Molecular Reaction Mechanisms of Proteins Monitored by Time-Resolved FTIR-Spectroscopy

1999 ◽  
Vol 380 (7-8) ◽  
Author(s):  
K. Gerwert
Keyword(s):  
Ftir Spectroscopy ◽  
Reaction Mechanisms ◽  
Time Resolved ◽  
Molecular Reaction

scholarly journals Partial Reactions of the Na,K-ATPase: Determination of Activation Energies and an Approach to Mechanism

2020 ◽  
Vol 253 (6) ◽  
pp. 631-645
Author(s):  
Hans-Jürgen Apell ◽  
Milena Roudna
Keyword(s):  
Reaction Mechanisms ◽  
Fluorescent Dyes ◽  
Activation Energies ◽  
Mechanistic Models ◽  
Time Resolved ◽  
Conformational Relaxation ◽  
Molecular Reaction ◽  
Rate Limiting ◽  
Induced Reaction

Abstract Kinetic experiments were performed with preparations of kidney Na,K-ATPase in isolated membrane fragments or reconstituted in vesicles to obtain information of the activation energies under turnover conditions and for selected partial reactions of the Post-Albers pump cycle. The ion transport activities were detected with potential or conformation sensitive fluorescent dyes in steady-state or time-resolved experiments. The activation energies were derived from Arrhenius plots of measurements in the temperature range between 5 °C and 37 °C. The results were used to elaborate indications of the respective underlying rate-limiting reaction steps and allowed conclusions to be drawn about possible molecular reaction mechanisms. The observed consequent alteration between ligand-induced reaction and conformational relaxation steps when the Na,K-ATPase performs the pump cycle, together with constraints set by thermodynamic principles, provided restrictions which have to be met when mechanistic models are proposed. A model meeting such requirements is presented for discussion. Graphic Abstract

scholarly journals Structural Changes Due to the Deprotonation of the Proton Release Group in the M-Photointermediate of Bacteriorhodopsin as Revealed by Time-Resolved FTIR Spectroscopy†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11598-11605 ◽  
Author(s):  
Joel E. Morgan ◽  
Ahmet S. Vakkasoglu ◽  
Johan Lugtenburg ◽  
Robert B. Gennis ◽  
Akio Maeda
Keyword(s):  
Ftir Spectroscopy ◽  
Structural Changes ◽  
Proton Release ◽  
Time Resolved ◽  
Release Group
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