scholarly journals Primary human mesothelial cell culture in the evaluation of the inflammatory response to different sclerosing agents used for pleurodesis

2021 ◽  
Vol 9 (8) ◽  
Author(s):  
Michal Mierzejewski ◽  
Magdalena Paplinska‐Goryca ◽  
Piotr Korczynski ◽  
Rafal Krenke
Keyword(s):  
Cell Culture ◽  
Inflammatory Response ◽  
Mesothelial Cell ◽  
Sclerosing Agents ◽  
Human Mesothelial Cell

Effect of diclofenac and antidepressants on the inflammatory response in astrocyte cell culture

2013 ◽  
Vol 21 (6) ◽  
pp. 421-425 ◽  
Author(s):  
Md. Mamun Al-Amin ◽  
Mir Muhammad Nasir Uddin ◽  
Md. Mahbubur Rahman ◽  
Hasan Mahmud Reza ◽  
Md. Sohel Rana
Keyword(s):  
Cell Culture ◽  
Inflammatory Response

Biocompatibility of a Bicarbonate/Lactate-Buffered PD Fluid Tested with a Double-Chamber Cell Culture System

2005 ◽  
Vol 25 (4) ◽  
pp. 387-393 ◽  
Author(s):  
Andreas Fusshoeller ◽  
Jessica Baehr ◽  
Bernd Grabensee ◽  
Joerg Plum
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Culture System ◽  
Mesothelial Cell ◽  
In Vitro Studies ◽  
Cell Culture System ◽  
And Function ◽  
Tgf Β1 ◽  
Double Chamber

Objective In peritoneal dialysis (PD), neutrally buffered PD fluids with lower concentrations of glucose degradation products (GDP) have tested superior to conventional fluids in terms of biocompatibility. However, conventional in vitro studies provoke debate because, due to the lack of subsequent equilibration with the blood, they do not resemble the true intraperitoneal situation of PD. Methods We established a double-chamber cell culture system with peritoneal mesothelial cells seeded on top of a permeable membrane, with a physiological buffer below. Thus adequately reflecting the in vivo equilibration pattern, we compared a conventional fluid with a neutral bicarbonate/lactate-buffered PD solution. Using an exchange pattern adapted from an 8-hour continuous ambulatory PD regimen, cell viability was assessed with an MTT assay, and cell function via constitutive and stimulated interleukin (IL)-6 release. As an indicator of potential induction of fibrosis and as a parameter of mesothelial cell integrity, respectively, transforming growth factor-beta 1 (TGF-β1) generation and cancer antigen 125 (CA125) release were measured. Results The conventional solution significantly compromised mesothelial cell viability and function in terms of mitochondrial activity ( p < 0.05) and stimulated IL-6 release ( p < 0.05). The bicarbonate/lactate fluid had no effect on cell viability or IL-6 release and turned out to be equivalent to the properties of the growth medium. Whereas lactate-incubated cells did not respond to IL-1β stimulation, bicarbonate/lactate-treated cells adequately increased IL-6 release after stimulation ( p < 0.0005). Release of TGF-β1 and CA125 did not differ between the different fluids and the control. Conclusions Due to the sustained equilibration process, the double-chamber cell culture model allows a more realistic insight into mesothelial cell viability and function in terms of PD. As in classic in vitro studies, an adverse effect of conventional PD solutions on mesothelial cells was overt in the present cell culture system. The neutral bicarbonate/lactate-buffered fluid with low GDP content, however, did not interfere with mesothelial cell vitality or function, indicating superior biocompatibility.

scholarly journals The Effects of Insufflation Conditions on Rat Mesothelium

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Andrew K. Davey ◽  
Jessica Hayward ◽  
Jean K. Marshall ◽  
Anthony E. Woods
Keyword(s):  
Inflammatory Response ◽  
Mesothelial Cell ◽  
Surface Group ◽  
Control Group ◽  
Cell Nuclei ◽  
Tissue Samples ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2at pressures equivalent to intraperitoneal pressures used in human laparoscopy.Methods. Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2. The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h. Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy (SEM).Results. Group 1: no abnormalities were detected. Group 2: specimens revealed an inflammatory response with loss of mesothelium and mesothelial cell nuclei showing lytic change. Cells were rounded with some areas of cell flattening and separation. Group 3: some animals showed little or no alteration, while others had a mild inflammatory response. Mesothelial cells were rounded and showed crenation on the exposed surface. Group 4: specimens showed little change from the control group.Conclusions. The LM results indicate that insufflations with heated, humidified CO2are the least likely to induce mesothelial damage.

Tumorigenicity of Human Mesothelial Cell Line Transfected With EJ-ras Oncogene

1989 ◽  
Vol 81 (12) ◽  
pp. 945-948 ◽  
Author(s):  
R. R. Reddel ◽  
L. Malan-Shibley ◽  
B. I. Gerwin ◽  
R. A. Metcalf ◽  
C. C. Harris
Keyword(s):  
Cell Line ◽  
Mesothelial Cell ◽  
Ras Oncogene ◽  
Human Mesothelial Cell

scholarly journals Different Cellular Response of Human Mesothelial Cell MeT-5A to Short-Term and Long-Term Multiwalled Carbon Nanotubes Exposure

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Li Ju ◽  
Wei Wu ◽  
Min Yu ◽  
Jianlin Lou ◽  
Hao Wu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cell Motility ◽  
Multiwalled Carbon Nanotubes ◽  
Mesothelial Cell ◽  
Continuous Exposure ◽  
Short Term ◽  
Multiwalled Carbon ◽  
Long Term Treatment ◽  
Human Mesothelial Cell

Despite being a commercially important product, multiwalled carbon nanotubes (MWCNTs) continue to raise concerns over human health due to their structural similarity to asbestos. Indeed, exposure to MWCNT has been shown to induce lung cancer and even mesothelioma, but contradictory results also exist. To clarify the potentially carcinogenic effects of rigid and rod-like MWCNT and to elucidate the underlying mechanisms, the effects of MWCNT on human mesothelial cell MeT-5A were examined throughout 3 months of continuous exposure, including cytotoxicity, genotoxicity, and cell motility. It was found that MWCNT did not affect MeT-5A cell proliferation at 10 μg/cm2 within 72 h treatment, but under the same condition, MWCNT induced genotoxicity and perturbed cell motility. In addition, MeT-5A cells demonstrated different cellular responses to MWCNT after short-term and long-term exposure. Taken together, our results indicated a possible carcinogenic potential for MWCNT after long-term treatment, in which Annexin family proteins might be involved.

Peritoneal Mesothelial Cell Culture and Biology

2006 ◽  
Vol 26 (2) ◽  
pp. 162-193 ◽  
Author(s):  
Susan Yung ◽  
Fu Keung Li ◽  
Tak Mao Chan
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Immunohistochemical Markers ◽  
Tumor Dissemination ◽  
Structural And Functional Properties ◽  
Peritoneal Mesothelium ◽  
Peritoneal Mesothelial Cell

The peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body's serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the peritoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications.

scholarly journals Experimental study on the regulation of the cholinergic pathway in renal macrophages by microRNA-132 to alleviate inflammatory response

2018 ◽  
Vol 16 (1) ◽  
pp. 176-183
Author(s):  
Ming Wu ◽  
Nana Li ◽  
Ji Xu ◽  
Lefeng Wu ◽  
Mingli Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Inflammatory Response ◽  
Hemorrhagic Shock ◽  
Culture System ◽  
Kidney Injury ◽  
Glucose Deprivation ◽  
Stable Expression ◽  
Inflammatory Factors ◽  
Cell Culture System ◽  
Expression Levels

AbstractMicroRNA-132 (miR-132) is correlated with inflammatory response regulation, although its effect on acute kidney injury to provide protection against hemorrhagic shock remains currently unknown. AChE in macrophages of the kidney subjected under hemorrhagic shock is presumed to be regulated by miR-132 after its transcription to alleviate the inflammatory response accordingly. Antagonists such as acetylcholine (Ach) (concentration 10−4mol/L) and galanthamine (Gal) (concentration 10μmol/L) were added into separate groups 1 hour after the macrophages in the kidney were isolated and cultured to induce injury under oxygen and glucose deprivation (OGD) and then cultured for 24 hours. To analyze the effect of miR-132, we placed the renal epithelial cells transfected with miR-132 plasmids with stable expression over the renal macrophages to create a double cell culture system. The expression levels of inflammatory factors and apoptosis under OGD were significantly higher in renal macrophages than in other experimental groups. Moreover, the expression of miR-132 in macrophages of the double cell culture system showing stable expression of miR-132 increased, whereas that of several inflammatory factors was significantly inhibited. The expression levels of AChE mRNA and protein in the macrophages significantly decreased. The cholinergic antiinflammatory pathway in renal macrophages is regulated by miR-132 via inhibition of the hydrolytic activity of cholinesterase to alleviate inflammatory response, which may play a role in the prevention and treatment of kidney injury caused by hemorrhagic shock.

scholarly journals Chlamydia trachomatis Infection of Human Mesothelial Cells Alters Proinflammatory, Procoagulant, and Fibrinolytic Responses

1998 ◽  
Vol 66 (5) ◽  
pp. 2352-2355 ◽  
Author(s):  
Mireille van Westreenen ◽  
Apollo Pronk ◽  
Rob J. A. Diepersloot ◽  
Philip G. de Groot ◽  
Piet Leguit
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydial Infection ◽  
Mesothelial Cell ◽  
Plasminogen Activator Inhibitor ◽  
Plasminogen Activator Inhibitor 1 ◽  
Initial Infection ◽  
Chlamydia Trachomatis Infection ◽  
Proinflammatory Responses ◽  
Human Mesothelial Cell ◽  
Activator Inhibitor

ABSTRACT In this study we demonstrate the capability of Chlamydia trachomatis to infect cultured human mesothelial cell (MC) monolayers and to induce the production of the proinflammatory cytokines interleukin 1β (IL-1β) and IL-8. Seventy-two hours after initial infection, both the procoagulant activity of MC and the activity of the fibrinolytic inhibitor (plasminogen activator inhibitor 1) in the supernatants were enhanced. These findings support the hypothesis that provoked proinflammatory responses contribute to the development of complications after chlamydial infection.

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