scholarly journals Early mRNA Expression of Neuroendocrine Differentiation Signals Predicts Recurrence After Radical Prostatectomy: A Transcriptomic Analysis

2021 ◽  
Vol 12 (6) ◽  
pp. 232-239
Author(s):  
Panagiotis J. Vlachostergios ◽  
Athanasios Karathanasis ◽  
Christos N. Papandreou ◽  
Vassilios Tzortzis
Keyword(s):  
Radical Prostatectomy ◽  
Mrna Expression ◽  
Neuroendocrine Differentiation ◽  
Transcriptomic Analysis

scholarly journals Notch1-Hes1 signalling axis in the tumourigenesis of biliary neuroendocrine tumours

2013 ◽  
Vol 66 (5) ◽  
pp. 386-391 ◽  
Author(s):  
Kenichi Harada ◽  
Yasunori Sato ◽  
Hiroko Ikeda ◽  
Maylee Hsu ◽  
Saya Igarashi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Chromogranin A ◽  
Neuroendocrine Tumours ◽  
Neuroendocrine Differentiation ◽  
Biliary Tree ◽  
Neuroendocrine Marker ◽  
Cholangiocarcinoma Cell ◽  
Neoplastic Epithelium

AimsBiliary neuroendocrine tumours (NETs) are rare and mostly exist as a component of mixed adenoneuroendocrine carcinomas (MANECs). Although the NET component in biliary MANECs is generally more malignant and clinically more important to the prognosis than the ordinary adenocarcinomatous component, the histogenesis of biliary NET has not been clarified. In this study, the role of the Notch1-Hes1 signalling axis in the histogenesis of biliary NETs was examined.MethodsImmunohistochemistry for Notch1, its ligand Jagged1 and Hes1 was performed using surgical specimens from 11 patients with biliary MANEC. Moreover, after the knock-down of Notch1 mRNA expression in a cholangiocarcinoma cell line, the expression of chromogranin A (a neuroendocrine marker) and Ascl1 (a neuroendocrine-inducing molecule inhibited by activated Hes1) was examined by quantitative PCR.ResultsHistological examination revealed that the adenocarcinomatous components were predominately located at the luminal surface of the MANEC and the majority of stromal invasion involved NET components. Ordinary adenocarcinomas and non-neoplastic biliary epithelium constantly expressed Notch1, Jagged1 and Hes1, but the expression of Notch1 and Hes1 was decreased or absent in NET components, suggesting interference with the Notch1-Hes1 signalling axis in biliary NET. Moreover, in the cholangiocarcinoma cell line in which the expression of Notch1 mRNA was knocked down, the mRNA expression of Ascl1 and chromogranin A was increased.ConclusionsThe Notch1-Hes1 signalling axis suppresses neuroendocrine differentiation and maintains tubular/acinar features in adenocarcinoma and non-neoplastic epithelium in the biliary tree. Moreover, a disruption of this signalling axis may be associated with the tumourigenesis of NETs in biliary MANEC.

Neuroendocrine differentiation is not prognostic of failure after radical prostatectomy but correlates with tumor volume

Urology ◽  
2000 ◽  
Vol 56 (6) ◽  
pp. 1011-1015 ◽  
Author(s):  
G Ahlegren ◽  
K Pedersen ◽  
S Lundberg ◽  
G Aus ◽  
J Hugosson ◽  
...  
Keyword(s):  
Radical Prostatectomy ◽  
Tumor Volume ◽  
Neuroendocrine Differentiation

scholarly journals Transcriptomic analysis of mRNA expression and alternative splicing during mouse sex determination

2018 ◽  
Vol 478 ◽  
pp. 84-96 ◽  
Author(s):  
Liang Zhao ◽  
Chenwei Wang ◽  
Melanie L. Lehman ◽  
Mingyu He ◽  
Jiyuan An ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Sex Determination ◽  
Mrna Expression ◽  
Transcriptomic Analysis

scholarly journals Focal Neuroendocrine Differentiation of Conventional Prostate Adenocarcinoma as a Prognostic Factor after Radical Prostatectomy: A Systematic Review and Meta-Analysis

2019 ◽  
Vol 20 (6) ◽  
pp. 1374 ◽  
Author(s):  
Mehdi Kardoust Parizi ◽  
Takehiro Iwata ◽  
Shoji Kimura ◽  
Florian Janisch ◽  
Mohammad Abufaraj ◽  
...  
Keyword(s):  
Systematic Review ◽  
Radical Prostatectomy ◽  
Meta Analysis ◽  
Neuroendocrine Differentiation ◽  
Progression Free Survival ◽  
Fixed Effect Model ◽  
Prostate Adenocarcinoma ◽  
Cochrane Library ◽  
Effect Model ◽  
The Impact

The biologic and prognostic value of focal neuroendocrine differentiation (NED) in conventional prostate adenocarcinoma (PC) patients who undergo radical prostatectomy (RP) remains controversial. In this systematic review and meta-analysis, we assessed the association of focal NED in conventional PC with oncological outcomes after RP. A literature search using PubMed, Scopus, Web of Science, and Cochrane Library was conducted on December 2018 to find relevant studies according to the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. We used a fixed-effect model to analyze the impact of focal NED in RP specimen on progression-free survival defined by biochemical recurrence (BCR). A total of 16 studies with the outcomes of disease progression and survival were eligible. No patient in these studies received androgen deprivation therapy prior to RP. Eleven studies found no significant correlation between focal NED and outcomes of interest, while five studies reported a significant association of focal NED assessed by immunohistochemical chromogranin A or serotonin staining with BCR or survival. Focal NED was associated with higher BCR rates after RP with a pooled HR of 1.39 (95% CI 1.07‒1.81) in five studies. No heterogeneity was reported in this analysis (I2 = 21.7%, p = 0.276). In conclusion, focal NED in conventional PC is associated with worse prognosis after RP. Its presence should be reported in pathologic reports and its true clinical impact should be assessed in well-designed prospective controlled studies.

Higher Tumor to Benign Ratio of the Androgen Receptor mRNA Expression Associates with Prostate Cancer Progression after Radical Prostatectomy

Urology ◽  
2007 ◽  
Vol 70 (6) ◽  
pp. 1225-1229 ◽  
Author(s):  
Inger L. Rosner ◽  
Lakshmi Ravindranath ◽  
Bungo Furusato ◽  
Yongmei Chen ◽  
Chunling Gao ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Radical Prostatectomy ◽  
Mrna Expression ◽  
Cancer Progression ◽  
Prostate Cancer Progression ◽  
Receptor Mrna
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