Molecular Characterization of a Novel Mammalian DnaJ-Like Sec63p Homolog

1999 ◽  
Vol 380 (9) ◽  
pp. 1133-1138 ◽  
Author(s):  
Markus H. Skowronek ◽  
Max Rotter ◽  
Ingrid G. Haas
Keyword(s):  
Protein Translocation ◽  
Integral Membrane Protein ◽  
The Novel ◽  
Cell Extracts ◽  
Human Cdna Sequence ◽  
Specific Antisera ◽  
Rough Er ◽  
Sequence Encoding ◽  
Biochemical Analyses

AbstractWe identified a human cDNA sequence encoding a polypeptide of 760 amino acids that shares 53% homology and 25.6% identity with the yeast DnaJ-like endoplasmic reticulum (ER) translocon component Sec63p. Three epitope-specific antisera revealed a protein of an apparent molecular mass of 83 kDa, both in human cell extracts and in dog pancreatic microsomes. Biochemical analyses show that it is an integral membrane protein of the rough ER, which has the DnaJ domain located in the ER lumen. The novel Sec63 protein could thus represent a key component of the mammalian ER protein translocation machinery.

scholarly journals Characterization of Two Putative Protein Translocation Components in the Apicoplast of Plasmodium falciparum

2009 ◽  
Vol 8 (8) ◽  
pp. 1146-1154 ◽  
Author(s):  
Ming Kalanon ◽  
Christopher J. Tonkin ◽  
Geoffrey I. McFadden
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Trafficking ◽  
Protein Translocation ◽  
Protein A ◽  
Integral Membrane Protein ◽  
Diatom Algae ◽  
Trophozoite Stage ◽  
Replacement Strategy ◽  
Putative Protein

ABSTRACT Protein trafficking to the stroma of the apicoplast of Plasmodium falciparum requires translocation across several membranes. To further elucidate the mechanisms responsible, we investigated two proteins: P. falciparum Tic22 (PfTic22), a putative component of the translocon of the inner chloroplast membrane; and PfsDer1-1, one of two homologues of the P. falciparum symbiont-derived Der1 (sDer1) protein, a putative component of an endoplasmic reticulum-associated degradation (ERAD) complex in the periplastid membrane. We constructed parasites expressing hemagglutinin (HA)-tagged PfTic22 and PfsDer1-1 under the control of their endogenous promoters using the 3′ replacement strategy. We show that both PfTic22-HA and PfsDer1-1-HA are expressed predominantly during the trophozoite stage of the asexual replication cycle, which corresponds to the most dynamic stages of apicoplast activity. Although both proteins localize to the periphery of the apicoplast, PfTic22-HA is a membrane-associated protein while PfsDer1-1-HA is an integral membrane protein. Phylogenetic analysis indicates that PfsDer1-1 is one of two Der1 paralogues predicted to localize to the apicoplast in P. falciparum and that it has orthologues in diatom algae, supporting the chromalveolate hypothesis. These observations are consistent with putative roles for PfTic22 and PfsDer1-1 in protein translocation into the apicoplast of P. falciparum.

Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts

2011 ◽  
Vol 35 (5) ◽  
pp. 545-553 ◽  
Author(s):  
Supawadee Pata ◽  
Nualyai Yaraksa ◽  
Sakda Daduang ◽  
Yosapong Temsiripong ◽  
Jisnuson Svasti ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Antibacterial Peptide ◽  
The Novel ◽  
Cell Extracts ◽  
Crocodylus Siamensis

Characterization of the novel HLA‐C *07:944 allele by next‐generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Maria Loginova ◽  
Olga Makhova ◽  
Daria Smirnova ◽  
Igor Paramonov ◽  
Maksim Zarubin
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Generation Sequencing

Characterization of the novel HLA‐B *51:296 allele by next‐generation sequencing

HLA ◽  
2020 ◽  
Author(s):  
Steve Genebrier ◽  
Vincent Elsermans ◽  
Emeric Texeraud ◽  
Gerald Bertrand ◽  
Virginie Renac
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Generation Sequencing

Characterization of the novel HLA‐DRB1*11:282 allele by sequencing‐based typing

HLA ◽  
2021 ◽  
Author(s):  
Marine Cargou ◽  
Vincent Elsermans ◽  
Isabelle Top ◽  
Laura Blouin ◽  
Jonathan Visentin
Keyword(s):  
The Novel

scholarly journals Isolation and Characterization of Salmonella Jumbo-Phage pSal-SNUABM-04

Viruses ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 27
Author(s):  
Jun Kwon ◽  
Sang Guen Kim ◽  
Hyoun Joong Kim ◽  
Sib Sankar Giri ◽  
Sang Wha Kim ◽  
...  
Keyword(s):  
Morphological Traits ◽  
Open Reading Frames ◽  
Genome Comparison ◽  
The Novel ◽  
Promising Alternative ◽  
Isolation And Characterization ◽  
Global Issue ◽  
Reading Frames ◽  
Predicted Function

The increasing emergence of antimicrobial resistance has become a global issue. Therefore, many researchers have attempted to develop alternative antibiotics. One promising alternative is bacteriophage. In this study, we focused on a jumbo-phage infecting Salmonella isolated from exotic pet markets. Using a Salmonella strain isolated from reptiles as a host, we isolated and characterized the novel jumbo-bacteriophage pSal-SNUABM-04. This phage was investigated in terms of its morphology, host infectivity, growth and lysis kinetics, and genome. The phage was classified as Myoviridae based on its morphological traits and showed a comparatively wide host range. The lysis efficacy test showed that the phage can inhibit bacterial growth in the planktonic state. Genetic analysis revealed that the phage possesses a 239,626-base pair genome with 280 putative open reading frames, 76 of which have a predicted function and 195 of which have none. By genome comparison with other jumbo phages, the phage was designated as a novel member of Machinavirus composed of Erwnina phages.

Characterization of the novel HLA‐DQA1 *05:19 allele by next‐generation sequencing

HLA ◽  
2021 ◽  
Author(s):  
Steve Genebrier ◽  
Paul Rouzaire ◽  
Emeric Texeraud ◽  
Gerald Bertrand ◽  
Virginie Renac
Keyword(s):  
Next Generation Sequencing ◽  
The Novel ◽  
Next Generation ◽  
Hla Dqa1 ◽  
Generation Sequencing
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