The chemistry of nitrosative stress induced by nitric oxide and reactive nitrogen oxide species. Putting perspective on stressful biological situations

2004 ◽  
Vol 385 (1) ◽  
pp. 1-10 ◽  
Author(s):  
L. A. Ridnour ◽  
D. D. Thomas ◽  
D. Mancardi ◽  
M. G. Espey ◽  
K. M. Miranda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitrogen Oxide ◽  
Nitrosative Stress ◽  
Reactive Nitrogen ◽  
Reactive Nitrogen Oxide Species

Inhibition of poly(ADP-RIBOSE) polymerase (PARP) by nitric oxide and reactive nitrogen oxide species

2003 ◽  
Vol 35 (11) ◽  
pp. 1431-1438 ◽  
Author(s):  
Olga Sidorkina ◽  
Michael Graham Espey ◽  
Katrina M Miranda ◽  
David A Wink ◽  
Jacques Laval
Keyword(s):  
Nitric Oxide ◽  
Nitrogen Oxide ◽  
Reactive Nitrogen ◽  
Reactive Nitrogen Oxide Species

The content of nitric oxide and S-nitrosothiols in rats’ liver cells under the different supplementation of macronutrient

2020 ◽  
Vol 12 (2) ◽  
pp. 187-195
Author(s):  
Halyna Kopylchuk ◽  
Ivanna Nykolaichuk ◽  
Olesiia Kuziak
Keyword(s):  
Nitric Oxide ◽  
Nitrogen Oxide ◽  
Nitrosative Stress ◽  
Liver Cells ◽  
Oxide Content ◽  
Experimental Conditions ◽  
Before And After ◽  
Rat Liver Cells ◽  
Key Factor ◽  
Excessive Consumption

This paper presents studies of nitric oxide and low-molecular S-nitrosothiols in the mitochondrial and cytosolic fractions of the rats' liver under the conditions of, alimentary protein deprivation, consumption of excess sucrose content and combined action of two adverse factors. In order to model the low-protein diet of the animal for 28 days received an isocaloric diet containing 4.7% protein, 10% fat, 81,3% carbohydrates (starch – 37%, sucrose – 30%, cellulose – 5%) and was calculated in accordance with the recommendations of the American Institute of Nutrition. The high-sugar diet consisted of 14% protein, 10% fat, 72% carbohydrates (starch – 37%, sucrose – 30%, cellulose – 5%). The mitochondrial and cytosolic fraction of rat liver cells were obtained by the method of differential centrifugation. Nitrogen oxide content was assessed by a unified method by determining the NO2- content, which is a stable metabolite of nitric oxide. Since NO is inactivated into an oxidase reaction with the conversion into nitrite or nitrate that is quickly metabolized, the nitrogen oxide content was assessed by the change in NO2-. The concentration of S-nitrosothiols was recorded, respectively, by determining the concentration of nitrite anion before and after the addition of Hg2+ ions, which by modifying the S – N bonds catalyzes the release of S-nitrosyl thiols of nitric oxide. An increase in NO content in both hepatic subcellular fractions of the rats’ experimental groups compared to control values was found. However, a lack of protein in the diet (protein deficiency in the diet leads to an increase in nitric oxide levels in 3-4 times) can be considered as a key factor in the recorded changes in the mitochondria of the animals’ liver, while in the cytosol - excessive consumption of sucrose (3-5 times increase). Regarding the level of S-nitrosothiols, in the studied fractions, multidirectional changes in their concentration were found. Thus, an increase in the content of nitrosyl derivatives in the mitochondria of rat’s liver cells with a simultaneous decrease in their level in the cytosol indicates dysmetabolic disorders in the transport system and deposition of nitric oxide, which can lead to the development of nitrosative stress under the experimental conditions.

scholarly journals Evidence for the nitration of γ-tocopherol in vivo: 5-nitro-γ-tocopherol is elevated in the plasma of subjects with coronary heart disease

2002 ◽  
Vol 364 (3) ◽  
pp. 625-628 ◽  
Author(s):  
Lincoln W. MORTON ◽  
Natalie C. WARD ◽  
Kevin D. CROFT ◽  
Ian B. PUDDEY
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Liquid Chromatography ◽  
Carotid Artery ◽  
Nitrogen Oxide ◽  
Atherosclerotic Plaque ◽  
Reactive Nitrogen ◽  
Tandem Ms ◽  
Reactive Nitrogen Oxide Species

This study investigated the hypothesis that nitration of γ-tocopherol may be an important mechanism for the detoxification of reactive nitrogen oxide species in vivo. Using liquid chromatography—tandem MS we have shown that γ-tocopherol can be nitrated in vivo to form 5-nitro-γ-tocopherol and that concentrations of this compound are elevated in the plasma of subjects with coronary heart disease. In addition, we demonstrate in carotid-artery atherosclerotic plaque that nitration of γ-tocopherol is also evident at levels similar to that seen in the plasma of subjects with coronary heart disease.

Determination of selectivity of reactive nitrogen oxide species for various substrates

1996 ◽  
pp. 120-130 ◽  
Author(s):  
David A. Wink ◽  
Matthew B. Grisham ◽  
Allen M. Miles ◽  
Raymond W. Nims ◽  
Murali C. Krishna ◽  
...  
Keyword(s):  
Nitrogen Oxide ◽  
Reactive Nitrogen ◽  
Reactive Nitrogen Oxide Species
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