Identification and characterization of genes associated with mutant P53 3V 3A 3L¹³u-induced cell transformation in Rat 6 fibroblast cell line

1999 ◽  
Author(s):  
Judy Wai Ping Yam
Keyword(s):  
Cell Line ◽  
Cell Transformation ◽  
Fibroblast Cell ◽  
Mutant P53 ◽  
Fibroblast Cell Line ◽  
Identification And Characterization

scholarly journals Modeling atrial fibrosis in vitro —Generation and characterization of a novel human atrial fibroblast cell line

FEBS Open Bio ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1210-1218 ◽  
Author(s):  
Stephan R. Künzel ◽  
Johanna S. E. Rausch ◽  
Charlotte Schäffer ◽  
Maximilian Hoffmann ◽  
Karolina Künzel ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Atrial Fibrosis

Establishment and characterization of a fibroblast cell line from the Mongolian horse

2009 ◽  
Vol 45 (7) ◽  
pp. 311-316 ◽  
Author(s):  
Lin-feng Li ◽  
Wei-jun Guan ◽  
Yue Hua ◽  
Xiu-juan Bai ◽  
Yue-hui Ma
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Mongolian Horse

scholarly journals Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0195128 ◽  
Author(s):  
Sun Yajing ◽  
Imran Rashid Rajput ◽  
Huang Ying ◽  
Yu Fei ◽  
Edmond Sanganyado ◽  
...  
Keyword(s):  
Cell Line ◽  
Killer Whale ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line

Morphologic, Immunophenotypic and Bioenergetic Characterization of a Human Dermal Fibroblast Cell Line

2014 ◽  
Author(s):  
Ioana Zinuca Pavel ◽  
Oana Duicu ◽  
Florina Bojin ◽  
Andreea Privistirescu ◽  
Dorina Coricovac ◽  
...  
Keyword(s):  
Cell Line ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cell Line

scholarly journals Morphologic, Immunophenotypic and Bioenergetic Characterization of a Human Dermal Fibroblast Cell Line

2014 ◽  
Author(s):  
Ioana Zinuca Pavel ◽  
Oana Duicu ◽  
Florina Bojin ◽  
Andreea Privistirescu ◽  
Dorina E Coricovac ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Line ◽  
Mitochondrial Function ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Seeding Density ◽  
Glycolytic Flux ◽  
Fibroblast Cell Line

Background In the past years, ultraviolet-B (UVB)-induced morphological changes in cultured fibroblasts have been systematically studied in relation with photoprotective strategies. The present study was aimed at providing a comprehensive characterization of a human dermal fibroblast cell line, HDFa, in terms of: (i) morphological and phenotypical aspects, and (ii) mitochondrial function and bioenergetics. Methods Morphology of HDFa line was assessed by light microscopy and flow cytometry was used for the evaluation of the the following surface markers: CD34, CD45, CD90, CD73, CD44, CD29, CD117, CD105, CD109, CD26, IL-10R, HLA-DR and TGF-βRIII. Cell monolayers were fixed and stained for CD105, CD117, Ki67, Vimentin, α-smooth muscle actin, Caldesmon, Cytokeratin, and smooth muscle myosin by immunofluorescence. Mitochondrial bioenergetics was measured by using Seahorse Bioscience XF24 extracellular flux analyzer. Different number of cells were seeded in order to obtain the optimal seeding density needed to identify a measurable OCR (oxygen consumption rate) and ECAR (extracellular acidification rate). The following protocol for mitochondrial function assay was tested at 37°C: Basal respiration followed by sequential injections of Oligomycin, FCCP, and Antimycin A (BOFA). Parameters of mitochondrial respiratory function (basal OCR, maximal respiratory capacity, ATP production, and proton leak) together with those of glycolysis (glycolytic flux, glycolytic capacity, and glycolytic reserve), respectively were measured. After each BOFA assay, cells were lysed to quantify total protein by Bradford assay. Results HDFa cells showed elongated flattened form, were adherent and rapidly expanded in culture. HDFa cells were positive for CD90, CD73, CD26, and CD29 at flowcytometry, whereas imunofluorescence microscopy revealed that the cell line was positive for CD105, Ki67, and Vimentin. The optimal mitochondrial and glycolytic flux parameters were reported at 20.000 cells/well, resulting that this seeding density will be used in our further experiments. Conclusions Characterization of HDFa cell line was performed in order to further report on their changes in response to different protocols of UVB exposure in the presence vs. the absence of protective phytochemicals. Research supported by the doctoral fellowship POSDRU/159 /1.5/S/ 136893.

Sign in / Sign up

Export Citation Format

Share Document