Anti-allergic Effects and Immunomodulatory Activity of Sacran, a Bioactive Compound from River alga Aphanothece sacrum

2018 ◽  
Vol 2 ◽  
Keyword(s):  
Bioactive Compound ◽  
Immunomodulatory Activity

scholarly journals Mining for Active Molecules in Probiotic Supernatant by Combining Non-Targeted Metabolomics and Immunoregulation Testing

Metabolites ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 35
Author(s):  
Juliano Roldan Fonseca ◽  
Marianna Lucio ◽  
Mourad Harir ◽  
Philippe Schmitt-Kopplin
Keyword(s):  
Metabolic Pathway ◽  
Solid Phase ◽  
Bioactive Compound ◽  
Immunomodulatory Activity ◽  
Host Immune System ◽  
Multivariate Statistical ◽  
Chronic Respiratory Diseases ◽  
Molecular Features ◽  
Negative Ionization

Chronic respiratory diseases such as asthma are highly prevalent in industrialized countries. As cases are expected to rise, there is a growing demand for alternative therapies. Our recent research on the potential benefits of probiotics suggests that they could prevent and reduce the symptoms of many diseases by modulating the host immune system with secreted metabolites. This article presents the first steps of the research that led us to identify the immunoregulatory bioactivity of the amino acid d-Trp reported in our previous study. Here we analyzed the cell culture metabolic footprinting of 25 commercially available probiotic strains to associate metabolic pathway activity information with their respective immune modulatory activity observed in vitro. Crude probiotic supernatant samples were processed in three different ways prior to untargeted analysis in positive and negative ionization mode by direct infusion ESI-FT-ICR-MS: protein precipitation and solid phase extraction (SPE) using HLB and CN-E sorbent cartridges. The data obtained were submitted to multivariate statistical analyses to distinguish supernatant samples into the bioactive and non-bioactive group. Pathway analysis using discriminant molecular features showed an overrepresentation of the tryptophan metabolic pathway for the bioactive supernatant class, suggesting that molecules taking part in that pathway may be involved in the immunomodulatory activity observed in vitro. This work showcases the potential of metabolomics to drive product development and novel bioactive compound discovery out of complex biological samples in a top-down manner.

scholarly journals Macrophage immunomodulatory activity of Acanthopanax senticousus polysaccharide nanoemulsion via activation of P65/JNK/ikkαsignaling pathway and regulation of Th1/Th2 Cytokines

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12575
Author(s):  
Xianghui Li ◽  
Zhiqiang Zhang ◽  
Zhenhuan Guo ◽  
Li Zhao ◽  
Yonglu Liu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Negative Charge ◽  
Bioactive Compound ◽  
Immunomodulatory Activity ◽  
Th2 Cytokines ◽  
Splenocyte Proliferation ◽  
Immunostimulatory Activity ◽  
Tnf Α ◽  
Ifn Γ ◽  
Immune Enhancement

Nanoemulsions (NE) are used widely in pharmaceutical drug formulations and vaccine preparation, and Acanthopanax senticousus polysaccharide (ASPS) is a natural bioactive compound with immunostimulatory activity. Therefore, NE-loaded ASPS is expected to provide immunological enhancement for effective treatment. In the present study, Acanthopanax senticousus polysaccharide (ASPS was encapsulated into nanoemulsions, the resultant ASPS–NE were coated with a negative charge, and the immune enhancement mechanism of these ASPS-NE formulations was analyzed. The immunosuppressive animal models (70 ICR mice, male) for the study were established using cyclophosphamide. In addition, the activation of splenocyte proliferation, phagocytosis of the macrophages, the ratio of CD4+ to CD8+, the concentrations of the cytokines in serum, Western blot analysis was used for the analysis of the P65/JNK/ikk α signaling pathway in the peritoneal macrophage s. The results revealed that the ASPS-NE could stimulated the proliferation of splenocytes and enhance immunity. The ASPS-NE induced the expression of different cytokines (TNF-α, IFN-γ, IL-2, and IL-6), could activate the expressions of P65, JNK, and ikkα, and regulated the Th1/Th2 cytokines. These findings demonstrated the potential of ASPS-NE formulations for drug delivery and to induce potent and sustained immune responses.

scholarly journals Immunomodulatory Effects of a Bioactive Compound Isolated fromDryopteris crassirhizomaon the Grass CarpCtenopharyngodon idella

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Cheng Chi ◽  
Sib Sankar Giri ◽  
Jin Woo Jun ◽  
Hyoun Joong Kim ◽  
Saekil Yun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Grass Carp ◽  
Head Kidney ◽  
Bioactive Compound ◽  
Ctenopharyngodon Idella ◽  
Complement C3 ◽  
Immunomodulatory Activity ◽  
Immune Parameters ◽  
Significant Expression ◽  
Immune Related Genes

In the present study, we investigated effects of compound kaempferol 3-a-L-(4-O-acetyl)rhamnopyranoside-7-a-L-rhamnopyranoside (SA) isolated fromDryopteris crassirhizomaduring immune-related gene expression inCtenopharyngodon idellahead kidney macrophages (CIHKM). The expression of immune-related genes (IL-1β,TNF-α,MyD88, andMx1) were investigated using real-time PCR at 2 h, 8 h, 12 h, and 24 h after incubation with 1, 10, and 50 μg mL−1of SA. Furthermore, fish were injected intraperitoneally with 100 μL of SA, and immune parameters such as lysozyme activity, complement C3, SOD, phagocytic activity, and IgM level were examined at 1, 2, and 3 weeks after injection. The differential expression of cytokines was observed after exposure to SA.IL-1βgenes displayed significant expression at 2 and 8 h after exposure to 1–10 μg mL−1of SA. SA also induced gene expression of cytokines such asMyD88,Mx1, andTNF-α. Furthermore, enhanced immune parameters in grass carp confirmed the immunomodulatory activity of SA. Interestingly, this compound has no toxic effect on CIHKM cells as tested by MTT assay. In addition, fish immunised with 10 μg mL−1of SA exhibited maximum resistance againstAeromonas hydrophilainfection. These results suggest that SA has the potential to stimulate immune responses in grass carp.

Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent

2013 ◽  
Vol 41 (01) ◽  
pp. 131-144 ◽  
Author(s):  
Shu-Jing Wu ◽  
Tzy-Ming Lu ◽  
Min-Nan Lai ◽  
Lean-Teik Ng
Keyword(s):  
Bioactive Compound ◽  
Grifola Frondosa ◽  
Immunomodulatory Activity ◽  
Medicinal Mushroom ◽  
Immune Functions ◽  
Traditional Medicines ◽  
Ergosterol Peroxide ◽  
Low Concentrations ◽  
Tnf Α ◽  
Full Protection

Grifola frondosa (GF), a high value medicinal mushroom in China and Japan, is popularly consumed as traditional medicines and health foods, especially for enhancing immune functions. In this study, our aim was to examine the immunomodulatory activities of GF and its bioactive compound ergosterol peroxide (EPO) in lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. At low concentrations, EPO but not other extracts showed a full protection against LPS-induced cell toxicity. EPO significantly blocked MyD88 and VCAM-1 expression, and cytokine (IL-1β, IL-6 and TNF-α) production in LPS-stimulated cells. It also effectively inhibited NF-κB activation, which was further confirmed with siRNA treatment. These results conclude that EPO may play an important role in the immunomodulatory activity of GF through inhibiting the production of pro-inflammatory mediators and activation of NF-κB signaling pathway.

Optimization of biomass production with enhanced bioactive compound content by the medicinal mushroom Ganoderma australe under submerged culture

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
LM Papaspyridi ◽  
E Topakas ◽  
N Aligiannis ◽  
P Christakopoulos ◽  
AL Skaltsounis ◽  
...  
Keyword(s):  
Biomass Production ◽  
Submerged Culture ◽  
Bioactive Compound ◽  
Medicinal Mushroom ◽  
Ganoderma Australe

Platelet Enhancing And Immunomodulatory Activity Of Carica Papaya Leaves

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
SH Ansari ◽  
V Anjum ◽  
S Ahmad
Keyword(s):  
Carica Papaya ◽  
Immunomodulatory Activity

Comparison of the Antioxidative Activities and Bioactive Compound Contents of Green Tea, Roasted Green Tea, and Black Tea

2018 ◽  
Vol 24 (3) ◽  
pp. 64-71 ◽  
Author(s):  
Eu-Jin Ban ◽  
Ju Hyung Kim ◽  
Gyu Yeon Oh ◽  
Su Yeon Lee ◽  
Jae-Hak Moon ◽  
...  
Keyword(s):  
Green Tea ◽  
Bioactive Compound ◽  
Black Tea

Immunomodulatory Activity of Pine Needle (Pinus densiflora) Extracts in Macrophages

2006 ◽  
Vol 11 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Hye-Sook Choi ◽  
Do Hang ◽  
Seong-Jun Cho ◽  
Se-Chan Kang ◽  
Eun-Soo Sohn ◽  
...  
Keyword(s):  
Pinus Densiflora ◽  
Immunomodulatory Activity ◽  
Pine Needle
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