scholarly journals Dayanıklılık Geni Cre1’in Akdeniz tahıl kist nematodu, Heterodera latipons Franklin (Tylenchida: Heteroderidae)’e karşı etkinliğinin araştırılması

2014 ◽  
Vol 20 (3) ◽  
pp. 261-268 ◽  
Author(s):  
İMREN Mustafa; KASAPOĞLU
Keyword(s):  
Heterodera Latipons

scholarly journals Bioinformatics analysis of ubiquitin expression protein gene from Heterodera latipons

2019 ◽  
Vol 26 (7) ◽  
pp. 1463-1467
Author(s):  
Luma Al-Banna ◽  
Monther T. Sadder ◽  
Hamzeh A. Lafi ◽  
Ahmed A.M. Dawabah ◽  
Saleh N. Al-Nadhari
Keyword(s):  
Protein Gene ◽  
Bioinformatics Analysis ◽  
Heterodera Latipons

Histological study of syncytia induced in cereals by the Mediterranean cereal cyst nematode Heterodera latipons

Nematology ◽  
2008 ◽  
Vol 10 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Yuji Oka ◽  
Mishael Mor ◽  
Yitzhak Spiegel
Keyword(s):  
Cell Wall ◽  
Histological Study ◽  
Parenchymal Cell ◽  
Cortical Cell ◽  
Syncytium Formation ◽  
Wheat Root ◽  
Heterodera Avenae ◽  
Vascular Cylinder ◽  
Cell Layers ◽  
Heterodera Latipons

AbstractFeeding sites of Heterodera latipons in wheat, barley and Lolium rigidum were studied and compared with those of Heterodera avenae in wheat. Juveniles of H. latipons penetrated mainly differentiated roots and chose a root cortical cell as their initial syncytium cell (ISC) in wheat and barley, whilst H. avenae chose a vascular parenchymal cell as an ISC. The cell associated with females in wheat root underwent hypertrophy, and incorporated endodermis, pericycle and parenchyma cells of the vascular cylinder by cell wall dissolution. Syncytia of H. latipons contained a high number of organelles, including endoplasmic reticulum, mitochondria, plastids and amoeboid nuclei, whilst those of H. avenae were highly vacuolated. The syncytium partly occupied both the cortex and the vascular cylinder in contrast to those of H. avenae, which occupied most of the space within the vascular cylinder near the infection site. The cortical cell associated with H. latipons males did not undergo hypertrophy, but had dense cytoplasm, and incorporation of other cells by cell wall dissolution was rare. A similar syncytium formation process was observed with barley, but a cortical cell, several cell layers away from the endodermis, was chosen for the ISC. In resistant L. rigidum, H. latipons juveniles chose an endodermal cell or its neighbouring cortical cell as an ISC. Partial dissolution of cell wall was observed in neighbouring parenchymal cells, although the nematode did not develop into adults.

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Nematology ◽  
2013 ◽  
Vol 15 (6) ◽  
pp. 709-717 ◽  
Author(s):  
Fateh Toumi ◽  
Fateh Toumi ◽  
Lieven Waeyenberge ◽  
Fateh Toumi ◽  
Lieven Waeyenberge ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Actin Gene ◽  
Specific Primers ◽  
Second Stage ◽  
Specific Pcr ◽  
Pcr Products ◽  
Heterodera Latipons ◽  
Species Specific ◽  
Very High

Several Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important. Their identification, based on morphological characteristics, is not straightforward but can be made easier using molecular techniques. In this study, we developed species-specific primers for the detection of H. latipons. The actin gene of eight Heterodera species was partially sequenced and, after purifying and sequencing the PCR products, all sequences were aligned to find unique sites. The alignment showed moderate to very high similarities between the species. However, a small fragment of the actin gene was suitable for the construction of a potentially useful species-specific primer for H. latipons. The optimised PCR was subsequently tested with several populations of 14 Heterodera species and a single population of Punctodera punctata. Heterodera latipons was represented by 16 populations originating from six different countries. The primer set (Hlat-act), designed using AlleleID 7.73, was shown to be very specific. To test its sensitivity further, the PCR was conducted on DNA extracted from five second-stage juveniles (J2) of H. latipons mixed with five or 100 J2 belonging to H. avenae. The PCR was able to detect up to 1:10 dilution of the DNA obtained from five J2. The results showed that a specific and sensitive H. latipons species-specific PCR was constructed.

Genetic and pathogenic variation in Heterodera latipons populations from Turkey

Nematology ◽  
2020 ◽  
Vol 23 (1) ◽  
pp. 47-56
Author(s):  
Abdelfattah A. Dababat ◽  
Mustafa İmren ◽  
Göksel Özer ◽  
Fouad Mokrını ◽  
Nagihan Duman ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
The Other ◽  
Yield Losses ◽  
Barley Cultivars ◽  
Plant Parasite ◽  
Pathogenic Variation ◽  
The World ◽  
Heterodera Latipons ◽  
Moderately Resistant ◽  
Different Levels

Summary The cereal cyst nematode, Heterodera latipons, is an important plant parasite causing substantial yield losses in wheat throughout the world. This study aimed to determine genetic and pathogenic variation in H. latipons populations obtained from the southern part of Turkey. The populations were identified as H. latipons by sequencing the ITS-rDNA region and further sequence analysis showed an intraspecific genetic variation in H. latipons populations, which were clustered into different groups. The International Test Assortment materials were used to determine pathogenic variation (pathotypes) in these populations. The results showed that ‘Ortolan’, ‘Morocco’, ‘KVL191’, ‘Bajo Aragon 1-1’, ‘Herta’, ‘Martin 403-2’, ‘Sun II’ and ‘Pusa Hybrid Bsi’ cultivars were resistant or moderately resistant to the tested nematode populations. ‘Emir’, ‘Dalmatische’ and ‘Capa’ were susceptible to H. latipons populations. The Hatay population of H. latipons was detected as the most virulent nematode population because ten out of 20 cultivars were susceptible or moderately susceptible to this population. The least virulent population was the Kilis population, which caused susceptible reaction on six out of all cultivars with different levels. Based on this scheme, the Turkish populations were in the Ha1 group: the reactions of barley, oats and wheat classified them as either Ha41 or Ha51. Barley ‘KVL191’ was resistant to all nematode populations but susceptible to Ha51, and the reactions of the other barley cultivars were also consistent with the Turkish populations being Ha51. ‘AUS10894’ was susceptible to three nematode populations but resistant to Ha41, and the reaction of ‘Capa’ was also consistent with the Turkish populations being Ha51. However, the degree of susceptibility of all wheat differentials distinguishes the Turkish populations from other pathotypes in the Ha1 group.

Hatching behaviour and life cycle of Heterodera latipons Franklin under Syrian agro-ecological conditions

Nematology ◽  
2004 ◽  
Vol 6 (2) ◽  
pp. 245-256 ◽  
Author(s):  
Uwe Scholz ◽  
And Richard Sikora
Keyword(s):  
Life Cycle ◽  
Eastern Mediterranean ◽  
Cropping Systems ◽  
Tap Water ◽  
Mediterranean Area ◽  
Barley Root ◽  
Ecological Zones ◽  
Significant Difference ◽  
Soil Temperatures ◽  
Heterodera Latipons

AbstractThe cereal cyst nematode Heterodera latipons Franklin is widespread in the eastern Mediterranean area and often occurs in mixed populations with H. avenae in cereal cropping systems. Differences between the two species in hatching behaviour and development cycle may be factors responsible for the low level of detection of H. latipons in the region. The aim of this study was to investigate the influence of different temperature regimes, agro-ecosystem, sampling date, cyst age and barley root diffusate on the hatching behaviour of H. latipons and its life cycle. The investigations were conducted by observing hatch and development under both field and glasshouse conditions. The optimum temperature for hatching was 10°C. The hatched second-stage juveniles of H. latipons from the dry and wet agro-ecological zones are found in the soil from December to April with peak densities in January and February. Thirty to 45% of the eggs of both populations from the dry and wet zone hatch slowly over the growing season. The results of hatching tests, simulating pre-planting soil temperatures in Syria, indicate that treatment for 16 days at 5°C initiates the hatching cycle of H. latipons. There was no significant difference in total hatch when cysts were placed in tap water or in barley root diffusates.

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