scholarly journals A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia

2018 ◽  
Vol 43 (2) ◽  
pp. 102-110 ◽  
Author(s):  
Jeremy S. Lum ◽  
Samuel J. Millard ◽  
Xu-Feng Huang ◽  
Lezanne Ooi ◽  
Kelly A. Newell
Keyword(s):  
Nucleus Accumbens ◽  
Glutamate Receptors ◽  
Metabotropic Glutamate Receptors ◽  
Metabotropic Glutamate ◽  
Group 1

Metabotropic Glutamate Receptors Regulate N-Methyl-d-Aspartate–Mediated Synaptic Transmission in Nucleus Accumbens

1997 ◽  
Vol 78 (6) ◽  
pp. 3028-3038 ◽  
Author(s):  
Gilles Martin ◽  
Zhiguo Nie ◽  
George R. Siggins
Keyword(s):  
Nucleus Accumbens ◽  
Synaptic Transmission ◽  
Glutamate Receptors ◽  
Metabotropic Glutamate Receptors ◽  
Brain Slices ◽  
Receptor Subtypes ◽  
Metabotropic Glutamate ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Martin, Gilles, Zhiguo Nie, and George R. Siggins. Metabotropic glutamate receptors regulate N-methyl-d-aspartate–mediated synaptic transmission in nucleus accumbens. J. Neurophysiol. 78: 3028–3038, 1997. We recorded intracellularly from core nucleus accumbens (NAcc) neurons in brain slices to study the regulation by metabotropic glutamate receptors (mGluRs) of pharmacologically isolated N-methyl-d-aspartate–mediated excitatory postsynaptic currents (NMDA-EPSCs). Monosynaptic NMDA-EPSCs, evoked by local stimulation, were isolated by superfusion of the non-NMDA and γ-aminobutyric acid-A (GABAA) receptor antagonists, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 μM) and bicuculline (15 μM), respectively. Trans-1-aminocyclopentane-1,3-decarboxylic acid ( trans-ACPD; 50 μM), a nonspecific group 1 and 2 mGluR agonist, had no effect on resting membrane potential (RMP) or input resistance of NAcc neurons. However, it consistently decreased NMDA-EPSC areas (time integrals) dose dependently (1–100 μM; EC50 = 8 μM) and reversibly. The specific group 1 mGluR agonists quisqualate (1–4 μM) and (RS)-3,5-dihydroxyphenylglycine (DHPG; 100 μM) did not mimic the trans-ACPD effect on NMDA-EPSCs, nor did exposure of the slice to the group 1 mGluR antagonist l(+)-2-amino-3-phosphonopropionic acid (l-AP3, 0.4 mM) inhibit the trans-ACPD effect. The putative mGluR1 and mGluR2 antagonist (+)-α-methyl-4-carboxyphenylglycine (MCPG) at 0.5 mM failed to antagonize trans-ACPD effects but at 1 mM blocked them. Both the group 2 mGluR agonist (2S,3S,4S)-α-(carboxycyclopropyl)-glycine (l-CCG-I, 2 μM) and the group 3 mGluR specific agonist l(+)-2-amino-4-phosphonobutyric acid (l-AP4, 20 μM) attenuated NMDA-EPSC areas; the effect of l-AP4 was blocked by the group 3 antagonist (S)-2-amino-2-methyl-4-phosphonobutanoic acid (MAP4; 0.5 mM). Exogenously applied NMDA, in the presence of tetrodotoxin to prevent presynaptic effects, induced inward currents that were decreased by 20 μM l-AP4 but not by 10 μM trans-ACPD. These findings suggest that NMDA receptor-mediated neurotransmission in NAcc is under dual inhibitory regulation by group 2 and 3 metabotropic receptor subtypes: l-AP4–sensitive receptors located postsynaptically and those sensitive to trans-ACPD located presynaptically.

scholarly journals Inside Front Cover: Group 1 metabotropic glutamate receptors 1 and 5 form a protein complex in mouse hippocampus and cortex

PROTEOMICS ◽  
2016 ◽  
Vol 16 (20) ◽  
pp. NA-NA
Author(s):  
Nikhil J. Pandya ◽  
Remco V. Klaassen ◽  
Roel C. van der Schors ◽  
Johan A. Slotman ◽  
Adriaan Houtsmuller ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Protein Complex ◽  
Metabotropic Glutamate Receptors ◽  
Front Cover ◽  
Metabotropic Glutamate ◽  
Mouse Hippocampus ◽  
Group 1
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