Spatial and temporal regulation of three collagen genes during sensory ray morphogenesis of caenorhabditis elegans

2006 ◽  
Author(s):  
Wing-sze Hui
Keyword(s):  
Caenorhabditis Elegans ◽  
Temporal Regulation ◽  
Sensory Ray ◽  
Collagen Genes

Hox gene expression in a single Caenorhabditis elegans cell is regulated by a caudal homolog and intercellular signals that inhibit wnt signaling

Development ◽  
1999 ◽  
Vol 126 (4) ◽  
pp. 805-814 ◽  
Author(s):  
C.P. Hunter ◽  
J.M. Harris ◽  
J.N. Maloof ◽  
C. Kenyon
Keyword(s):  
Caenorhabditis Elegans ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Postembryonic Development ◽  
Regulatory Pathways ◽  
Hox Gene ◽  
Sensory Ray ◽  
Body Regions ◽  
Anterior Body

In Caenorhabditis elegans males, a row of epidermal precursor cells called seam cells generates a pattern of cuticular alae in anterior body regions and neural sensilla called rays in the posterior. The Hox gene mab-5 is required for two posterior seam cells, V5 and V6, to generate rays. In mab-5 mutant males, V5 and V6 do not generate sensory ray lineages but instead generate lineages that lead to alae. Here we show that two independent regulatory pathways can activate mab-5 expression in the V cells. First, the caudal homolog pal-1 turns on mab-5 in V6 during embryogenesis. Second, a Wnt signaling pathway is capable of activating mab-5 in the V cells during postembryonic development; however, during normal development Wnt signaling is inhibited by signals from neighboring V cells. The inhibition of this Wnt signaling pathway by lateral signals between the V cells limits the number of rays in the animal and also determines the position of the boundary between alae and rays.

Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans

1985 ◽  
Vol 5 (2) ◽  
pp. 363-372
Author(s):  
G N Cox ◽  
D Hirsh
Keyword(s):  
Caenorhabditis Elegans ◽  
Protein Composition ◽  
Large Family ◽  
Major Protein ◽  
Mrna Levels ◽  
Mrna Accumulation ◽  
C Elegans ◽  
Collagen Protein ◽  
Protein Components ◽  
Collagen Genes

Collagens are the major protein components of the Caenorhabditis elegans cuticle and are encoded by a large family of 40 to 150 closely related but nonidentical genes. We have determined temporal patterns of mRNA accumulation for a large number of collagen genes by screening recombinant phages and plasmids containing cloned collagen genes under high stringency conditions with 32P-labeled cDNA preparations specific for eggs or three postembryonic molts. We find that collagen mRNA levels are regulated both temporally and quantitatively during C. elegans development. Most genes studied exhibit one of four patterns of mRNA accumulation which correlate with changes in cuticle morphology and collagen protein composition during development. Our results suggest that, in general, there is a progressive activation of new collagen genes during normal development.

scholarly journals Selective lineage specification by mab-19 during Caenorhabditis elegans male peripheral sense organ development.

Genetics ◽  
1994 ◽  
Vol 138 (3) ◽  
pp. 675-688 ◽  
Author(s):  
M E Sutherlin ◽  
S W Emmons
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Physiological Stress ◽  
Developmental Regulation ◽  
Cell Lineage ◽  
Sense Organ ◽  
Sensory Ray ◽  
Hypodermal Cell ◽  
Physiological Stress Response ◽  
Male Specific

Abstract The action of the gene mab-19 is required for specification of a subset of Caenorhabditis elegans male peripheral sense organ (ray) lineages. Two mab-19 alleles, isolated in screens for ray developmental mutations, resulted in males that lacked the three most posterior rays. Cell lineage alterations of male-specific divisions of the most posterior lateral hypodermal (seam) blast cell, T, resulted in the ray loss phenotype in mab-19 mutant animals. Postembryonic seam lineage defects were limited to male-specific T descendent cell divisions. Embryonic lethality resulted when either mab-19 mutation was placed over a chromosomal deficiency encompassing the mab-19 locus. The earliest detectable defect was aberrant hypodermal cell movements during morphogenesis. From these data, it is inferred that both mab-19 alleles described are hypomorphs, and further reduction of mab-19 function results in embryos that are unable to complete morphogenesis. Thus, mab-19 may play a larger role in developmental regulation of hypodermal cell fate, including sensory ray development in males. Body morphology mutations, passage through the dauer stage, and heat or CdCl2 treatment suppressed mab-19 male phenotypes. A model is presented in which all three types of suppression result in a physiological stress response, which in turn leads to correction of the mab-19 defect.

Comparisons of the complete sequences of two collagen genes from Caenorhabditis elegans

Cell ◽  
1982 ◽  
Vol 30 (2) ◽  
pp. 599-606 ◽  
Author(s):  
James M. Kramer ◽  
George N. Cox ◽  
David Hirsh
Keyword(s):  
Caenorhabditis Elegans ◽  
Complete Sequences ◽  
Collagen Genes

scholarly journals Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing

2016 ◽  
Vol 6 (12) ◽  
pp. 4077-4086 ◽  
Author(s):  
Theresa L B Edelman ◽  
Katherine A McCulloch ◽  
Angela Barr ◽  
Christian Frøkjær-Jensen ◽  
Erik M Jorgensen ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Null Allele ◽  
Postembryonic Development ◽  
Developmental Timing ◽  
Critical Element ◽  
Coding Region ◽  
Protein Coding ◽  
Temporal Regulation ◽  
Larval Stages ◽  
Hypomorphic Alleles

Abstract The Caenorhabditis elegans heterochronic gene pathway regulates the relative timing of events during postembryonic development. lin-42, the worm homolog of the circadian clock gene, period, is a critical element of this pathway. lin-42 function has been defined by a set of hypomorphic alleles that cause precocious phenotypes, in which later developmental events, such as the terminal differentiation of hypodermal cells, occur too early. A subset of alleles also reveals a significant role for lin-42 in molting; larval stages are lengthened and ecdysis often fails in these mutant animals. lin-42 is a complex locus, encoding overlapping and nonoverlapping isoforms. Although existing alleles that affect subsets of isoforms have illuminated important and distinct roles for this gene in developmental timing, molting, and the decision to enter the alternative dauer state, it is essential to have a null allele to understand all of the roles of lin-42 and its individual isoforms. To remedy this problem and discover the null phenotype, we engineered an allele that deletes the entire lin-42 protein-coding region. lin-42 null mutants are homozygously viable, but have more severe phenotypes than observed in previously characterized hypomorphic alleles. We also provide additional evidence for this conclusion by using the null allele as a base for reintroducing different isoforms, showing that each isoform can provide heterochronic and molting pathway activities. Transcript levels of the nonoverlapping isoforms appear to be under coordinate temporal regulation, despite being driven by independent promoters. The lin-42 null allele will continue to be an important tool for dissecting the functions of lin-42 in molting and developmental timing.

scholarly journals Number and organization of collagen genes in Caenorhabditis elegans.

1984 ◽  
Vol 4 (11) ◽  
pp. 2389-2395 ◽  
Author(s):  
G N Cox ◽  
J M Kramer ◽  
D Hirsh
Keyword(s):  
Caenorhabditis Elegans ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Phage Library ◽  
Collagen Gene ◽  
Recombinant Phage ◽  
C Elegans ◽  
Dna Libraries ◽  
Collagen Genes

We analyzed the number and organization of collagen genes in the nematode Caenorhabditis elegans. Genomic Southern blot hybridization experiments and recombinant phage library screenings indicated that C. elegans has between 40 and 150 distinct collagen genes. A large number of recombinant phages containing collagen genes were isolated from C. elegans DNA libraries. Physical mapping studies indicated that most phage contained a single small collagen gene less than 3 kilobases in size. A few phage contained multiple collagen hybridizing regions and may contain a larger collagen gene or several tightly linked small collagen genes. No overlaps were observed between phages containing different collagen genes, implying that the genes are dispersed in the C. elegans genome. Consistent with the small size of most collagen genes, we found that the predominant class of collagen mRNA in C. elegans is 1.2 to 1.4 kilobases in length. Genomic Southern blot experiments under stringent hybridization conditions revealed considerable sequence diversity among collagen genes. Our data suggest that most collagen genes are unique or are present in only a few copies.

Temporal regulation of cuticle synthesis during development of Caenorhabditis elegans

1981 ◽  
Vol 84 (2) ◽  
pp. 277-285 ◽  
Author(s):  
George N. Cox ◽  
Meredith Kusch ◽  
Karin DeNevi ◽  
Robert S. Edgar
Keyword(s):  
Caenorhabditis Elegans ◽  
Temporal Regulation

scholarly journals An extracellular matrix protein promotes anillin-dependent processes in the Caenorhabditis elegans germline

2019 ◽  
Vol 2 (2) ◽  
pp. e201800152
Author(s):  
Hongxia Lan ◽  
Xinyan Wang ◽  
Ling Jiang ◽  
Jianjian Wu ◽  
Xuan Wan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Caenorhabditis Elegans ◽  
Matrix Protein ◽  
Genetic Material ◽  
Extracellular Matrix Protein ◽  
Contractile Ring ◽  
Division Plane ◽  
Temporal Regulation ◽  
Intracellular Signals ◽  
Extracellular Factor

Cell division requires constriction of an actomyosin ring to segregate the genetic material equally into two daughter cells. The spatial and temporal regulation of the contractile ring at the division plane primarily depends on intracellular signals mediated by the centralspindlin complex and astral microtubules. Although much investigative work has elucidated intracellular factors and mechanisms controlling this process, the extracellular regulation of cytokinesis remains unclear. Thus far, the extracellular matrix protein Hemicentin (HIM-4) has been proposed to be required for cleavage furrow stabilization. The underlying molecular mechanism, however, has remained largely unknown. Here, we show that HIM-4 and anillin (ANI-1) genetically act in the same pathway to maintain the rachis bridge stability in the germline. Our FRAP experiments further reveal that HIM-4 restricts the motility of ANI-1. In addition, we demonstrate that HIM-4 is recruited to the cleavage site in dividing germ cells and promotes the proper ingression of the cleavage membrane. Collectively, we propose that HIM-4 is an extracellular factor that regulates ANI-1 for germ cell membrane stabilization and contractile ring formation in Caenorhabditis elegans germline cells.

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