scholarly journals Solution NMR studies of periplasmic binding proteins and their interaction partners

2011 ◽  
Vol 2 (1-2) ◽  
pp. 53-64 ◽  
Author(s):  
Sara Pistolesi ◽  
Nico Tjandra ◽  
Guillermo A. Bermejo
Keyword(s):  
Large Scale ◽  
Binding Proteins ◽  
Basic Research ◽  
Paramagnetic Nmr ◽  
Nmr Studies ◽  
Practical Applications ◽  
Periplasmic Binding Proteins ◽  
Interaction Partners ◽  
Allosteric Mechanisms ◽  
Crucial Part

AbstractPeriplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed-liganded, which signals the presence of substrate and starts its translocation. Over the years, PBPs have been extensively studied not only owing to their essential role in nutrient uptake but also because they serve as excellent models for both practical applications (e.g., biosensor technology) and basic research (e.g., allosteric mechanisms). Although much of our knowledge at atomic level has been inferred from the detailed, static pictures afforded by crystallographic studies, nuclear magnetic resonance (NMR) has been able to fill certain gaps in such body of work, particularly with regard to dynamic processes. Here, we review NMR studies on PBPs, and their unique insights on conformation, dynamics, energetics, substrate binding, and interactions with related transport proteins. Based on the analysis of recent paramagnetic NMR results, as well as crystallographic and functional observations, we propose a mechanism that could explain the ability of certain PBPs to achieve a closed conformation in absence of ligand while others seem to remain open until ligand-mediated closure.

scholarly journals S1f2-1 NMR studies of periplasmic binding proteins(S1-f2: "Functions and dynamics of protein systems in various aspect",Symposia,Abstract,Meeting Program of EABS & BSJ 2006)

2006 ◽  
Vol 46 (supplement2) ◽  
pp. S119
Author(s):  
Yutaka Ito ◽  
Kaori Kurashima-Ito ◽  
Kayano Moromisato ◽  
Takao Inoue ◽  
Kaoru Nishimura ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Nmr Studies ◽  
Meeting Program ◽  
Periplasmic Binding Proteins

Bullying, School Violence, and Climate in Evolving Contexts

2018 ◽  
Author(s):  
Ron Avi Astor ◽  
Rami Benbenisthty
Keyword(s):  
School Violence ◽  
School Safety ◽  
Media Coverage ◽  
Large Scale ◽  
Ecological Model ◽  
Empirical Studies ◽  
Practical Applications ◽  
Teacher Student ◽  
Area Of Interest ◽  
New Research

Since 2005, the bullying, school violence, and school safety literatures have expanded dramatically in content, disciplines, and empirical studies. However, with this massive expansion of research, there is also a surprising lack of theoretical and empirical direction to guide efforts on how to advance our basic science and practical applications of this growing scientific area of interest. Parallel to this surge in interest, cultural norms, media coverage, and policies to address school safety and bullying have evolved at a remarkably quick pace over the past 13 years. For example, behaviors and populations that just a decade ago were not included in the school violence, bullying, and school safety discourse are now accepted areas of inquiry. These include, for instance, cyberbullying, sexting, social media shaming, teacher–student and student–teacher bullying, sexual harassment and assault, homicide, and suicide. Populations in schools not previously explored, such as lesbian, gay, bisexual, transgender, and queer students and educators and military- and veteran-connected students, become the foci of new research, policies, and programs. As a result, all US states and most industrialized countries now have a complex quilt of new school safety and bullying legislation and policies. Large-scale research and intervention funding programs are often linked to these policies. This book suggests an empirically driven unifying model that brings together these previously distinct literatures. This book presents an ecological model of school violence, bullying, and safety in evolving contexts that integrates all we have learned in the 13 years, and suggests ways to move forward.

scholarly journals 2D Nanomaterials for Effective Energy Scavenging

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Md Al Mahadi Hasan ◽  
Yuanhao Wang ◽  
Chris R. Bowen ◽  
Ya Yang
Keyword(s):  
Large Scale ◽  
Waste Heat ◽  
Material Selection ◽  
Power Supplies ◽  
Small Scale ◽  
Energy Scavenging ◽  
Power Sources ◽  
Practical Applications ◽  
2D Nanomaterials ◽  
Self Powered

AbstractThe development of a nation is deeply related to its energy consumption. 2D nanomaterials have become a spotlight for energy harvesting applications from the small-scale of low-power electronics to a large-scale for industry-level applications, such as self-powered sensor devices, environmental monitoring, and large-scale power generation. Scientists from around the world are working to utilize their engrossing properties to overcome the challenges in material selection and fabrication technologies for compact energy scavenging devices to replace batteries and traditional power sources. In this review, the variety of techniques for scavenging energies from sustainable sources such as solar, air, waste heat, and surrounding mechanical forces are discussed that exploit the fascinating properties of 2D nanomaterials. In addition, practical applications of these fabricated power generating devices and their performance as an alternative to conventional power supplies are discussed with the future pertinence to solve the energy problems in various fields and applications.

scholarly journals Application of Novel Non-Thermal Physical Technologies to Degrade Mycotoxins

2021 ◽  
Vol 7 (5) ◽  
pp. 395
Author(s):  
Mohammad Yousefi ◽  
Masoud Aman Mohammadi ◽  
Maryam Zabihzadeh Khajavi ◽  
Ali Ehsani ◽  
Vladimír Scholtz
Keyword(s):  
Electron Beam ◽  
Food Systems ◽  
Large Scale ◽  
Plasma Electron ◽  
Pulsed Light ◽  
Beam Radiation ◽  
Practical Applications ◽  
Electron Beam Radiation ◽  
Non Thermal Plasma ◽  
Agriculture And Food

Mycotoxins cause adverse effects on human health. Therefore, it is of the utmost importance to confront them, particularly in agriculture and food systems. Non-thermal plasma, electron beam radiation, and pulsed light are possible novel non-thermal technologies offering promising results in degrading mycotoxins with potential for practical applications. In this paper, the available publications are reviewed—some of them report efficiency of more than 90%, sometimes almost 100%. The mechanisms of action, advantages, efficacy, limitations, and undesirable effects are reviewed and discussed. The first foretastes of plasma and electron beam application in the industry are in the developing stages, while pulsed light has not been employed in large-scale application yet.

scholarly journals A Short Review on Recent Advances of Hydrogel-Based Adsorbents for Heavy Metal Ions

Metals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 864
Author(s):  
Suguna Perumal ◽  
Raji Atchudan ◽  
Thomas Nesakumar Jebakumar Immanuel Edison ◽  
Rajendran Suresh Babu ◽  
Petchimuthu Karpagavinayagam ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Large Scale ◽  
Water Bodies ◽  
Dimensional Structure ◽  
High Porosity ◽  
Adsorption Method ◽  
Toxic Heavy Metal ◽  
Practical Applications

The growth of industry fulfills our necessity and promotes economic development. However, pollutants from such industries pollute water bodies which pose a high risk for living organisms. Thus, researchers have been urged to develop an efficient method to remove toxic heavy metal ions from water bodies. The adsorption method shows promising results for the removal of heavy metal ions and is easy to operate on a large scale, thus can be applied to practical applications. Numerous adsorbents were developed and reported, among them hydrogels, which attract great attention because of the reusability, ease of preparation, and handling. Hydrogels are generally prepared by the cross-linking of polymers that result in a three-dimensional structure, showing high porosity and high functionality. They are hydrophilic in nature because of the functional groups, and are non-toxic. Thus, this review provides various methods of hydrogel adsorbents preparation and summarizes recent progress in the use of hydrogel adsorbents for the removal of heavy metal ions. Further, the mechanism involved in the removal of heavy metal ions is briefly discussed. The most recent studies about the adsorption method for the treatment of heavy metal ions contaminated water are presented.

scholarly journals Blockchain-Based Application for Certification Management

2020 ◽  
Vol 14 (4) ◽  
pp. 488-492
Author(s):  
Jovan Karamachoski ◽  
Ninoslav Marina ◽  
Pavel Taskov
Keyword(s):  
Supply Chain ◽  
Business Models ◽  
Human Factor ◽  
Computer Code ◽  
Chain Management ◽  
Practical Applications ◽  
Sustainable Technologies ◽  
Blockchain Technology ◽  
Smart Contract ◽  
Crucial Part

Blockchain technology will bring a disruption in plenty of industries and businesses. Recently it proved the robustness, immutability, auditability, in many crucial practical applications. The blockchain structure offers traceability of actions, alterations, alerts, which is an important property of a system needed for development of sustainable technologies. A crucial part of the blockchain technology regarding the optimization of the processes is the smart contract. It is a self-executable computer code, open and transparent, encoding the terms of a regular contract. It is able to automate the processes, thus decreasing the human-factor mistakes or counterfeits. In this paper, we are presenting the feasibility of the blockchain technology in the certification processes, with an application developed for university diploma certification. The example is easily transferable in other areas and business models such as logistics, supply chain management, or other segments where certification is essential.

scholarly journals Predicting AC Optimal Power Flows: Combining Deep Learning and Lagrangian Dual Methods

2020 ◽  
Vol 34 (01) ◽  
pp. 630-637 ◽  
Author(s):  
Ferdinando Fioretto ◽  
Terrence W.K. Mak ◽  
Pascal Van Hentenryck
Keyword(s):  
Deep Learning ◽  
Power Systems ◽  
Power Flow ◽  
Optimal Power Flow ◽  
Large Scale ◽  
Renewable Energy Sources ◽  
Electrical Power ◽  
Practical Applications ◽  
Fundamental Building Block ◽  
Optimal Power

The Optimal Power Flow (OPF) problem is a fundamental building block for the optimization of electrical power systems. It is nonlinear and nonconvex and computes the generator setpoints for power and voltage, given a set of load demands. It is often solved repeatedly under various conditions, either in real-time or in large-scale studies. This need is further exacerbated by the increasing stochasticity of power systems due to renewable energy sources in front and behind the meter. To address these challenges, this paper presents a deep learning approach to the OPF. The learning model exploits the information available in the similar states of the system (which is commonly available in practical applications), as well as a dual Lagrangian method to satisfy the physical and engineering constraints present in the OPF. The proposed model is evaluated on a large collection of realistic medium-sized power systems. The experimental results show that its predictions are highly accurate with average errors as low as 0.2%. Additionally, the proposed approach is shown to improve the accuracy of the widely adopted linear DC approximation by at least two orders of magnitude.

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