scholarly journals A Comparison of Different Methods for Fissure Caries Detection

2016 ◽  
Vol 43 (1) ◽  
pp. 30-38 ◽  
Author(s):  
K. Peycheva ◽  
E. Boteva
Keyword(s):  
In Vitro Study ◽  
The Body ◽  
Diagnostic Methods ◽  
Laser Fluorescence ◽  
Caries Detection ◽  
Caries Lesion ◽  
Occlusal Surfaces ◽  
Icdas Ii ◽  
Permanent Molars

Summary The aim of the study is to compare the diagnostic capabilities of three different diagnostic methods: Quantitative Laser Fluorescence (QLF) − DIAGNOdent Classic (DD), Light-Induced Fluorescence (LIF) − SoproLife daylight and blue fluorescence, and their relevance to ICDAS II system in detection of fissure caries lesions in permanent molars. Permanent molars (n = 45) are divided in two groups: 1) third molars, n = 35; 2) first and second molars, n = 10. They are examined by 2 examiners with and without magnification x5 using ICDAS II, SoproLife “day light” and “blue light” (405nm), LIF, DIAGNOdent Classic − emitting laser light on 655nm, QLF. The results are proven with histological bucco-lingual or mesio-distal sections through the body of the lesion with diamond blade rinsed with water. Photos of all occlusal surfaces of the molars are taken before and after the sections. The lowest overdiagnosis rate is found with SoproLife camera. When visual examination is applied overdiagnoses are fewer than with DD. DD is not capable to differentiate white and brown spots from a caries lesion. Soprolife is not capable to differentiate brown spots from a caries lesion. The most accurate method in this in vitro study for diagnosis of fissure caries is LIF (SoproLife) − 75.6% of the teeth are correctly diagnosed, followed by ICDAS (57.8%) and QLF (DIAGNOdent) (40%).

scholarly journals The Performance of ICDAS-II Using Low-Powered Magnification with Light-Emitting Diode Headlight and Alternating Current Impedance Spectroscopy Device for Detection of Occlusal Caries on Primary Molars

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Timucin Ari ◽  
Nilgun Ari
Keyword(s):  
Impedance Spectroscopy ◽  
Light Emitting Diode ◽  
Clinical Diagnostics ◽  
Caries Detection ◽  
Primary Molars ◽  
Occlusal Caries ◽  
Light Emitting ◽  
Occlusal Surfaces ◽  
Icdas Ii

Early detection of occlusal caries in children is challenging for the dentists, because of the morphology of pit and fissures. The aim of this study was to compare in vitro the diagnostic performance of low-powered magnification with light-emitting diode headlight (LPMLED) using ICDAS-II criteria and AC Impedance Spectroscopy (ACIS) device, on occlusal surfaces of primary molars. The occlusal surfaces of 18 extracted primary molars were examined blindly by two examiners. The teeth were sectioned and examined under light microscopy using Downer’s histological criteria as gold standard. Good to excellent inter- and intraexaminer reproducibility, higher sensitivity, specificity, and AUC values were achieved by LPMLED at D1 threshold. Also the relationship between histology and LPMLED was statistically significant. In conclusion visual aids have the potential to improve the performance of early caries detection and clinical diagnostics in children. Despite its potential, ACIS device should be considered as an adjunct method in detecting caries on primary teeth.

Proximal caries lesion detection using the Canary Caries Detection System: an in vitro study

2015 ◽  
Vol 7 (4) ◽  
pp. 383-390 ◽  
Author(s):  
Janja Jan ◽  
Wan Zaripah Wan Bakar ◽  
Sapna M. Mathews ◽  
Linda O. Okoye ◽  
Benjamin R. Ehler ◽  
...  
Keyword(s):  
Detection System ◽  
In Vitro Study ◽  
Lesion Detection ◽  
Vitro Study ◽  
Caries Detection ◽  
Proximal Caries ◽  
Caries Lesion

Diagnostic Performance of Different Methods in Detecting Incipient Non-Cavitated Occlusal Caries Lesions in Permanent Teeth

2019 ◽  
Vol 43 (3) ◽  
pp. 173-179
Author(s):  
Murat Ünal ◽  
Arzu Koçkanat ◽  
Seniha Güler ◽  
Esra Gültürk
Keyword(s):  
Intraclass Correlation ◽  
In Vitro Study ◽  
Roc Curves ◽  
Caries Detection ◽  
Permanent Teeth ◽  
Kappa Statistics ◽  
Occlusal Caries ◽  
Diagnodent Pen ◽  
Icdas Ii

Aim: This in vitro study was aimed to investigate the performance of the new caries detection tools on the incipient occlusal caries. Study design: In our study, 100 permanent molar teeth, which were considered to have incipient, enamel caries (D1 and D2 threshold values) according to ICDAS II score. After the visual examination, caries measurements have been applied to all teeth by using DIAGNOdent pen (DP), CarieScan PRO (CP) and SoproLife camera (SC). In addition, in vitro examinations were repeated 2 weeks later. After sectioning and evaluation in stereomicroscope, the lesion depth was determined with histological criteria that are accepted for the gold standard for this research. Sensitivity, specificity, accuracy and area under the ROC curve were calculated at D1 and D2 thresholds. The intra-examiners' reproducibility were analysed using Cohen's kappa statistics and intraclass correlation coefficient (ICC). Results: Intra-examiner repeatability values varied from 0.94 to 0.99 and kappa values ranged from 0.90 to 0.99. While the highest sensitivity and specificity value showed for SC at D1 and D2 thresholds, CP presented the lowest values. The area under ROC curves (Az) varied from 0.70 to 0.99. ICDAS II showed the highest area under ROC curves (Az). Conclusions: ICDAS II, visual diagnostic method by itself is considered to sufficient in order to diagnose incipient occlusal caries. SC may be used as an alternative method for the ICDAS II, since its advantages by displaying caries lesion on the computer monitor, follow-up and motivation of the patients provided.

In vitro study of caries detection through sound dentin using a laser fluorescence device, DIAGNOdent

2003 ◽  
Vol 111 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Yukiteru Iwami ◽  
Ayako Shimizu ◽  
Hiroko Yamamoto ◽  
Mikako Hayashi ◽  
Fumio Takeshige ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Laser Fluorescence ◽  
Caries Detection

scholarly journals Detection of Caries Around Resin-Modified Glass Ionomer and Compomer Restorations Using Four Different Modalities In Vitro

2018 ◽  
Vol 6 (3) ◽  
pp. 47 ◽  
Author(s):  
Tamara Abrams ◽  
Stephen Abrams ◽  
Koneswaran Sivagurunathan ◽  
Veronika Moravan ◽  
Warren Hellen ◽  
...  
Keyword(s):  
Assessment System ◽  
Laser Fluorescence ◽  
Caries Detection ◽  
Signal Interference ◽  
Glass Ionomer ◽  
Icdas Ii ◽  
Resin Modified Glass Ionomer ◽  
Tooth Surfaces ◽  
Natural Decay

The aim of this study was to evaluate the ability of visual examination (International Caries Detection and Assessment System—ICDAS II), light-emitting diodes (LED) fluorescence (SPECTRA), laser fluorescence (DIAGNODent, DD), photothermal radiometry and modulated luminescence (PTR-LUM, The Canary System, CS) to detect natural decay beneath resin-modified glass ionomer (RMGIC) and compomer restorations in vitro. Twenty-seven extracted human molars and premolars, consisting of 2 control teeth, 10 visually healthy/sound and 15 teeth with natural cavitated lesions, were selected. For the carious teeth, caries was removed leaving some carious tissue on one wall of the preparation. For the sound teeth, 3 mm deep cavity preparations were made. All cavities were restored with RMGIC or compomer restorative materials. Sixty-eight sites (4 sites on sound unrestored teeth, 21 sound sites and 43 carious sites with restorations) were selected. CS and DD triplicate measurements were done at 2, 1.5, 0.5, and 0 mm away from the margin of the restoration (MOR). SPECTRA images were taken, and two dentists provided ICDAS II scoring for the restored surfaces. The SPECTRA data and images were inconclusive due to signal interference from the restorations. Visual examinations of the restored tooth surfaces were able to identify 5 of the 15 teeth with caries. In these situations, the teeth were ranked as having ICDAS II 1 or 2 rankings, but they could not identify the location of the caries or depth of the lesion. CS and DD were able to differentiate between sound and carious tissue at the MOR, but larger variation in measurement, and poorer accuracy, was observed for DD. It was concluded that the CS has the potential to detect secondary caries around RMGIC and compomer restorations more accurately than the other modalities used in this study.

In vitro and in vivo Evaluation of the Radiochemical Compound Based on 99mTechnetium Labelled DARPin 9_29 for Molecular Visualization of Malignancies Overexpressing Her2/neu

2020 ◽  
Vol 65 (1) ◽  
pp. 37-41
Author(s):  
O. Bragina ◽  
A. Vorobyeva ◽  
V. Tolmachev ◽  
A. Orlova ◽  
V. Chernov ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Radiochemical Yield ◽  
Blood Stream ◽  
The Body ◽  
Diagnostic Methods ◽  
In Vivo Studies ◽  
In Vivo Evaluation ◽  
High Accumulation

Purpose: Evaluation of a radiopharmaceutical based on 99mTc-labeled targeted molecules DARPin9_29 for radionuclide diagnostics of malignancies with Her2/neu overexpression. Material and methods: The DARPin9_29 sequence was amplified from the plasmid pET-DARP-6HIS for the DARPin9_29-His6 gene expression in E. coli cells. The eluent of 99mTcO4– (400–500 μl, 4 GBq) was added to the kit and incubated at a temperature of 100 °C for 20 minutes. After incubation, 40 μl of tricarbonyl technetium was added to 168 μg of DARPin9_29 in 100 μl of PBS (sodium phosphate buffer), followed by incubation at 40 °C for 60 minutes. The radiochemical yield and purity were determined by thin layer radiochromatography, the purification was performed using NAP-5 cleansing columns (GE Healthcare). Cell lines with different levels of Her2/neu expression were used: SKOV-3> BT474 >> DU-145 for the determination of the radiopharmaceutical specificity. Her2/neu expressing cell line SKOV-3 was used for in vitro study. The study was conducted 6 hours after the administration of the drug. Results: The radiochemical yield was 72 ± 8 %, the radiochemical purity after purification was 98.7 ± 1.0 %. The stability in PBS (phosphate buffered saline) solution after 1 hour was 99.8 ± 0.2; after 3 hours – 98.2 ± 0.1. In vitro studies showed that the accumulation of explored compound was directly proportional to the level of Her2/neu expression in cells, while blocking the receptors with an excess of unlabeled protein showed a significant reduction in binding in the group of cells. Data on biodistribution and SPECT/CT in the body of the animal BALB/c nu/nu demonstrated rapid removal of the compound from the blood stream and high accumulation in the liver, kidney and bladder 6 hours after the introduction of the radiopharmaceutical. Conclusion: The studies demonstrated high radiochemical yields and purity, as well as stability of the studied compound. The results of in vitro and in vivo analysis showed the specificity and affinity of the radiopharmaceutical to the Her2/neu receptor on the surface of tumor cells. The high accumulation of the drug in the liver and kidneys, detected in in vivo studies, is probably due to the lipophilicity of the 99mTc(CO)3-histidine tag and indicates the limitation of its further clinical use in assessing the condition of the above organs, which will require additional diagnostic methods, as well as possible modification chemical structure.

scholarly journals Diagnostic Drama. Use of ICDAS II and Fluorescence-Based Intraoral Camera in Early Occlusal Caries Detection: A Clinical Study

2020 ◽  
Vol 17 (8) ◽  
pp. 2937
Author(s):  
Marta Mazur ◽  
Maciej Jedliński ◽  
Artnora Ndokaj ◽  
Denise Corridore ◽  
Antonello Maruotti ◽  
...  
Keyword(s):  
Subjective Evaluation ◽  
Assessment System ◽  
Diagnostic Methods ◽  
Caries Detection ◽  
Kappa Statistics ◽  
Occlusal Caries ◽  
Posterior Teeth ◽  
Invasive Approach ◽  
Occlusal Surfaces ◽  
Icdas Ii

Background: Early diagnosis of occlusal caries is of paramount importance for a minimally invasive approach in dentistry. The aim of the present in vivo clinical prospective study was to compare the diagnostic outcomes of visual subjective evaluation between the International Caries Detection and Assessment System (ICDAS-II) and an intraoral fluorescence-based camera (VistaCam iX Proof, Dürr Dental, Bietigheim-Bissingen, Germany) for the detection of pits and fissures in early caries lesions of posterior teeth. Methods: The study included 1011 posterior teeth in 255 patients aged 13–20 years (mean age 16 ± 2.2 years). Two blinded operators evaluated all the occlusal surfaces and the first assigned an ICDAS-II code, while the second assessed the VistaCam score: sound enamel (score 0–1.2); initial enamel decay (score 1.2–1.5); dentine caries (score 1.5–3). Results: Some 283 (28%) of the assessed teeth were ICDAS-II code 0; 334 (33%) code 1; 189 (18.7%) code 2; 176 (17.4%) code 3; and 29 (2.9%) code 4. The level of agreement between the two procedures was expressed by using Cohen’s and Fleiss’ kappa statistics and performing McNemar’s test. VistaCam assessed in 513 (50.7%) sound enamel; in 292 (28.9%) initial enamel decay; and in 206 (20.4%) dentine caries. Conclusions: This comparative study showed a poor agreement between the two diagnostic methods, especially between ICDAS-II 0, 1 and 2 codes and fluorescence assessments.

scholarly journals Occlusal caries diagnosis in permanent teeth: an in vitro study

2005 ◽  
Vol 19 (4) ◽  
pp. 243-248 ◽  
Author(s):  
Gisele Angnes ◽  
Vivian Angnes ◽  
Rosa Helena Miranda Grande ◽  
Márcio Battistella ◽  
Alessandro Dourado Loguercio ◽  
...  
Keyword(s):  
Visual System ◽  
Roc Curve ◽  
In Vitro Study ◽  
Laser Fluorescence ◽  
Permanent Teeth ◽  
Occlusal Caries ◽  
Caries Diagnosis ◽  
Occlusal Surfaces ◽  
Sensitivity Specificity

The reduction in caries prevalence has not occurred uniformly for all dental surfaces. As the occlusal surfaces are still the most likely sites for the development of lesions, new methods of diagnosis are still being evaluated. This study compared a laser fluorescence (LF) system (DIAGNOdent) with the Ekstrand’s visual system for in vitro detection of occlusal caries. A total of 57 extracted molars with macroscopically intact occlusal surfaces were selected. Two-examiners assessed 110 sites by visual inspection (VI) and LF. After ten days from the first measurement, all teeth were re-evaluated through the same methods by each examiner. Caries extension was histologically assessed (X 40). The methods were compared by means of sensitivity, specificity, intra- and inter-examiner reproducibility and area under the ROC curve. The kappa’s test showed good intra- and inter-examiner reproducibility for both methods. VI and LF showed similar sensitivities for both examiners, however, VI showed higher specificities than LF. The overall analysis, as demonstrated by the area under the ROC curve, showed that VI had a better performance than the LF device. It was concluded that the Ekstrand’s visual system is more reliable than the LF device. LF should be considered only as an adjuvant for occlusal caries diagnosis.

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