scholarly journals Nanoparticles increase human bronchial epithelial cell susceptibility to respiratory syncytial virus infection via nerve growth factor-induced autophagy

2017 ◽  
Vol 5 (13) ◽  
pp. e13344 ◽  
Author(s):  
Sreeparna Chakraborty ◽  
Vincent Castranova ◽  
Miriam K. Perez ◽  
Giovanni Piedimonte
Keyword(s):  
Respiratory Syncytial Virus ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Virus Infection ◽  
Respiratory Syncytial Virus Infection ◽  
Bronchial Epithelial Cell ◽  
Bronchial Epithelial ◽  
Nerve Growth ◽  
Syncytial Virus

scholarly journals Identification of gene biomarkers for respiratory syncytial virus infection in a bronchial epithelial cell line

2008 ◽  
Vol 2 (3-4) ◽  
pp. 113-125 ◽  
Author(s):  
Yuh-Chin T. Huang ◽  
Zhuowei Li ◽  
Xhevahire Hyseni ◽  
Michael Schmitt ◽  
Robert B. Devlin ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Epithelial Cell ◽  
Virus Infection ◽  
Cell Line ◽  
Respiratory Syncytial Virus Infection ◽  
Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Bronchial Epithelial ◽  
Syncytial Virus

scholarly journals Comparative Therapeutic Potential of ALX-0171 and Palivizumab against Respiratory Syncytial Virus Clinical Isolate Infection of Well-Differentiated Primary Pediatric Bronchial Epithelial Cell Cultures

2019 ◽  
Vol 64 (2) ◽  
Author(s):  
Lindsay Broadbent ◽  
Hong Guo Parke ◽  
Lyndsey J. Ferguson ◽  
Andrena Millar ◽  
Michael D. Shields ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Epithelial Cell ◽  
Clinical Isolate ◽  
Therapeutic Potential ◽  
Bronchial Epithelial Cell ◽  
Bronchial Epithelial ◽  
Young Infants ◽  
Syncytial Virus ◽  
Well Differentiated ◽  
Tract Infections

ABSTRACT Respiratory syncytial virus (RSV) causes severe lower respiratory tract infections in young infants. There are no RSV-specific treatments available. Ablynx has been developing an anti-RSV F-specific nanobody, ALX-0171. To characterize the therapeutic potential of ALX-0171, we exploited our well-differentiated primary pediatric bronchial epithelial cell (WD-PBEC)/RSV infection model, which replicates several hallmarks of RSV disease in vivo. Using 2 clinical isolates (BT2a and Memphis 37), we compared the therapeutic potential of ALX-0171 with that of palivizumab, which is currently prescribed for RSV prophylaxis in high-risk infants. ALX-0171 treatment (900 nM) at 24 h postinfection reduced apically released RSV titers to near or below the limit of detection within 24 h for both strains. Progressively lower doses resulted in concomitantly diminished RSV neutralization. ALX-0171 was approximately 3-fold more potent in this therapeutic RSV/WD-PBEC model than palivizumab (mean 50% inhibitory concentration [IC50] = 346.9 to 363.6 nM and 1,048 to 1,090 nM for ALX-0171 and palivizumab, respectively), irrespective of the clinical isolate. The number of viral genomic copies (GC) was determined by quantitative reverse transcription-PCR (RT-qPCR), and the therapeutic effect of ALX-0171 treatment at 300 and 900 nM was found to be considerably lower and the number of GCs reduced only moderately (0.62 to 1.28 log10 copies/ml). Similar findings were evident for palivizumab. Therefore, ALX-0171 was very potent at neutralizing RSV released from apical surfaces but had only a limited impact on virus replication. The data indicate a clear disparity between viable virus neutralization and GC viral load, the latter of which does not discriminate between viable and neutralized RSV. This report validates the RSV/WD-PBEC model for the preclinical evaluation of RSV antivirals.

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