Section plane selection influences the results of histomorphometric studies: the example of dental implants

2012 ◽  
Vol 57 (5) ◽  
Author(s):  
Sigmar Kopp ◽  
Mareike Warkentin ◽  
Ferenc Öri ◽  
Peter Ottl ◽  
Günther Kundt ◽  
...  
Keyword(s):  
Section Plane ◽  
Section Thickness ◽  
Implant Surface ◽  
Bone Implant ◽  
Osseointegrated Implants ◽  
Section Technique ◽  
Significant Difference ◽  
Bone To Implant Contact ◽  
The Mean ◽  
Dependent Samples

AbstractThis study was designed to determine and statistically analyze bone-to-implant contact (BIC) values for human specimens segmented in at least two different locations.Samples of human bone with fractured osseointegrated implants were obtained from six patients. Sections were prepared, dehydrated, and resin infiltrated. Undecalcified bone sections were produced using the thin-section technique according to Donath, ultimately obtaining a section thickness of approximately 20 μm. Fifteen specimens were available for histomorphometry. The bone sections were digitized and analyzed. The bone-to-metal contact (BMC) parameter was determined histomorphometrically. The BMC was returned in terms of the visibly bone-covered implant surfaces as a percentage of the total implant surface shown.The values obtained for the six implants were arranged as six maximum-distance pairs and tested for significance using the t-test for dependent samples. The mean difference in BIC was 11.69±9.79%. The two-sided test showed a significant difference (p=0.033).The accidental or deliberate choice of section plane for a bone-implant block has an influence on the BIC value. To make BIC values more comparable, a standardization of section planes is desirable.

scholarly journals Assessment of the Tissue Response to Modification of the Surface of Dental Implants with Carboxyethylphosphonic Acid and Basic Fibroblastic Growth Factor Immobilization (Fgf-2): An Experimental Study on Minipigs

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 358
Author(s):  
Javier Aragoneses ◽  
Ana Suárez ◽  
Nansi López-Valverde ◽  
Francisco Martínez-Martínez ◽  
Juan Manuel Aragoneses
Keyword(s):  
Surface Treatment ◽  
Test Group ◽  
Mean Value ◽  
Control Group ◽  
P Value ◽  
Implant Surface ◽  
Bone Contact ◽  
Bone Implant ◽  
Significant Difference ◽  
The Mean

The aim of this study was to evaluate the effect of implant surface treatment with carboxyethylphosphonic acid and fibroblast growth factor 2 on the bone–implant interface during the osseointegration period in vivo using an animal model. The present research was carried out in six minipigs, in whose left tibia implants were inserted as follows: eight implants with a standard surface treatment, for the control group, and eight implants with a surface treatment of carboxyethylphosphonic acid and immobilization of FGF-2, for the test group. At 4 weeks after the insertion of the implants, the animals were sacrificed for the histomorphometric analysis of the samples. The means of the results for the implant–bone contact variable (BIC) were 46.39 ± 17.49% for the test group and 34.00 ± 9.92% for the control group; the difference was not statistically significant. For the corrected implant–bone contact variable (BICc), the mean value of the test group was 60.48 ± 18.11%, and that for the control group, 43.08 ± 10.77%; the difference was statistically significant (p-value = 0.035). The new bone formation (BV/TV) showed average results of 27.28 ± 3.88% for the test group and 26.63 ± 7.90% for the control group, meaning that the differences were not statistically significant (p-value = 0.839). Regarding the bone density at the interthread level (BAI/TA), the mean value of the test group was 32.27 ± 6.70%, and that of the control group was 32.91 ± 7.76%, with a p-value of 0.863, while for the peri-implant density (BAP/TA), the mean value of the test group was 44.96 ± 7.55%, and that for the control group was 44.80 ± 8.68%, without a significant difference between the groups. The current research only found a significant difference for the bone–implant contact at the cortical level; therefore, it could be considered that FGF-2 acts on the mineralization of bone tissue. The application of carboxyethylphosphonic acid on the surface of implants can be considered a promising alternative as a biomimetic coating for the immobilization of FGF-2. Despite no differences in the new bone formation around the implants or in the interthread or peri-implant bone density being detected, the biofunctionalization of the implant surface with FGF-2 accelerates the mineralization of the bone–implant interface at the cortical level, thereby reducing the osseointegration period.

scholarly journals Differences of Post-Placement Bone Implant Contact (BIC) Value of Dental Implant Coated and Not Coated With Platelet Rich Plasma (PRP)

2017 ◽  
Vol 10 (2) ◽  
pp. 11
Author(s):  
Bahruddin Thalib ◽  
Edy Machmud ◽  
Mochammad Dharmautama ◽  
Ervina Sari Surya ◽  
Asmawati Asmawati ◽  
...  
Keyword(s):  
Dental Implant ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Bone Implant ◽  
Significant Difference ◽  
Experimental Laboratory ◽  
The Mean ◽  
And Control ◽  
Veterinary Faculty ◽  
Implant Treatment

OBJECTIVE: The success of a dental dental implant treatment focuses on a phenomenon called osseointegration. Evaluation of Bone Area (BA) and Bone-Dental implant Contact (BIC) through histomorphometric analysis is the most widely used parameter to measure osseointegration. The aim of this study was to see post-placement Bone Dental implant Contact (BIC) value of dental implant coated and not coated with PRP.MATERIALS & METHODS: This study was an experimental laboratory conducted at Laboratory of Veterinary Faculty, Hasanuddin University. The sample was baby buck rabbit, aged 4-8 months old, weight 1500–2000 gram, divided into 2 groups each group consist of 12 rabbit, control group not coated with PRP and treatment group coated with PRP. Data analysis using SPSS version 20.0 (SPSS Inc., Chicago, IL, USA).RESULTS: There was a significant difference between the mean BIC values and the 20% increase in BIC values in LP1, LP2 and LP3 between treatment and control group on day 0, 3, 7, and 14.CONCLUSIONS: There was a difference in the average of post-placement BIC value of dental implant coated and not coated with PRP.

Histologic and Radiographic Evaluation of Progressive Loading on Implants Placed into Extraction Sockets

2007 ◽  
Vol 330-332 ◽  
pp. 1413-1416 ◽  
Author(s):  
Yu Ban ◽  
Ping Gong ◽  
Shao An Wang ◽  
N. Geng ◽  
X.G. Liu ◽  
...  
Keyword(s):  
Bone Loss ◽  
Radiographic Evaluation ◽  
Contact Ratio ◽  
Control Groups ◽  
Bone Implant ◽  
Paired Student ◽  
Loading System ◽  
Bone To Implant Contact ◽  
The Mean ◽  
Progressive Loading

To evaluate the effects of progressive loading on implants placed into extraction sockets. 4 beagle dogs were extracted bilateral 3rd, 4th and 2nd premolar and immediately inserted the implant at first surgery (0d), 14d and 21d. Using the newly developed vertical occlusion loading system, progressive loading procedures were taken 24h after insertion. The vertical bone loss (VBL) and bone-to-implant contact ratio (BIC) was measured by image analysis software and analyzed using the paired student t test. After 28d period of healing, each animal was sacrificed and the samples were evaluated by scanning electron microscopy (SEM). The mean vertical bone loss in control groups was larger than that of the experimental groups at 14, 21 and 28 day (P<0.05). SEM showed that the mineralization rate of experimental groups was faster than the control groups. The bone-implant contact ratios in experimental groups were more than that in the control groups. The differences were statistically significant (P<0.05). Compared with non-loading implant, progressive loading can promote osseointegration and decrease vertical bone loss. Progressive loading of implants placed into extraction sockets can be performed without interfering with the osseointegration process.

Impact of Smoking on Human Bone Apposition at Different Dental Implant Surfaces: A Histologic Study in Type IV Bone

2010 ◽  
Vol 36 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Susana d'Avila ◽  
Leonardo Delfino dos Reis ◽  
Adriano Piattelli ◽  
Kelly C. S. Aguiar ◽  
Marcelo de Faveri ◽  
...  
Keyword(s):  
Healing Time ◽  
Machined Surface ◽  
Implant Surface ◽  
Bone Apposition ◽  
Type Iv ◽  
Posterior Maxilla ◽  
Bone To Implant Contact ◽  
The Mean ◽  
Edentulous Patients ◽  
Etched Surface

Abstract Smoking has adverse effects on peri-implant bone healing and can cause bone loss around successfully integrated implants placed on type IV bone. This study evaluated the influence of implant surface topography of microimplants retrieved from posterior maxilla of smokers after 2 months of unloaded healing. Seven partially edentulous patients received 2 microimplants (machined and sandblasted acid-etched surface) each during conventional implant surgery. Histometric evaluation showed that the mean bone to implant contact was 10.40 ± 14.16% and 22.19 ± 14.68% to machined and sandblasted acid-etched surfaces, respectively (P &lt; .001). These data suggest that the sandblasted acid-etched surface presented better results than the machined surface after a short healing time in smokers.

scholarly journals Effect of PTH and corticotomy on implant movement under mechanical force

2020 ◽  
Author(s):  
Jiyeon Kim ◽  
Heon-Young Kim ◽  
Won-Ho Kim ◽  
Jin-Woo Kim ◽  
Minji Kim
Keyword(s):  
Parathyroid Hormone ◽  
Bone Remodeling ◽  
Traction Force ◽  
Mechanical Force ◽  
High Dose ◽  
Histomorphometric Analysis ◽  
Implant Surface ◽  
Bone Implant ◽  
Normal Dose ◽  
Osseointegrated Implants

Abstract BackgroundOsseointegrated implants are considered as clinically non-movable. Parathyroid hormone (PTH) is known to play a significant role in the regulation of bone remodeling and in intermittent, low doses, result in osteoanabolic effects. This study aimed to investigate the effects of PTH and corticotomy, both under traction force, on osseointegrated implants.MethodsFour implants—two in each hemimandible—were placed in each of the three study mongrels. Each mongrels were designated as control, normal dose PTH (PTH-1), and high dose PTH (PTH-2) groups, with each groups further subdivided into non-surgery implant and surgery implant. After osseointegration, mechanical force with NiTi closed coil springs (500g) was applied around each implants. Corticotomy was performed around one of four implants in each mongrels. Parathyroid hormone was administered locally on a weekly basis for 20 weeks. Clinical movement of the implants were evaluated with the superimposed 3D- scanned data, bone- microarchitectural and histologic examinations.ResultsSuperimposition analysis showed continuous movement of the non-surgery implant of PTH-1 group. Movement was further justified with lowest bone implant contact (adjusted BIC; 44.77%) in histomorphometric analysis. Upregulation of bone remodeling around the implant was observed in the normal dose PTH group. In the surgery implants, the remarkably higher adjusted BIC compared to the non-surgery implants indicated increased bone formation around the implant surface. ConclusionThe results indicate that the catabolic and anabolic balance of osseointegrated implants in terms of bone remodeling can be shifted via various interventions including pharmacological, surgical and mechanical force.Clinical RelevanceUpregulated bone remodeling by PTH and corticotomy under continuous mechanical force showed the possible implications for the movement of osseointegrated dental implant.

scholarly journals ANALYSIS OF BONE IMPLANT TO CONTACT VALUE USING SCANNING ELECTRON MICROSCOPE IN POST IMMEDIATE IMPLANTS WITH ADDITION OF GEL-EXTRACTED ROSELLA FLOWER

2019 ◽  
pp. 234-236
Author(s):  
MOHAMMAD DHARMAUTAMA ◽  
BAHRUDDIN THALIB ◽  
YULI SUSANIAWATY ◽  
IRFAN DAMMAR ◽  
ANDI ADYTHA MIR
Keyword(s):  
Bone Growth ◽  
Mean Value ◽  
Control Group ◽  
Bone Implant ◽  
Flower Extract ◽  
Inflammatory Phase ◽  
Immediate Implants ◽  
Bone To Implant Contact ◽  
The Mean ◽  
The Difference

Objective: The objective of the study was to analyze the difference of bone to implant contact (BIC) value post-immediate implants with or withoutinjecting rosella flower extracts gel in mongrel.Methods: A total of 18 implants were inserted in 9 mongrel’s dog. The implant was divided into two groups. The first one (test implant) was implantedimmediately after injection of gel-extracted rosella flower 10%, while another group (control implant) was implanted immediately after the toothextraction. The histomorphometry analysis was done after 14, 28, and 56 days to find out the BIC value.Result: The mean value of BIC rosella group was 63.44% and the mean value of the control group was 52.11%, with p=0.041.Conclusion: Rosella flower extract is able to shorten the inflammatory phase after implantation, accelerate and expand bone growth by spurringcollagen deposition as well as inhibiting osteoclast activity.

scholarly journals INFLUÊNCIA DA DISTÂNCIA NA EXATIDÃO DA CUBAGEM NÃO DESTRUTIVA DE QUALEA SP. NO BIOMA AMAZÔNIA

Nativa ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 663-670
Author(s):  
Rafaella De Angeli Curto ◽  
Arthur Faganello Teodoro Dos Anjos ◽  
Emanuel José Gomes De Araújo ◽  
Charlote Wink ◽  
Sintia Valerio Kohler
Keyword(s):  
Residual Analysis ◽  
T Test ◽  
Mean Values ◽  
Significant Difference ◽  
Standing Trees ◽  
The Mean ◽  
Dependent Samples ◽  
Non Destructive

Objetivou-se avaliar o efeito da distância na exatidão do dendrômetro digital Criterion RD 1000®, na estimativa do diâmetro e volume de Qualea sp. no bioma Amazônia. Foram selecionadas 30 árvores e cubadas pelo método de Smalian até a altura comercial com escalada e com Criterion RD 1000®, nas distâncias entre observador e a árvore de 11 m, 13 m, 15 m e à maior distância que o observador conseguiu instalar o equipamento em relação a árvore. Para avaliar o diâmetro ao longo do fuste, o volume comercial e por segmento, realizou-se o teste t pareado, com 95% de probabilidade, a análise de resíduos e as estatísticas complementares. Não houve diferença significativa entre os valores médios das variáveis analisadas, obtidos com a escalada e os estimados pelo Criterion. Houve menor amplitude dos resíduos nas seções inferiores do fuste com tendência em subestimar os menores diâmetros. As estatísticas complementares demonstraram maiores erros quando o equipamento estava mais próximo da árvore e menores quando estava a 15 m, correspondendo a uma distância superior à média da altura comercial das árvores. O equipamento permitiu estimar com exatidão diâmetros e volumes de árvores em pé, viabilizando a cubagem não destrutiva de Qualea sp. no bioma Amazônia.  Palavras-chave: mensuração; volumetria; Criterion RD 1000®.   INFLUENCE OF DISTANCE ON THE ACCURACY OF THE NON-DESTRUCTIVE OF Qualea sp. IN THE AMAZON BIOME   ABSTRACT: The objective was to evaluate the effect of distance on the accuracy of the digital dendrometer Criterion RD 1000®, on the estimate of the diameter and volume of Qualea sp. in the Amazon biome. Thirty trees were selected and scaled by the Smalian method to commercial height with climbing and with a Criterion RD 1000®, in the distances between the observer and the 11 m, 13 m, 15 m and the longest distance that the observer managed to install the equipment in relation to the tree. To analyze diameter along the stem, commercial volume and volume per segment, the t test for dependent samples was performed, with a 95% probability, as well as residual analysis and complementary statistics. There was no significant difference between the mean values ​​of the variables analyzed, obtained with the climb and estimated with a Criterion. There was a smaller amplitude of residues in the lower sections of the stem with tendency to underestimate smaller diameters. The complementary statistics showed greater errors with the equipment closest to the tree and smaller when it was 15 m away, corresponding to a distance greater than the average commercial height of the trees. The equipment made it possible to accurately estimate the diameters and volumes of standing trees, enabling the non-destructive scaling of Qualea sp. in the Amazon biome forest. Keywords: measurement; volumetry; Criterion RD 1000®.

scholarly journals Effect of PTH and corticotomy on implant movement under mechanical force

2020 ◽  
Author(s):  
Jiyeon Kim ◽  
Heon-Young Kim ◽  
Won-Ho Kim ◽  
Jin-Woo Kim ◽  
Minji Kim
Keyword(s):  
Parathyroid Hormone ◽  
Bone Remodeling ◽  
Traction Force ◽  
Mechanical Force ◽  
High Dose ◽  
Histomorphometric Analysis ◽  
Implant Surface ◽  
Bone Implant ◽  
Normal Dose ◽  
Osseointegrated Implants

Abstract Background Osseointegrated implants are considered as clinically non-movable. Parathyroid hormone (PTH) is known to play a significant role in the regulation of bone remodeling and in intermittent, low doses, result in osteoanabolic effects. This study aimed to investigate the effects of PTH and corticotomy, both under traction force, on osseointegrated implants.MethodsFour implants—two in each hemimandible—were placed in each of the three study mongrels. Each mongrels were designated as control, normal dose PTH (PTH-1), and high dose PTH (PTH-2) groups, with each groups further subdivided into non-surgery implant and surgery implant. After osseointegration, mechanical force with NiTi closed coil springs (500g) was applied around each implants. Corticotomy was performed around one of four implants in each mongrels. Parathyroid hormone was administered locally on a weekly basis for 20 weeks. Clinical movement of the implants were evaluated with the superimposed 3D- scanned data, bone- microarchitectural and histologic examinations. ResultsSuperimposition analysis showed continuous movement of the non-surgery implant of PTH-1 group. Movement was further justified with lowest bone implant contact (adjusted BIC; 44.77%) in histomorphometric analysis. Upregulation of bone remodeling around the implant was observed in the normal dose PTH group. In the surgery implants, the remarkably higher adjusted BIC compared to the non-surgery implants indicated increased bone formation around the implant surface. ConclusionThe results indicate that the catabolic and anabolic balance of osseointegrated implants in terms of bone remodeling can be shifted via various interventions including pharmacological, surgical and mechanical force. Clinical RelevanceUpregulated bone remodeling by PTH and corticotomy under continuous mechanical force showed the possible implications for the movement of osseointegrated dental implant.

The Effect of Constructivist Teaching Approach on the Conceptual Understanding of Biology Students

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Marlyn D. Ronsairo
Keyword(s):  
Conceptual Understanding ◽  
Marine Biology ◽  
Teaching Approach ◽  
Constructivist Teaching ◽  
Cognitive Level ◽  
Biology Students ◽  
Significant Difference ◽  
Other Information ◽  
The Mean ◽  
Dependent Samples

This study determines the effect of constructivist-based teaching approach on the cognitive and affective levels of conceptual understanding of Marine Biology of BS Biology students. The study made use of the pre-test-posttest one-shot experimental design. The participants were selected using purposive sampling. Data and other information needed in this study were gathered mainly through conceptual understanding test, formal interviews and journals. And to treat these data, the mean scoring system and t-test for dependent samples were used. Results revealed that the extent of cognitive level of conceptual understanding in Marine Biology of the students based on their journals was observed to a very great extent. On the other hand, the extent of affective level of conceptual understanding of the students in Marine Biology as revealed in their journals was observed to a great extent. Moreover, the findings also showed that there is significant difference on the students’ level of conceptual understanding based on their mean scores in the test after using the constructivist-based teaching approach. Lastly, it is also found out that the students learned and appreciated Marine Biology through constructivist-based teaching approach based on the interviews conducted to them.   Keywords - conceptual understanding, constructivist-based teaching approach

Human Histologic Evaluation of a Six-year-old Threaded Implant Retrieved from a Subject with Osteoporosis

2008 ◽  
Vol 9 (3) ◽  
pp. 99-105 ◽  
Author(s):  
Adriano Piattelli ◽  
Jamil A. Shibli ◽  
Leandro de Melo ◽  
Giovanna Lezzi ◽  
Susana d'Avila ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Close Contact ◽  
Human Life ◽  
Elderly Subjects ◽  
Implant Surface ◽  
Implant Placement ◽  
Histologic Evaluation ◽  
Bone To Implant Contact ◽  
The Mean ◽  
Cement Lines

Abstract Aim The aim of this case report was to present the evaluation of the bone-to-implant contact in an implant retrieved from a subject with osteoporosis after six years of load. Background Systemic conditions associated with osteoporosis have been postulated to contribute to the severity of alveolar bone loss. The increase in human life expectancy, the increased number of elderly subjects who are partially or totally edentulous, and the use of dental implants for oral habilitation in subjects with osteoporosis has raised several questions. Report A 68-year-old woman with postmenopausal osteoporosis received a prosthetic evaluation of an implant-supported restoration. Histologically, the peri-implant bone appeared healthy. The peri-implant bone appeared in close contact with the implant surface, whereas marrow spaces could be detected in other areas along with prominently stained cement lines. The mean of bone-to-implant contact was 62.51±1.96. Conclusion The results of the evaluation of the dental implant reported here suggest the presence of osteoporosis may not be a contra-indication for implant placement at least after osseointegration has already been established. Citation de Melo L, Piattelli A, Lezzi G, d'Avila S, Zenóbio EG, Shibli JA. Human Histologic Evaluation of a Six-year-old Threaded Implant Retrieved from a Subject with Osteoporosis. J Contemp Dent Pract 2008 March; (9)3:099-105.

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