Substrate Specificity and Inhibitor Studies of a Membrane-Bound Ganglioside Sialidase Isolated from Human Brain Tissue

2002 ◽  
Vol 383 (11) ◽  
Author(s):  
C. Oehler ◽  
J. Kopitz ◽  
M. Cantz
Keyword(s):  
Substrate Specificity ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Membrane Bound

scholarly journals RBIO-06. IMPLEMENTATION OF HUMAN INDUCED PLURIPOTENT STEM CELL DERIVED CEREBRAL ORGANOIDS TO MODEL NORMAL TISSUE RADIORESPONSE

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii193-ii193
Author(s):  
Lawrence Bronk ◽  
Sanjay Singh ◽  
Riya Thomas ◽  
Luke Parkitny ◽  
Mirjana Maletic-Savatic ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Tissue ◽  
Model Systems ◽  
Human Brain Tissue ◽  
Post Irradiation ◽  
Mature Neurons ◽  
Induced Pluripotent ◽  
Effects Of Radiation

Abstract Treatment-related sequelae following cranial irradiation have life changing impacts for patients and their caregivers. Characterization of the basic response of human brain tissue to irradiation has been difficult due to a lack of preclinical models. The direct study of human brain tissue in vitro is becoming possible due to advances in stem cell biology, neuroscience, and tissue engineering with the development of organoids as novel model systems which enable experimentation with human tissue models. We sought to establish a cerebral organoid (CO) model to study the radioresponse of normal human brain tissue. COs were grown using human induced pluripotent stem cells and a modified Lancaster protocol. Compositional analysis during development of the COs showed expected populations of neurons and glia. We confirmed a population of microglia-like cells within the model positive for the makers Iba1 and CD68. After 2-months of maturation, COs were irradiated to 0, 10, and 20 Gy using a Shepard Mark-II Cs-137 irradiator and returned to culture. Subsets of COs were prepared for immunostaining at 30- and 70-days post-irradiation. To examine the effect of irradiation on the neural stem cell (NSC) population, sections were stained for SOX2 and Ki-67 expression denoting NSCs and proliferation respectively. Slides were imaged and scored using the CellProfiler software package. The percentage of proliferating NSCs 30-days post-irradiation was found to be significantly reduced for irradiated COs (5.7% (P=0.007) and 3.4% (P=0.001) for 10 and 20 Gy respectively) compared to control (12.7%). The reduction in the proliferating NSC population subsequently translated to a reduced population of NeuN-labeled mature neurons 70 days post-irradiation. The loss of proliferating NSCs and subsequent reduction in mature neurons demonstrates the long-term effects of radiation. Our initial results indicate COs will be a valuable model to study the effects of radiation therapy on normal and diseased human tissue.

Demonstration of α1A-and α1B-adrenoceptor binding sites in human brain tissue

1989 ◽  
Vol 169 (2-3) ◽  
pp. 325-328 ◽  
Author(s):  
Gerhard Gross ◽  
Gertraud Hanft ◽  
Hubertus M. Mehdorn
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Binding Sites ◽  
Human Brain Tissue

The lipopigments in human brain tissue necroses

1980 ◽  
Vol 52 (2) ◽  
pp. 147-151 ◽  
Author(s):  
R. Schr�der ◽  
B. Reinartz
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue

Herpes Simplex Virus Type 1 DNA in Human Brain Tissue

1991 ◽  
pp. 53-66 ◽  
Author(s):  
J. Rajčáni ◽  
M. Murányiová ◽  
M. Kúdelová ◽  
J. Pogády ◽  
V. Mucha ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Human Brain ◽  
Brain Tissue ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Human Brain Tissue ◽  
Simplex Virus
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