Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity

2006 ◽  
Vol 387 (12) ◽  
Author(s):  
Lenka Grunclová ◽  
Martin Horn ◽  
Marie Vancová ◽  
Daniel Sojka ◽  
Zdeněk Franta ◽  
...  
Keyword(s):  
Differential Expression ◽  
Expression Pattern ◽  
Soft Tick ◽  
Inhibitory Specificity ◽  
Ornithodoros Moubata ◽  
Differential Expression Pattern

scholarly journals Differential Expression Pattern of C4 Bundle Sheath Expression Genes in Rice, a C3 Plant

2005 ◽  
Vol 46 (5) ◽  
pp. 754-761 ◽  
Author(s):  
Mika Nomura ◽  
Tomonori Higuchi ◽  
Yuji Ishida ◽  
Shozo Ohta ◽  
Toshihiko Komari ◽  
...  
Keyword(s):  
Differential Expression ◽  
Expression Pattern ◽  
Bundle Sheath ◽  
C3 Plant ◽  
Differential Expression Pattern

scholarly journals Differential Expression Pattern of Retinoid X Receptors in Adult Murine Testicular Cells Implies Varying Roles for these Receptors in Spermatogenesis1

1998 ◽  
Vol 58 (6) ◽  
pp. 1351-1356 ◽  
Author(s):  
Ingrid C. Gaemers ◽  
Ans M.M. van Pelt ◽  
Paul T. van der Saag ◽  
Jos W. Hoogerbrugge ◽  
Axel P.N. Themmen ◽  
...  
Keyword(s):  
Differential Expression ◽  
Expression Pattern ◽  
Retinoid X Receptors ◽  
Testicular Cells ◽  
Differential Expression Pattern ◽  
X Receptors

scholarly journals Epigenetic Modification as a Regulatory Mechanism for Spatiotemporal Dynamics of ANO1 Expression in Salivary Glands

2019 ◽  
Vol 20 (24) ◽  
pp. 6298 ◽  
Author(s):  
Yonghwan Shin ◽  
Sang-Woo Lee ◽  
Eun Namkoong ◽  
Woojin An ◽  
Jong-Ho Lee ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Differential Expression ◽  
Expression Pattern ◽  
Dna Methyltransferase ◽  
Epigenetic Modification ◽  
Critical Role ◽  
Cpg Islands ◽  
Ductal Cells ◽  
Differential Expression Pattern

Anoctamin1 (ANO1), a calcium activated chloride channel, is known to play a critical role in salivary secretion. In the salivary gland, ANO1 is expressed exclusively in the acinar cells, with no expression in the ductal cells. However, the mechanisms that determine this distinctive cell type-dependent expression pattern of ANO1 remain unknown. In this study, we discovered that the cell-dependent expression of ANO1 during salivary gland organogenesis is regulated by DNA methylation of ANO1 CpG islands. ANO1 CpG islands in e12 embryonic submandibular glands (eSMG) are highly methylated, but those in e14 eSMG or adult SMG are significantly unmethylated. The differential expression pattern of ANO1 in duct and acini is defined at e14. Artificial demethylation by treatment with the demethylating agent 5-aza-2’-deoxycytidine (5-Aza-CdR), induced the expression of ANO1 in both the ductal cell line Human Submandibular Gland (HSG) and in the duct cells of adult mouse SMG. During the trans-differentiation in Matrigel of duct-origin HSG cells into acinar-like phenotype, significant demethylation of ANO1 CpG islands is observed. This may be due to the reduced expression of DNA methyltransferase (DNMT) 3a and 3b. These results suggest that the differential expression of ANO1 in salivary glands during organogenesis and differentiation is mainly regulated by epigenetic demethylation of the ANO1 gene.

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