scholarly journals Application of central composite design to optimize the amount of carbon source and prebiotics for Bifidobacterium bifidum BB01

2016 ◽  
Vol 20 (1) ◽  
pp. 41-52
Author(s):  
Guowei Shu ◽  
Ni Lei ◽  
He Chen ◽  
Man Hu ◽  
Hui Yang
Keyword(s):  
Carbon Source ◽  
Central Composite Design ◽  
Culture Medium ◽  
Ph Value ◽  
Bifidobacterium Bifidum ◽  
Optimum Proportion ◽  
Central Composite

Abstract The objective of the present study was to obtain the optimum proportion of the carbon source and prebiotics for Bifidobacterium bifidum BB01 by the central composite design (CCD). The effect of carbon source (lactose) and two prebiotics (inulin and fructooligosaccharides) on the BB01 were observed by measuring the OD600 value, pH value and the viable counts at 18h. The final optimized concentrations of carbon source and prebiotics were: lactose 1.6%, inulin 0.26%, and fructooligosaccharides 0.22%. The result indicates that the growth of B. bifidum BB01 shows an significant increase in the optimized culture medium (p < 0.05), the OD600 value reached 1.434 at 18h, which increased 6.58% compared to the control. And the viable counts of B. bifidum BB01 increased 24.36% and reached (2.17±0.06) ×109cfu/mL. The results show that the optimization of the carbon source and prebiotics using CCD in this study is workable and necessary.

scholarly journals Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

2016 ◽  
Vol 20 (1) ◽  
pp. 53-64 ◽  
Author(s):  
He Chen ◽  
Jianbo Kou ◽  
Man Hu ◽  
Guowei Shu
Keyword(s):  
Response Surface Methodology ◽  
Carbon Source ◽  
Central Composite Design ◽  
Liquid Medium ◽  
Nitrogen Source ◽  
Bifidobacterium Bifidum ◽  
Optimal Conditions ◽  
Medium Carbon ◽  
Central Composite ◽  
Mrs Medium

Abstract In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.

scholarly journals Aplikasi Koagulan Biji Asam Jawa dalam Penurunan Konsentrasi Zat Warna Drimaren Red pada Limbah Tekstil Sintetik pada Berbagai Variasi Operasi

2018 ◽  
Vol 12 (2) ◽  
pp. 40
Author(s):  
Angela Martina ◽  
Dian Santoso Effendy ◽  
Jenny Novianty M Soetedjo
Keyword(s):  
Waste Water ◽  
Central Composite Design ◽  
Textile Industry ◽  
Ph Value ◽  
Production Capacity ◽  
Natural Coagulant ◽  
Design Expert ◽  
Tamarind Seed ◽  
Coagulation And Flocculation ◽  
Central Composite

A B S T R A C TSince textile industries use a lot of water in their processes, a huge volume of waste water containing dyes are produced by the increase of the production capacity. Coagulation and flocculation are the common processes applied since they can effectively decrease the dye concentration in the waste water. These treatments usually utilize chemical coagulant and flocculant which are expensive and non-biodegradable. In this research, tamarind seed as one of biobased-coagulants was studied and developed to reduce drimaren dark red HF-CD concentration which is used widely in textile industry in the synthetic waste water. The research was designed using Design Expert 7.0.0, Central Composite Design with range of variables as follows: pH (2-7), tamarind seed concentration (1-3 g/L), and dye concentration (20-30 ppm). The result shows a promising application of natural coagulant up to 94.25% decrease of dye concentration in the optimum condition of 3.68 g/L tamarind seed concentration, 25 ppm dye concentration and pH value of 4.5.Keywords: coagulation; dye concentration acid; natural coagulant; tamarind seedsA B S T R A KIndustri tekstil merupakan industri yang banyak menggunakan air dalam proses produksinya sehingga menghasilkan limbah yang mengandung zat warna tekstil dengan volume yang besar. Pengolahan yang umum digunakan untuk mengolah limbah tekstil ini adalah koagulasi dan flokulasi. Metode ini efektif dalam mengurangi konsentrasi zat warna pada air limbah. Koagulan yang digunakan pada penelitian ini adalah koagulan alami yang terbuat dari biji asam jawa. Penelitian ini bertujuan untuk mengetahui kemampuan dan kondisi optimum biji asam jawa sebagai koagulan alami dalam menurunkan konsentrasi zat warna pada limbah tekstil. Limbah tekstil yang digunakan merupakan limbah sintetik zat warna drimaren dark red HF-CD. Rancangan penelitian dibuat menggunakan Design Expert 7.0.0 metode central composite design dengan memvariasikan variabel pH (2-7), dosis koagulan (1-3 g/L), dan dosis zat warna (20-30 ppm). Kondisi terbaik yang didapat dari penelitian diperoleh pada pH 4,5, dosis koagulan 3,68 g/L, dosis zat warna 25 ppm dengan hasil persen penurunan konsentrasi zat warna sebesar 94,29%.Kata kunci: biji asam jawa; koagulasi; koagulan alami; konsentrasi zat warna

scholarly journals Removal of Mefenamic Acid from Aqueous Solution by Fenton Process: Optimization Using Response Surface Methodology with Central Composite Design

2021 ◽  
Vol 20 (5) ◽  
Author(s):  
R. Deepa ◽  
G. Madhu ◽  
Roy M Thomas ◽  
V. Sivanandan Achari
Keyword(s):  
Aqueous Solution ◽  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Process Optimization ◽  
Mefenamic Acid ◽  
Ph Value ◽  
Main Process ◽  
Fenton Process ◽  
Central Composite

In the present study, the three main process parameters in the Fenton process for the removal of pharmaceutical compound Mefenamic acid from an aqueous solution were optimized using response surface methodology (RSM). Central composite design (CCD) was used for process optimization. The primary and secondary interaction effects of the selected parameters such as H2O2, Fe2+ and pH on the removal of mefenamic acid were examined. A mathematical model for the removal process based on the selected variables was developed. The interaction effect between the chosen parameters shows that the removal of mefenamic acid was enhanced in the acidic pH range at a high concentration of H2O2 and in a medium concentration level of the catalyst Fe2+. The removal efficiency of 81.24% was obtained for mefenamic acid at the optimized condition of variables such as 9.36 mM H2O2, 0.058 mM Fe2+and at a pH value of 2.1.

Development and optimization of solid lipid nanoparticles of amikacin by central composite design

2009 ◽  
Vol 00 (00) ◽  
pp. 090721051030036-8
Author(s):  
Jaleh Varshosaz ◽  
Solmaz Ghaffari ◽  
Mohammad Reza Khoshayand ◽  
Fatemeh Atyabi ◽  
Shirzad Azarmi ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Central Composite

Formulation Design and Optimization of Repaglinide Solid Dispersions by Central Composite Design

2018 ◽  
Vol 11 (6) ◽  
pp. 4337-4348
Author(s):  
Bhikshapathi D. V. R. N. ◽  
Srinivas I
Keyword(s):  
Central Composite Design ◽  
Dissolution Rate ◽  
Solid Dispersion ◽  
Solid Dispersions ◽  
Solvent Evaporation ◽  
Release Mechanism ◽  
Solvent Evaporation Method ◽  
Onset Of Action ◽  
Evaporation Method ◽  
Central Composite

Repaglinide is a pharmaceutical drug used for the treatment of type II diabetes mellitus, it is characterized with poor solubility which limits its absorption and dissolution rate and delays onset of action. In the present study, immediate release solid dispersion of repaglinide was formulated by solvent evaporation technique. Repaglinide solid dispersions were prepared using PEG 8000, Pluronic F 127 and Gelucire 44/14 by solvent evaporation method. A 3-factor, 3-level central composite design employed to study the effect of each independent variable on dependent variables. FTIR studies revealed that no drug excipient interaction takes place. From powder X-ray diffraction (p-XRD) and by scanning electron microscopy (SEM) studies it was evident that polymorphic form of repaglinide has been converted into an amorphous form from crystalline within the solid dispersion formulation. The correlation coefficient showed that the release profile followed Higuchi model anomalous behavior and hence release mechanism was indicative of diffusion. The obtained results suggested that developed solid dispersion by solvent evaporation method might be an efficacious approach for enhancing the solubility and dissolution rate of repaglinide.

Formulation and Optimization of 5-Fluorouracil Loaded Chitosan Nanoparticles Employing Central Composite Design

2012 ◽  
Vol 2 (4) ◽  
pp. 281-289
Author(s):  
Rattan Lal ◽  
Rakesh Kumar Marwaha ◽  
Deepti Pandita ◽  
Harish Dureja
Keyword(s):  
Central Composite Design ◽  
Chitosan Nanoparticles ◽  
Central Composite
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