scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oils from Black Pepper, Cumin, Coriander and Cardamom Against Some Pathogenic Microorganisms

2016 ◽  
Vol 20 (2) ◽  
pp. 39-52 ◽  
Author(s):  
Desislava Teneva ◽  
Zapryana Denkova ◽  
Bogdan Goranov ◽  
Rositsa Denkova ◽  
Georgi Kostov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Black Pepper ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Oil Composition ◽  
Gram Positive Bacteria ◽  
Inhibition Zones ◽  
Main Components

Abstract Four popular spices black pepper (Piper nigrum L.), cumin (Cuminum cyminum L.), coriander (Coriandrum sativum L.) and cardamom (Elettaria cardamomum) were analyzed for their oil composition by GC-MS. Thirty compounds were identified in the black pepper oil and the main components were β-caryophyllene (20.225 %), sabinene (18.054 %), limonene (16.924 %), α-pinene (9.171 %) and α-phellandrene (5.968 %). Twenty five compounds were identified in the cumin oil – cuminaldehyde (30.834 %), 3-caren-10-al (17.223 %), β-pinene (14.837 %), γ–terpinene (11.928 %), 2-caren-10-al (8.228 %) and pcymene (6.429 %). Twenty nine compounds were identified in the coriander oil – β-linalool (58.141 %), α-pinene (8.731 %), γ-terpinene (6.347 %) and p-cymene (5.227 %). Twenty nine compounds were identified in the cardamom oil – α-terpinyl acetate (39.032 %), eucalyptol (31.534 %), β-linalool (4.829 %), sabinene (4.308 %) and α-terpineol (4.127 %). The antimicrobial activity of essential oils against pathogenic (Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Salmonella sp. (clinical isolate), Staphylococcus aureus ATCC 6538P, Proteus vulgaris G) microorganisms by disc-diffusion method was examined. Gram-positive bacteria were more sensitive to the oils (inhibition zones being between 8 and 12.5 mm) and the minimum inhibitory concentration was more than 600 ppm; Gram-negative bacteria were less sensitive. The obtained essential oils are suitable for use as biopreservative agents.

scholarly journals Antibacterial Activity of Piperine and Black Pepper Oil

2018 ◽  
Vol 15 (4) ◽  
pp. 877-880 ◽  
Author(s):  
Dalia M. Hikal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Black Pepper ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Inhibition Activity ◽  
Gram Positive Bacteria ◽  
Spore Forming Bacteria ◽  
Agar Well Diffusion Method

Black pepper (Piper nigrum L.) is known as king of spices and it's sharp taste is due to the presence of piperine which is the main bioactive alkaloid in the fruit. In the present study both of piperine and black pepper oil in different concentrations evaluated for their antimicrobial activity against Staphylococcus aureus (G+ coccoid shaped bacteria), Bacillus subtilis (G+ long spore forming bacteria), Salmonella sp and E.coli (G- short rod bacteria). The inhibition activity was measured by using agar well diffusion method. Piperine and black pepper oil showed antibacterial activity with all tested Gram positive bacteria with zones ranged from 8.23-18.1mm and 3.14-10.43,respectively. The results showed that piperine is an excellent antibacterial agent with all tested bacteria.

Essential Oil Composition and Antimicrobial Activity of Diplotaenia damavandica

2005 ◽  
Vol 60 (11-12) ◽  
pp. 821-825 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Dina Azizian ◽  
Ali Sonboli ◽  
Peyman Salehi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Endemic Plant ◽  
Main Components ◽  
Disk Susceptibility

Antimicrobial activity of the essential oils obtained from leaves, root and the seeds of Diplotaenia damavandica Mozaffarian, Hedge & Lamond, an endemic plant to Iran, was determined against 10 microorganisms using the disk susceptibility test as well as measuring minimum inhibitory concentrations. The results showed that all three oils had antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. The essential oil from the leaves had the highest antimicrobial activity against all test microorganisms including the fungal strains. The essential oils compositions were analyzed and determined by GC and GC-MS. The oils analyses resulted in the identification of 16, 17 and 20 compounds representing 94.2%, 96.4% and 95.1% of the total oils, respectively. The main components of the leaf essential oils were (Z)-β-ocimene (21.6%), α-phellandrene (21.3%) and terpinolene (20%). Dill apiol (30.1%) and γ-terpinene (16.2%) were the main components of the root and seed essential oils, respectively.

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

scholarly journals Chemical composition and antimicrobial activity of extracts from thyme and rosemary against Staphylococcus aureus and Candida albicans

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9656-9671
Author(s):  
Akram Sabzikar ◽  
Seyyed Khalil Hosseinihashemi ◽  
Younes Shirmohammadli ◽  
Abbas Jalaligoldeh
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Rosemary Extract ◽  
Gram Positive Bacteria ◽  
Main Components

Different concentrations of ethanolic extracts of thyme (Zataria multiflora) and rosemary (Rosmarinus officinalis) were evaluated to determine their antimicrobial activity using the agar-well diffusion method. The values of inhibition zone diameter (IZD) for Candida albicans fungus and Staphylococcus aureus Gram-positive bacteria were determined. The bioactivities of two various extracts were studied, and the chemical composition of the extracts were identified using gas chromatography-mass spectrometry (GC-MS) technique. The results of the test showed that at concentrations of 10% and 40% thyme extract, the values of IZD were 12.5 mm and 23.3 mm, respectively, against the growth of S. aureus, which were higher than C. albicans (7.0 mm and 22.5 mm, respectively). The rosemary extract at concentrations of 20% and 60% showed lower antibacterial activity against S. aureus (4.7 mm and 8.7 mm IZD, respectively) and lower antifungal activity against C. albicans (12.2 mm and 1.7 mm IZD, respectively). At a concentration of 40% thyme extract, the highest antibacterial (23.3 mm IZD) and antifungal (22.5 mm IZD) activities were observed. The GC/MS analysis showed that carvacrol (52.3%), linalool L (16%), and thymol (9.6%) were the main components of thyme extract, while in the rosemary extract β-amyrone (18.0%), verbenone (8.0%), and 1,8-cineole (7.26%) were the major constituents.

scholarly journals CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS OF FOUR VARIETIES OF LIPPIA MULTIFLORA IN BENIN

2019 ◽  
Author(s):  
GANDONOU Dossa Clément ◽  
BAMBOLA Bouraïma ◽  
TOUKOUROU Habib ◽  
GBAGUIDI Ahokannou Fernand ◽  
DANSOU Christian ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Lippia Multiflora ◽  
Natural Substances

Present study involves the study of the chemical composition of the essential oils extracted from the leaves by gas chromatography and gas chromatography coupled with mass spectrometry of Lippia multiflora harvested in the regions of Kétou, Savalou, Bohicon and Mono and tested by the well diffusion method against pathogenic microorganisms. The essential oils studied are terpene compounds, aromatic compounds, aliphatic compounds and other natural substances. The inhibition zone diameters determined allowed us to evaluate their degree of germ sensitivity of the strains tested. Essential oils extracted from Lippia multiflora harvested in these areas have the most pronounced antimicrobial activity. In total, the essential oils tested have different and specifically a degree of sensitivity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis A24974, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa except that harvested in the Savalou who does not have no degree of sensitivity on Pseudomonas aeruginosa. This work paves the way for food preservation with extracted natural substances and also the formulation of natural antimicrobials for this fact.

scholarly journals Composition and Antimicrobial Activity of the Essential Oils from Flowers of Senecio othonnae, S. racemosus, and S. nemorensis

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Osman Üçüncü ◽  
Nuran Kahriman ◽  
Salih Terzioğrlu ◽  
Şengül Alpay Karaoğrlu ◽  
Nurettin Yaylı
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Candida Tropicalis ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Sesquiterpene Hydrocarbons ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils of Senecio othonnae, S. racemosus, and S. nemorensis obtained by hydrodistillation of fresh flowers were analysed by GC-FID and GC-MS. A total of seventy-six compounds were identified, constituting over 83.1%, 97.7% and 86.8% of the total oil composition of the flowers of S. othonnae, S. racemosus, and S. nemorensis. The main components of the oils were caryophyllene oxide (18.6%), ( E)-β-farnesene (21.6%), and γ-curcumene (42.8%), respectively. Sesquiterpene hydrocarbons were the main group of constituents of all three species, forming 34.6%, 73.2%, and 81.8% of the oils, respectively. The oils of S. othonnae, and S. nemorensis showed antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, and Candida tropicalis, but that of S. racemosus was only active against Candida tropicalis.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

Antimicrobial Activity of Some Egyptian Spice Essential Oils

1989 ◽  
Vol 52 (9) ◽  
pp. 665-667 ◽  
Author(s):  
R. S. FARAG ◽  
Z. Y. DAW ◽  
F. M. HEWEDI ◽  
G. S. A. EL-BAROTY
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Microbial Growth ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Gram Positive ◽  
Filter Paper Disc ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Chemical Structures

Six spice essential oils (sage, rosemary, caraway, cumin, clove, and thyme) and their basic ingredients were tested for their inhibitory effect against 3 strains of Gram-negative bacteria, 4 strains of Gram-positive bacteria, one acid fast bacterium, and one yeast. Preliminary screening of antimicrobial activity of the essential oils was done using the filter paper disc agar diffusion method. The minimum inhibitory concentration for each essential oil against various micro-organisms was also measured. Very low concentrations (0.25 – 12 mg/ml) of the various essential oils were sufficient to prevent microbial growth. The data show that Gram-positive bacteria were more sensitive to the antimicrobial compounds in spices than Gram-negative. The inhibition zones of different microbial growth produced by various essential oils were similar to those produced by their basic compounds. Thyme and cumin oils possessed very strong antimicrobial activity compared with the other essential oils. There was a relationship between the chemical structures of the most abundant compounds in the essential oils under investigation and the antimicrobial activity.

scholarly journals Antimicrobial Activity of Plant Extracts againstBacillus Subtilis,Staphylococcus Aureusand Escherichia Coli

2011 ◽  
Vol 8 (s1) ◽  
pp. S282-S284 ◽  
Author(s):  
Liliwirianis N ◽  
Wan Zuraida Wan Mohd Zain ◽  
Jamaluddin Kassim ◽  
Shaikh Abdul Karim
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Stemona Tuberosa ◽  
Elephantopus Scaber

Local herbs have many potential that may be active with antimicrobial activity. A screening was conducted with 11 species of herbs collected in UiTM Pahang Forest Reserve.Epipremnumsp.,Zingibersp.Tetracera indica, Tectaria crenata, Piper stylosum, Homalomena propinque, Goniothalamus sp., Elephantopus scaber, Mapania patiolale, Melastomasp.,Stemona tuberosa, Phullagathis rotundifolia, Thotea grandifoliaandSmilaxsp. were extracted with methanol to obtain their crude. The agar diffusion method using blank disc of 6 mm diameter were loaded with 1000 µg/mL of methanol crude and applied to the inoculate plate was used to assess the antimicrobial activity against two gram positive bacteria (Bacillus subtilisandStaphylococcus aeureus) and one gram negative bacteria (Escherichia coli). The results evaluated as the diameter of the inhibition zone of microbial growth, showed that all the extracts were active against gram-positive bacteria and gram-negative bacteria. The extract ofStemona tuberosewas found to be the most active against theE. coliandS. aeureuswhilePiper stylosumactive againstB. subtilis.

scholarly journals Antibacterial Activity and Reversal of Multidrug Resistance of Tumor Cells by Essential Oils from Fresh Leaves, Flowers, and Stems of Montanoa quadrangularis Schultz Bipontinus (Asteraceae) Collected in Mérida—Venezuela

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 605
Author(s):  
Janne Rojas ◽  
Gautier Mark-Arthur Ndong Ntoutoume ◽  
Patrick Martin ◽  
Marielba Morillo
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Diffusion Method ◽  
Tween 20 ◽  
Bacterial Strains ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Human Mdr1 ◽  
Main Components ◽  
Mouse Lymphoma

Essential oils obtained by hydrodistillation of Montanoa quadrangularis leaves, flowers, and stems were analyzed by GC and GC/MS techniques revealing myrcene, limonene, β-phellandrene, and sabinene among the main components. The aim of the present study was to evaluate the MDR modulator activity on human MDR1 gene transfected mouse lymphoma cell line and the antimicrobial activity on the essential oils obtained from different parts of the species under investigation. The results revealed that MQL caused a similar increase in the fluorescence activity of the cells at 0.02 μL/mL comparing to the Verapamil® value. The antimicrobial assay was carried out according to the disc diffusion method. Five different bacterial strains (Staphylococcus epidermidis, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli AG 100, and Escherichia coli AG100A) were treated with the essential oils and the zones of inhibition were determined on TSA plates and TSA agar plates supplemented with Tween 20. MQF and MQL showed activity against B. subtilis, S. epidermidis, and E. coli AG 100A while MQS was only active against E. coli AG 100A on TSA agar plates experiment. In case of TSA agar plates supplemented with 0.1 v/v% Tween 20 detergent, MQF showed inhibition on B. subtilis, S. epidermidis, and E. coli AG 100A; MQL was active against B. subtilis, E. coli AG 100, and E. coli AG 100A while MQS was only active against E. coli AG 100A.

Sign in / Sign up

Export Citation Format

Share Document