scholarly journals Determination of the Presence of Diphtheria Toxin in the Myocardial Tissue of Rabbits and a Female Subject by Using an Immunofluorescent Antibody Method

2015 ◽  
Vol 7 (6) ◽  
pp. 472-478
Author(s):  
Mehmet Ceyhan ◽  
Yasemin Ozsurekci ◽  
Merve M. Aydin ◽  
Kamil Can Akcali ◽  
Beril Talim ◽  
...  
Keyword(s):  
Diphtheria Toxin ◽  
Female Subject ◽  
Myocardial Tissue ◽  
Immunofluorescent Antibody ◽  
Antibody Method

Modification of the choriogonadotropin beta-subunit radioimmunoassay for determination of urinary choriogonadotropin.

1978 ◽  
Vol 24 (11) ◽  
pp. 1958-1961 ◽  
Author(s):  
J McCready ◽  
G D Braunstein ◽  
D Helm ◽  
M E Wade
Keyword(s):  
Pregnant Women ◽  
Radioreceptor Assay ◽  
Beta Subunit ◽  
Urine Samples ◽  
Human Choriogonadotropin ◽  
Analytical Recovery ◽  
Antibody Method ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial dilutions of urine containing choriogonadotropin by adding pooled urine from men to the standard tubes and limiting the volume of urine to 100 microliter. This modified assay has a sensitivity of 3 int. units/liter of urine and is specific for choriogonadotropin concentrations of 40 int. units/liter of urine. Analytical recovery of choriogonadotropin added to urine ranged from 96 to 105%. The within-assay CV was 7.6%; the between-assay CV was 11.8%. Concentrations of choriogonadotropin in concurrently collected serum and urine samples from pregnant women correlated well. The test can be performed within 24 h by using the double-antibody method for separating bound from free hormone, or in 3 h with a dioxane method. The assay is about 20-fold more sensitive than the 2-min or 2-h slide and tube pregnancy tests, and seven-to 12-fold more sensitive than the radioreceptor assay.

Micro cell culture method for determination of diphtheria toxin and antitoxin titres using VERO cells

1974 ◽  
Vol 2 (3) ◽  
pp. 203-209 ◽  
Author(s):  
Kikuko Miyamura ◽  
Etsuko Tajiri ◽  
A. Ito ◽  
R. Murata ◽  
R. Kono
Keyword(s):  
Cell Culture ◽  
Diphtheria Toxin ◽  
Culture Method ◽  
Vero Cells

An improved double antibody radioimmunoassay for the determination of insulin in serum, plasma, and tissue incubation media

1982 ◽  
Vol 60 (10) ◽  
pp. 962-966 ◽  
Author(s):  
Marthe Dalpé-Scott ◽  
H. M. C. Heick ◽  
Nicole Bégin-Heick
Keyword(s):  
Experimental Animals ◽  
Limited Size ◽  
Double Antibody ◽  
Incubation Periods ◽  
Free Insulin ◽  
Antibody Method ◽  
Nonspecific Interactions ◽  
Insulin Radioimmunoassay ◽  
Pig Serum

We have modified the double antibody method of insulin radioimmunoassay to allow relatively short incubation periods and the use of centrifugation to separate bound from free insulin. This was achieved by altering the order of addition of reagents and by adding normal guinea-pig serum to reduce nonspecific interactions. The method allows for precise measurements in the range of 0–3.2 ng insulin. Both serum and plasma give consistent values. The technique is useful for the measurement of insulin levels in samples of limited size such as those from small experimental animals.

Quantitative Determination of Immunoglobulins in CSF and Plasma of Chronic Schizophrenic Patients

1981 ◽  
Vol 139 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Lynn E. Delisi ◽  
Daniel R. Weinberger ◽  
Steven Potkin ◽  
Leonard M. Neckers ◽  
David J. Shiling ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Quantitative Determination ◽  
Immune Functioning ◽  
Antibody Technique ◽  
Chronic Schizophrenic ◽  
Immunofluorescent Antibody ◽  
Serum Iga ◽  
Schizophrenic Patients ◽  
Immunoglobulin Levels

SummaryImmunoglobulins, IgG, IgA and IgM were quantified in cerebrospinal fluid (CSF) and plasma from chronic schizophrenic patients and controls using an immunofluorescent antibody technique. A generalized reduction in immunoglobulin levels was observed in the schizophrenic patients compared with controls. While this study supports other reports of abnormal immune functioning in schizophrenia, it failed to replicate previous findings of elevations in CSF IgG and elevations in serum IgA. The aetiology and significance of these findings are hypothesized but remain elusive.

scholarly journals Rapid Enzyme Immunoassay for Determination of Toxigenicity among Clinical Isolates of Corynebacteria

2000 ◽  
Vol 38 (4) ◽  
pp. 1385-1389 ◽  
Author(s):  
Kathryn H. Engler ◽  
Androulla Efstratiou
Keyword(s):  
Enzyme Immunoassay ◽  
Diphtheria Toxin ◽  
Culture Media ◽  
Clinical Isolates ◽  
Diverse Range ◽  
Cell Tissue ◽  
Toxin Protein ◽  
Capture Antibody ◽  
Working Day

A rapid enzyme immunoassay (EIA) was developed for the phenotypic detection of diphtheria toxin among clinical isolates of corynebacteria. The assay uses equine polyclonal antitoxin as the capture antibody and an alkaline phosphatase-labeled monoclonal antibody, specific for fragment A of the toxin molecule, as the detecting antibody. The assay is rapid, sensitive, and specific: a final result is available within 3 h of colony selection, and the limits of detection are 0.1 ng of pure diphtheria toxin/ml. Toxigenicity could be detected with isolates grown on a diverse range of culture media, including selective agars. Toxin detection using the EIA was compared to that with the Elek test and PCR detection of fragment A of the diphtheria toxin (tox) gene, using 245 isolates of corynebacteria. The results for the EIA were in complete concordance with those of the Elek test: 87 toxigenic and 158 nontoxigenic isolates. Ten of the phenotypically nontoxigenic strains were found to contain fragment A of the tox gene but did not express the toxin protein. These isolates were found to be nontoxigenic in the Vero cell tissue culture cytotoxicity assay and were therefore nontoxigenic for diagnostic purposes. The EIA is a simple rapid phenotypic test which provides a definitive result on toxigenicity within one working day.

Development of an HPLC method for determination of metabolic compounds in myocardial tissue

2004 ◽  
Vol 35 (3) ◽  
pp. 647-653 ◽  
Author(s):  
M.G. Volonté ◽  
G. Yuln ◽  
P. Quiroga ◽  
A.E. Consolini
Keyword(s):  
Hplc Method ◽  
Myocardial Tissue
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