scholarly journals A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina

2018 ◽  
Vol 6 (20) ◽  
pp. e13885
Author(s):  
Chase B. Hellmer ◽  
Melissa Rampino Clemons ◽  
Scott Nawy ◽  
Tomomi Ichinose
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Bipolar Cells ◽  
Mouse Retina ◽  
Metabotropic Glutamate ◽  
Group I ◽  
Synaptic Gain ◽  
Rod Bipolar Cells

Are there ionotropic glutamate receptors on the rod bipolar cell of the mouse retina?

1997 ◽  
Vol 14 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Thomas E. Hughes
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Bipolar Cell ◽  
Metabotropic Glutamate Receptor ◽  
Bipolar Cells ◽  
Ionotropic Glutamate Receptors ◽  
Mouse Retina ◽  
Rod Photoreceptor ◽  
Metabotropic Glutamate ◽  
C Terminus

AbstractThere is some evidence that the mammalian rod bipolar cell expresses ionotropic glutamate receptors. This is surprising in light of the strong evidence that the glutamate released by the rod photoreceptor acts upon a metabotropic glutamate receptor-mGluRo-present in the dendrites of the rod bipolar cell. To reexamine the issue of which glutamate receptor subunits may be present on the rod bipolar cell, an immunohistochemical study of acutely dissociated retinal cells was undertaken. Two monoclonal antibodies provided some evidence that GluR2 and/or GluR4, as well as NMDAR1 subunit, are present on the rod bipolar cell. A monoclonal antibody directed against the N-terminus of GluR2 labeled the rod bipolar cells, but two antisera directed against the C-terminus of the same subunit did not. One possible explanation for this discrepancy could be that the rare splice variant GluR2-long, which is endowed with a different C-terminus, could be expressed by the rod bipolar cell. To explore this possibility, RT-PCR was used to amplify the transcripts encoding GluR2 in the neural retina. This revealed that GluR2-long transcripts, with the flop exon, are present.

Metabotropic Glutamate Receptor Agonists Alter Neuronal Excitability and Ca2+ Levels via the Phospholipase C Transduction Pathway in Cultured Purkinje Neurons

1997 ◽  
Vol 78 (1) ◽  
pp. 63-75 ◽  
Author(s):  
Jeffrey G. Netzeband ◽  
Kathy L. Parsons ◽  
Dan D. Sweeney ◽  
Donna L. Gruol
Keyword(s):  
Phospholipase C ◽  
Glutamate Receptor ◽  
Neuronal Excitability ◽  
Metabotropic Glutamate Receptor ◽  
Purkinje Neurons ◽  
Transduction Pathway ◽  
Electrophysiological Response ◽  
Metabotropic Glutamate ◽  
Group I ◽  
Intracellular Ca

Netzeband, Jeffrey G., Kathy L. Parsons, Dan D. Sweeney, and Donna L. Gruol. Metabotropic glutamate receptor agonists alter neuronal excitability and Ca2+ levels via the phospholipase C transduction pathway in cultured Purkinje neurons. J. Neurophysiol. 78: 63–75, 1997. Selective agonists for metabotropic glutamate receptor (mGluR) subtypes were tested on mature, cultured rat cerebellar Purkinje neurons (≥21 days in vitro) to identify functionally relevant mGluRs expressed by these neurons and to investigate the transduction pathways associated with mGluR-mediated changes in membrane excitability. Current-clamp recordings (nystatin/perforated-patch method) were used to measure the membrane response of Purkinje neurons to brief microperfusion pulses (1.5 s) of the group I (mGluR1/mGluR5) agonists (1 S,3 R)-1-aminocyclopentane-1,3-dicarboxylic acid (300 μM), quisqualate (5 μM), and ( R,S)-3,5-dihydroxyphenylglycine (50–500 μM). All group I mGluR agonists elicited biphasic membrane responses and burst activity in the Purkinje neurons. In addition, the group I mGluR agonists produced alterations in the active membrane properties of the Purkinje neurons and depressed the off response after hyperpolarizing current injection. In parallel microscopic Ca2+ imaging experiments, application of the group I mGluR agonists to fura-2-loaded cells elicited increases in intracellular Ca2+ in both the somatic and dendritic regions. The group II (mGluR2/mGluR3) agonist (2 S,3 S,4 S)-α-(carboxycyclopropyl)-glycine (10 μM) and the group III (mGluR4/mGluR6/mGluR7/mGluR8) agonists l(+)-2-amino-4-phosphonobutyric acid (1 mM) and O-phospho-l-serine (200 μM) had no effect on the membrane potential or intracellular Ca2+ levels of the Purkinje neurons. The cultured Purkinje neurons, but not granule neurons or interneurons, showed immunostaining for mGluR1α in both the somatic and dendritic regions. All effects of the group I mGluR agonists were blocked by (+)-α-methyl-4-carboxyphenylglycine (1 mM), an mGluR antagonist. Furthermore, the phospholipase C inhibitor 1-[6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-1H-pyrrole-2,5-dione (2 μM) blocked the group I mGluR agonist-mediated electrophysiological response and greatly attenuated the Ca2+ signal elicited by group I mGluR agonists, particularly in the dendrites. The inactive analogue1-[6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]2,5-pyrrolidine-dione (2 μM) was relatively ineffective against the electrophysiological response and Ca2+ signal. These results indicate that functional group I mGluRs (but not group II or III mGluRs) can be activated on mature Purkinje neurons in culture and result in changes in neuronal excitability and intracellular Ca2+ mediated through phospholipase C. These data obtained from a defined neuronal type, the Purkinje neuron, confirm biochemical and molecular studies on the transduction mechanisms of group I mGluRs and show that this transduction pathway is linked to neuronal excitability and intracellular Ca2+ release in the Purkinje neurons.

Group I Metabotropic Glutamate Receptor-mediated Gene Expression in Striatal Neurons

2008 ◽  
Vol 33 (10) ◽  
pp. 1920-1924 ◽  
Author(s):  
Li-Min Mao ◽  
Guo-Chi Zhang ◽  
Xian-Yu Liu ◽  
Eugene E. Fibuch ◽  
John Q. Wang
Keyword(s):  
Gene Expression ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Striatal Neurons ◽  
Metabotropic Glutamate ◽  
Group I

Orai1 is a crucial downstream partner of group I metabotropic glutamate receptor signaling in dorsal horn neurons

Pain ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Jingsheng Xia ◽  
Yannong Dou ◽  
Yixiao Mei ◽  
Frances M. Munoz ◽  
Ruby Gao ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Receptor Signaling ◽  
Metabotropic Glutamate ◽  
Dorsal Horn Neurons ◽  
Group I
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