new breeding and plant protection approaches.

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