scholarly journals Gestation changes sodium pump isoform expression, leading to changes in ouabain sensitivity, contractility, and intracellular calcium in rat uterus

2017 ◽  
Vol 5 (23) ◽  
pp. e13527 ◽  
Author(s):  
Rachel V. Floyd ◽  
Ali Mobasheri ◽  
Susan Wray
Keyword(s):  
Intracellular Calcium ◽  
Sodium Pump ◽  
Rat Uterus ◽  
Isoform Expression ◽  
Ouabain Sensitivity

Effect of ouabain on sodium pump alpha-isoform expression in an animal model of mania

2009 ◽  
Vol 33 (7) ◽  
pp. 1103-1106 ◽  
Author(s):  
Humera Hamid ◽  
YongLin Gao ◽  
Zhenmin Lei ◽  
M. Tyler Hougland ◽  
Rif S. El-Mallakh
Keyword(s):  
Animal Model ◽  
Sodium Pump ◽  
Isoform Expression

Isoform expression of Na+-K+-ATPase α-subunit in gills of the teleostOreochromis mossambicus

1998 ◽  
Vol 275 (3) ◽  
pp. R926-R932 ◽  
Author(s):  
Tsung-Han Lee ◽  
Jong-Chang Tsai ◽  
Mei-Jane Fang ◽  
Ming-Jiun Yu ◽  
Pung-Pung Hwang
Keyword(s):  
Image Analysis ◽  
Sodium Pump ◽  
Gill Tissue ◽  
Oreochromis Mossambicus ◽  
Acid Base ◽  
Α Subunit ◽  
Specific Antibodies ◽  
Gill Mitochondria ◽  
Isoform Expression ◽  
Α Subunits

Three isoform-specific antibodies, 6F against the α1-isoform of the avian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α-subunits in gills of a teleost, the tilapia ( Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3-isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia.

Enhanced ouabain sensitivity of the heart and myocardial sodium pump in aged rats

1984 ◽  
Vol 105 (1-2) ◽  
pp. 95-103 ◽  
Author(s):  
Yumi Katano ◽  
Tai Akera ◽  
Kyosuke Temma ◽  
Richard H. Kennedy
Keyword(s):  
Sodium Pump ◽  
Aged Rats ◽  
Ouabain Sensitivity

Sodium pump isoform expression in heart failure: implication for treatment

2002 ◽  
Vol 97 (7) ◽  
pp. 1-1 ◽  
Author(s):  
Jochen M�ller-Ehmsen ◽  
Robert A. Farley ◽  
Robert H. G. Schwinger ◽  
Alicia A. McDonough
Keyword(s):  
Heart Failure ◽  
Sodium Pump ◽  
Isoform Expression

Phospholipid Scramblase Isoform Expression in Pregnant Rat Uterus

2006 ◽  
Vol 13 (7) ◽  
pp. 497-501 ◽  
Author(s):  
Mark Phillippe ◽  
Diana F. Bradley ◽  
Huiling Ji ◽  
Karen H. Oppenheimer ◽  
Edward K. Chien
Keyword(s):  
Rat Uterus ◽  
Pregnant Rat ◽  
Phospholipid Scramblase ◽  
Isoform Expression

Ultra-Rapid Freezing of Isolated Skeletal Muscle Fibers

1977 ◽  
Vol 35 ◽  
pp. 576-577
Author(s):  
Joachim R. Sommer ◽  
Nancy R. Wallace
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Granular Material ◽  
Nuclear Envelope ◽  
Intracellular Calcium ◽  
Ruthenium Red ◽  
Threshold Concentration ◽  
Rapid Freezing ◽  
Frog Skeletal Muscle ◽  
Electrical Isolation

After Howell (1) had shown that ruthenium red treatment of fixed frog skeletal muscle caused collapse of the intermediate cisternae of the sarcoplasmic reticulum (SR), forming a pentalaminate structure by obi iterating the SR lumen, we demonstrated that the phenomenon involves the entire SR including the nuclear envelope and that it also occurs after treatment with other cations, including calcium (2,3,4).From these observations we have formulated a hypothesis which states that intracellular calcium taken up by the SR at the end of contraction causes the M rete to collapse at a certain threshold concentration as the first step in a subsequent centrifugal zippering of the free SR toward the junctional SR (JSR). This would cause a) bulk transport of SR contents, such as calcium and granular material (4) into the JSR and, b) electrical isolation of the free SR from the JSR.

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