The Anti-apoptotic Mechanism of Metformin Against Apoptosis Induced by Ionizing Radiation in Human Peripheral Blood Mononuclear Cells

2017 ◽  
Vol 30 (5) ◽  
pp. 372-379 ◽  
Author(s):  
Sedighe Kolivand ◽  
Elahe Motevaseli ◽  
Mohsen Cheki ◽  
Aziz Mahmoudzadeh ◽  
Alireza Shirazi ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Apoptotic Mechanism

scholarly journals Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells

2016 ◽  
Vol 58 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Lucian Beer ◽  
Lucas Nemec ◽  
Tanja Wagner ◽  
Robin Ristl ◽  
Lukas M. Altenburger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Ionizing Radiation ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Non Coding Rnas

AbstractLong non-coding RNAs (lncRNAs) are non-protein coding transcripts that modulate mRNA and microRNA (miRNA) expression, thereby controlling multiple cellular processes, including transcriptional regulation of gene expression, cell differentiation and apoptosis. Ionizing radiation (IR), a strong cellular stressor, is known to influence gene expression of irradiated cells, mainly by activation of oxidative processes. Whether and how IR also affects lncRNA expression in human peripheral blood mononuclear cells (PBMCs) is still poorly understood. Exposure of PBMCs to IR dose-dependently activated p53 and its downstream target p21, ultimately leading to cell-cycle arrest and/or apoptosis. Cleavage of caspase-3, a specific process during apoptotic cell death, was detectable at doses as low as 30 Gy. Transcriptome analysis of 60 Gy–irradiated PBMCs revealed a strong time-dependent regulation of a variety of lncRNAs. Among many unknown lncRNAs we also identified a significant upregulation of Trp53cor1, MEG3 and TUG1, which have been shown to be involved in the regulation of cell cycle and apoptotic processes mediated by p53. In addition, we found 177 miRNAs regulated in the same samples, including several miRNAs that are known targets of upregulated lncRNAs. Our data show that IR dose-dependently regulates the expression of a wide spectrum of lncRNAs in PBMCs, suggesting a crucial role for lncRNAs in the complex regulatory machinery activated in response to IR.

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