scholarly journals Successful Bacterial Engraftment Identified by Next-Generation Sequencing Predicts Success of Fecal Microbiota Transplant for Clostridioides difficile

2021 ◽  
Vol 14 (5) ◽  
pp. 304-309
Author(s):  
Sabine Hazan ◽  
Sonya Dave ◽  
Andreas J. Papoutsis ◽  
Brad D. Barrows ◽  
Thomas J. Borody
Keyword(s):  
Next Generation Sequencing ◽  
Fecal Microbiota ◽  
Next Generation ◽  
Fecal Microbiota Transplant ◽  
Clostridioides Difficile ◽  
Generation Sequencing

scholarly journals Genomic characterization of fecal microbial communities in severely sick humans and broiler chickens using next generation sequencing

2020 ◽  
Author(s):  
Ome Kalsoom Afridi ◽  
Johar Ali ◽  
Jeong Ho Chang
Keyword(s):  
Next Generation Sequencing ◽  
Microbial Communities ◽  
Broiler Chickens ◽  
Human Subjects ◽  
Fecal Microbiota ◽  
Next Generation ◽  
Microbial Association ◽  
Taxonomic Profiling ◽  
Microbial Dysbiosis ◽  
Generation Sequencing

Abstract Background: Microbiota plays an important role in food safety and its alteration poses a serious threat to humans. Comparative microbiome profiling using next-generation sequencing (NGS) enabled the understanding of microbial diversity and similarity between different species. In this study, we used NGS to profile the fecal microbiota of sick human and broiler chickens. A total of 26 fecal samples were collected from severely sick human subjects (n= 13) and broiler chickens (n=13) with similar symptoms. Results: The total number of microbial species detected in broiler chickens fecal microbiota was higher than that of humans. Phylum Proteobacteria was the most abundant in both human and broiler chickens fecal microbiota while Tenericutes was found to be least abundant in both species. Phylum Actinobacteria was found only in the human fecal microbiota. In both humans and broiler chickens, E.coli was found to be phylogenetically related suggesting a microbial association between both species. Conclusion: NGS based taxonomic profiling revealed the association of microbial dysbiosis with extreme sickness in both humans and broiler chickens. The dominance of phylum Proteobacteria in both the species ascertains their altered gut microbiota. Both human and broiler chickens microbial communities were found to be genetically related indicating horizontal transfer of microbes between the two species.

scholarly journals Genomic characterization of fecal microbial communities in severely sick humans and broiler chickens using next generation sequencing

2020 ◽  
Author(s):  
Ome Kalsoom Afridi ◽  
Johar Ali ◽  
Jeong Ho Chang
Keyword(s):  
Next Generation Sequencing ◽  
Microbial Communities ◽  
Broiler Chickens ◽  
Human Subjects ◽  
Fecal Microbiota ◽  
Next Generation ◽  
Microbial Association ◽  
Taxonomic Profiling ◽  
Microbial Dysbiosis ◽  
Generation Sequencing

Abstract BackgroundMicrobiota plays an important role in food safety and its alteration poses a serious threat to humans. Comparative microbiome profiling using next-generation sequencing (NGS) enabled the understanding of microbial diversity and similarity between different species. In this study, we used NGS to profile the fecal microbiota of sick human and broiler chickens. A total of 26 fecal samples were collected from severely sick human subjects (n= 13) and broiler chickens (n=13) with similar symptoms.ResultsThe total number of microbial species detected in broiler chickens fecal microbiota was higher than that of humans. Phylum Proteobacteria was the most abundant in both human and broiler chickens fecal microbiota while Tenericutes was found to be least abundant in both species. Phylum Actinobacteria was found only in the human fecal microbiota. In both humans and broiler chickens, E.coli was found to be phylogenetically related suggesting a microbial association between both species.ConclusionNGS based taxonomic profiling revealed the association of microbial dysbiosis with extreme sickness in both humans and broiler chickens. The dominance of phylum Proteobacteria in both the species ascertains their altered gut microbiota. Both human and broiler chickens microbial communities were found to be genetically related indicating horizontal transfer of microbes between the two species.

Labordiagnostik bei gastrointestinalen Tumoren

2020 ◽  
Vol 11 (05) ◽  
pp. 232-238
Author(s):  
Marcus Kleber
Keyword(s):  
Next Generation Sequencing ◽  
Kolorektales Karzinom ◽  
Next Generation ◽  
Generation Sequencing

ZUSAMMENFASSUNGDas kolorektale Karzinom (KRK) ist einer der häufigsten malignen Tumoren in Deutschland. Einer frühzeitigen Diagnostik kommt große Bedeutung zu. Goldstandard ist hier die Koloskopie. Die aktuelle S3-Leitlinie Kolorektales Karzinom empfiehlt zum KRK-Screening den fäkalen okkulten Bluttest. Für das Monitoring von Patienten vor und nach Tumorresektion werden die Messung des Carcinoembryonalen Antigens (CEA) und der Mikrosatellitenstabilität empfohlen. Für die Auswahl der korrekten Chemotherapie scheint derzeit eine Überprüfung des Mutationsstatus, mindestens des KRAS-Gens und des BRAF-Gens, sinnvoll zu sein. Eine Reihe an neuartigen Tumormarkern befindet sich momentan in der Entwicklung, hat jedoch noch nicht die Reife für eine mögliche Anwendung in der Routinediagnostik erreicht. Den schnellsten Weg in die breite Anwendung können Next-Generation-Sequencing-basierte genetische Tests finden.

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