scholarly journals Inference of hierarchical regulatory network of TCF7L2 binding sites in MCF7 cell line

2016 ◽  
Vol 9 (1/2) ◽  
pp. 25 ◽  
Author(s):  
Yao Wang ◽  
Rui Wang ◽  
Victor X. Jin
Keyword(s):  
Cell Line ◽  
Binding Sites ◽  
Regulatory Network ◽  
Mcf7 Cell ◽  
Mcf7 Cell Line

scholarly journals Methoxy and bromo scans on N-(5-methoxyphenyl) methoxybenzenesulphonamides reveal potent cytotoxic compounds, especially against the human breast adenocarcinoma MCF7 cell line

2021 ◽  
Vol 36 (1) ◽  
pp. 1029-1047
Author(s):  
Myriam González ◽  
María Ovejero-Sánchez ◽  
Alba Vicente-Blázquez ◽  
Manuel Medarde ◽  
Rogelio González-Sarmiento ◽  
...  
Keyword(s):  
Cell Line ◽  
Mcf7 Cell ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cytotoxic Compounds ◽  
Mcf7 Cell Line

scholarly journals Novel 1,8-Naphthyridine Derivatives: Design, Synthesis and in vitro screening of their cytotoxic activity against MCF7 cell line

2019 ◽  
Vol 17 (1) ◽  
pp. 943-954
Author(s):  
Abeer N. Al-romaizan ◽  
Thoraya S. Jaber ◽  
Nesreen S. Ahmed
Keyword(s):  
Cell Line ◽  
Human Breast Cancer ◽  
Mcf7 Cell ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
The Other ◽  
Human Breast ◽  
Design Synthesis ◽  
Mcf7 Cell Line

AbstractA series of new 2-phenyl-7-methyl-1,8-naphthyridine derivatives with variable substituents at C3 were synthesized for an in vitro evaluation of their anticancer activity against human breast cancer cell line (MCF7). On one hand, compounds 3f, 6f, 8c, and 10b showed IC50 values (6.53, 7.88, 7.89, 7.79 μM, respectively) compared to that of the mentioned drug staurosparine (IC50 = 4.51 μM). On the other hand, derivatives 10c, 8d, 4d, 10f and 8b displayed better activity than staurosporin with IC50 values (1.47, 1.62, 1.68, 2.30, 3.19 μM, respectively).

scholarly journals Evaluation of anti-proliferative activity of simvastatin and atorvastatin on MCF7 cell line compared with doxorubicin using MTT test

2018 ◽  
pp. 163-169
Keyword(s):  
Cell Line ◽  
Proliferative Activity ◽  
Mcf7 Cell ◽  
Mevalonate Pathway ◽  
Pleiotropic Effect ◽  
Receptor Protein ◽  
Dependent Manner ◽  
Proliferative Effect ◽  
Mcf7 Cell Line ◽  
Dose Dependent

Statins are effective to lower the cholesterol level and protect against cardiovascular disease. Recent studies showed that statins have pleiotropic effect and can be used to treat many types of diseases like neurodegenerative disorders, stroke, and cancer. Statins inhibit cell proliferation by suppression of mevalonate pathway leading to desregu-lation of cell signal transduction of some membrane receptor protein which is important for gene transcription. This study was done to evaluate the anti-proliferative activity of simvastatin and atorvastatin on MCF7 cell line alone and in combination with anathracycline chemotherapy drug (doxo-rubicin) using MTT assay. The results showed that simvastatin and atorvastatin had significant anti-proliferative effect on MCF7 cell line in dose-dependent manner with an IC50 10.10 μM and 12.3 μM respect-tively. Moreover, the combination of atorvastatin and simvastatin with (1 μM) doxorubicin had higher cytotoxic effect with an IC50 = 0.07 μM and 0.05μM respectively than doxoru-bicin alone IC50 = 1.9 μM. In conclusion, simvastatin and atorvastatin had anti-proliferative effect on MCF7 cell line and displayed significant synergism with doxorubicin which will help in enhancing efficacy of doxorubicin and decrease the adverse effect.

scholarly journals Synthesis and antitumor activity of new isolated and fused heterobicyclic nitrogen systems containing 1,3,4-thiadiazole moiety derived from N1,N2-diaryl hydrazine compound

2020 ◽  
Vol 9 (1) ◽  
pp. 935-940
Keyword(s):  
Antitumor Activity ◽  
Cell Line ◽  
Spectral Data ◽  
13C Nmr ◽  
Mass Spectra ◽  
Mcf7 Cell ◽  
Nitrogen Compounds ◽  
Mcf7 Cell Line ◽  
Ft Ir

New pyrazolyls and/ or 1,2,4-triazines bearing 1,3,4-thiadiazole moiety (3-10) have been synthesized via an interaction between 2-[5-(2-(4-chlorophenyl)hydrazineyl)-1,3,4-thiadiazol-2-yl]phenol and bi-oxygen/ chloro nitrogen compounds in different conditions. Former structures of the new systems have been established from their elemental and spectral data (FT-IR, 1H/ 13C NMR, Mass spectra). All the obtained compounds were evaluated against MCF7 cell line cytotoxicity and antitumor cells. Compounds 9>10>2>7>8>4>5>6 exhibited important action.

Synthesis of artemisinic acid derived glycoconjugates and their anticancer studies

2020 ◽  
Vol 18 (12) ◽  
pp. 2252-2263
Author(s):  
Tharun K. Kotammagari ◽  
Sayantan Paul ◽  
Ganesh K. Barik ◽  
Manas K. Santra ◽  
Asish K. Bhattacharya
Keyword(s):  
Cell Line ◽  
Mcf7 Cell ◽  
Click Reaction ◽  
Anticancer Activities ◽  
Artemisinic Acid ◽  
Mcf7 Cell Line ◽  
Line P

Twenty-four artemisinic acid glycoconjugate hybrids were synthesized using click reaction and evaluated for their anticancer activities against the MCF7 cell line.

scholarly journals MicroRNA-126-5p Inhibits the Migration of Breast Cancer Cells by Directly Targeting CNOT7

2020 ◽  
Vol 19 ◽  
pp. 153303382097754
Author(s):  
Yuying Miao ◽  
Jiang Lu ◽  
Baozhen Fan ◽  
Lecan Sun
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Mcf7 Cell ◽  
Bioinformatic Analysis ◽  
Negative Control ◽  
Differentially Expressed ◽  
Luciferase Reporter ◽  
Differentially Expressed Mirnas ◽  
Mcf7 Cell Line ◽  
Breast Cancer Mcf7 Cell

Background: To assess the effect of microRNA-126-5p (miR-126-5p) on the migration of the breast cancer MCF7 cell line. Methods: GSE143564 was downloaded from the Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo ) to identify the differentially expressed miRNAs between breast cancer and adjacent tissues. Quantitative reverse transcription PCR (RT-qPCR) was used to assess miR-126-5p levels in the normal 184A1 breast cell line and the breast cancer MCF7 cell line. The MCF7 cell line was then transfected with miR-126-5p mimics or corresponding negative control (NC-mimic). The proliferation and migration abilities of the MCF7 cell line were measured by methyl thiazolyl tetrazolium (MTT), Transwell and scratch healing assays. CCR4-NOT transcription complex and subunit 7 (CNOT7) expression levels in the NC-mimic and miR-126-5p mimic groups were measured by Western blot analysis. Bioinformatic analysis and a dual-luciferase reporter assay were performed to identify the miR-126-5p target gene. Results: One hundred forty-eight differentially expressed miRNAs (downregulated = 55, upregulated = 93) were identified. MiR-126-5p expression in the MCF7 cell line was significantly downregulated relative to that of 184A1 cell line (P < 0.05). Compared with that observed in the control and NC-mimic groups, cell proliferation in the miR-126-5p mimic group was significantly decreased at 48 and 72 h posttransfection (P < 0.05). In addition, the scratch healing rate and number of membrane-piercing cells in the miR-126-5p overexpression group were lower than those detected in the control and NC groups (P < 0.05). Furthermore, miR-126-5p could reduce the luciferase activity for the wild-type CNOT7 gene 3’-untranslated region (UTR) reporter (P < 0.05) but had no effect on the mutant 3’UTR reporter (P > 0.05). Compared with that observed in the NC and control groups, the levels of CNOT7 in the miR-126-5p overexpression group decreased (P < 0.05). Conclusion: Upregulation of miR-126-5p can inhibit the migration of the breast cancer MCF7 cell line, which may involve its direct targeting of the 3’UTR of CNOT7.

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