scholarly journals Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle

2018 ◽  
Vol 6 (17) ◽  
pp. e13868 ◽  
Author(s):  
Daniil V. Popov ◽  
Evgeny A. Lysenko ◽  
Roman O. Bokov ◽  
Maria A. Volodina ◽  
Nadia S. Kurochkina ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Aerobic Training ◽  
Respiratory Proteins

scholarly journals Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle

2004 ◽  
Vol 557 (2) ◽  
pp. 559-570 ◽  
Author(s):  
Paul J. LeBlanc ◽  
Sandra J. Peters ◽  
Rebecca J. Tunstall ◽  
David Cameron-Smith ◽  
George J. F. Heigenhauser
Keyword(s):  
Skeletal Muscle ◽  
Pyruvate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Aerobic Training ◽  
Pyruvate Dehydrogenase Kinase

scholarly journals PL-008 Adaptation of skeletal muscle to aerobic exercise: specific transcriptome response to acute exercise and training

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Daniil Popov ◽  
Pavel Makhnovskii ◽  
Evgeny Lysenko ◽  
Olga Vinogradova
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Circadian Rhythm ◽  
Human Skeletal Muscle ◽  
Acute Exercise ◽  
Aerobic Training ◽  
Contractile Activity ◽  
Training Programme ◽  
Specific Response ◽  
Transcriptome Response

Objective Variety of processes including circadian rhythm and systemic factors affect expression of many genes in skeletal muscle during a day. Therefore, post-exercise gene expression depends on many factors: contractile activity per seas well as circadian rhythm, nerve activity, concentration of different substances in blood, feeding and fasting. In our study, we investigated specific for contractile activity changes in the transcriptome in untrained and trained (after an aerobic training programme) human skeletal muscle. The second goal was to examine effect of aerobic training on gene expression in muscle in basal state. Methods Seven untrained males performed the one-legged knee extension exercise (for 60 min) with the same relative intensity before and after a 2 month aerobic training programme (1 h/day, 5/week). Biopsy samples were taken at rest (basal state, 48 h after the previous exercise), 1 and 4 h after one-legged exercise from m. vastus lateralisof either leg. This approach allowed us to evaluate specific changes in the transcriptome associated with contractile activity. RNA­sequencing (84 samples in total; ~42 million reads/sample) was performed by HiSeq 2500 (Illumina). Results Two months aerobic training increased the aerobic capacity of the knee-extensor muscles (power at anaerobic threshold in incremental one-legged and cycling tests), the maximum rate of ADP-stimulated mitochondrial respiration in permeabilized muscle fibres and amounts of oxidative phosphorylation proteins. After one-legged exercise, expression of many genes was changed in exercised muscle (~1500) as well as in non-exercised muscle (~400). Pronounced changes in gene expression in non-exercised muscle may be associated with many factors, including circadian rhythm (result of GO analysis). To examine transcriptome changes specific for contractile activity, the difference in gene expression between legs was examined. In untrained muscle, one-legged exercise changed expression of ~1200 genes specific for contractile activity at each time point. Despite the same relative intensity of one-legged exercise, transcriptomic response in trained muscle was markedly lower (~300 genes) compare to untrained. We observed a strong overlap between transcriptomic responses (~250 genes) and particularly between enriched transcription factor binding sites in promoters of these genes in untrained and trained muscles. These sets of genes and transcription factors play the key role in adaptation of muscle to contractile activity independently on the level of muscular fitness. Surprisingly, 2 months aerobic training changed the expression of more than 1500 genes in basal state. Noteworthy, these genes demonstrated a small overlap (~200 genes) with genes related to specific response to acute exercise. Moreover, these genes were associated with significantly different biological processes than genes related to specific response to acute exercise. Conclusions Specific for contractile activity changes in the transcriptome in untrained and trained human skeletal muscle were revealed for the first time. After 2 month aerobic training, the specific transcriptome response to acute exercise become much less pronounced. A computational approach reveals common transcription factors important for adaptation of both untrained and trained muscle. We found out that adaptation of muscle to aerobic training associates not only with the transitory changes in gene expression after each exercise, but also with the marked changes in transcriptome in basal state. This work was supported by the Russian Science Foundation (14­15­00768).

Sensitivity of CPT I to malonyl-CoA in trained and untrained human skeletal muscle

2000 ◽  
Vol 278 (3) ◽  
pp. E462-E468 ◽  
Author(s):  
Emma C. Starritt ◽  
Richard A. Howlett ◽  
George J. F. Heigenhauser ◽  
Lawrence L. Spriet
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Aerobic Training ◽  
Fat Metabolism ◽  
Acetyl Coa ◽  
Malonyl Coa ◽  
Metabolic Conditions ◽  
Carnitine Palmitoyltransferase I ◽  
Cpt I ◽  
Rest And Exercise

The present study examined the sensitivity of carnitine palmitoyltransferase I (CPT I) activity to its inhibitor malonyl-CoA (M-CoA), and simulated metabolic conditions of rest and exercise, in aerobically trained and untrained humans. Maximal CPT I activity was measured in mitochondria isolated from resting human skeletal muscle. Mean CPT I activity was 492.8 ± 72.8 and 260.8 ± 33.6 μmol ⋅ min−1 ⋅ kg wet muscle−1 in trained and untrained subjects, respectively (pH 7.0, 37°C). The sensitivity to M-CoA was greater in trained muscle; the IC50 for M-CoA was 0.17 ± 0.04 and 0.49 ± 0.17 μM in trained and untrained muscle, respectively. The presence of acetyl-CoA, free coenzyme A (CoASH), and acetylcarnitine, in concentrations simulating rest and exercise conditions did not release the M-CoA-induced inhibition of CPT I activity. However, CPT I activity was reduced at pH 6.8 vs. pH 7.0 in both trained and untrained muscle in the presence of physiological concentrations of M-CoA. The results of this study indicate that aerobic training is associated with an increase in the sensitivity of CPT I to M-CoA. Accumulations of acetyl-CoA, CoASH, and acetylcarnitine do not counteract the M-CoA-induced inhibition of CPT I activity. However, small decreases in pH produce large reductions in the activity of CPT I and may contribute to the decrease in fat metabolism that occurs during moderate and intense aerobic exercise intensities.

Effect of l-carnitine supplementation and aerobic training on FABPc content and β-HAD activity in human skeletal muscle

2006 ◽  
Vol 99 (2) ◽  
pp. 193-199 ◽  
Author(s):  
Jang Kyu Lee ◽  
Jong Sam Lee ◽  
Hyon Park ◽  
Youn-Soo Cha ◽  
Chung Su Yoon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Aerobic Training ◽  
Carnitine Supplementation

TGFß regulates metabolism of human skeletal muscle cells by miRNAs

2018 ◽  
Author(s):  
S Höckele ◽  
P Huypens ◽  
C Hoffmann ◽  
T Jeske ◽  
M Hastreiter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

IRAK-1/IRS-1 Signaling Cross Talk in Human Skeletal Muscle—Role in Insulin Resistance

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 159-OR
Author(s):  
THEODORE P. CIARALDI ◽  
SUNDER MUDALIAR ◽  
LIWU LI ◽  
ROSARIO SCALIA ◽  
XIAO JIAN SUN ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Cross Talk ◽  
Human Skeletal Muscle

1891-P: Palmitate-Enriched Lipid Ingestion Acutely Induces Insulin Resistance Associated with PKCγ Activation and Impaired Mitochondrial Function in Human Skeletal Muscle

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1891-P
Author(s):  
THERESIA SARABHAI ◽  
CHRYSI KOLIAKI ◽  
SABINE KAHL ◽  
DOMINIK PESTA ◽  
LUCIA MASTROTOTARO ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Mitochondrial Function ◽  
Human Skeletal Muscle
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