Utilization and characterization of photosynthetic bacteria rhodobacter sphaeroides for bioelectricity generation

2015 ◽  
Author(s):  
Man Tung Wong
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Bioelectricity Generation

scholarly journals CHARACTERIZATION OF DIMETHYLSULFOXIDE REDUCTASE, A NOVEL MOLYBDO-ENZYME FROM PHOTOSYNTHETIC BACTERIA, Rhodobacter sphaeroides f.s. denitrificans

1991 ◽  
Vol 7 (Supple) ◽  
pp. 821-824
Author(s):  
SUNAO YAMAZAKI ◽  
NAOYUKI KOBAYASHI ◽  
AKIRA OKUBO ◽  
TOSHIO SATO ◽  
YUZURU TOZAWA ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Dimethylsulfoxide Reductase

scholarly journals Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaomin Wu ◽  
Guang Ma ◽  
Chuanyang Liu ◽  
Xin-yuan Qiu ◽  
Lu Min ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Heterologous Expression ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Fine Chemicals ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Geranyl Diphosphate ◽  
Ribosome Binding ◽  
Geranyl Diphosphate Synthase

Abstract Background Pinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Purple non-sulfur photosynthetic bacteria belong to photosynthetic chassis that are widely used to synthesize natural chemicals. To date, researches on the synthesis of pinene by purple non-sulfur photosynthetic bacteria has not been reported, leaving the potential of purple non-sulfur photosynthetic bacteria synthesizing pinene unexplored. Results Rhodobacter sphaeroides strain was applied as a model and engineered to express the fusion protein of heterologous geranyl diphosphate synthase (GPPS) and pinene synthase (PS), hence achieving pinene production. The reaction condition of pinene production was optimized and 97.51 μg/L of pinene was yielded. Then, genes of 1-deoxy-d-xylulose 5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase and isopentenyl diphosphate isomerase were overexpressed, and the ribosome binding site of GPPS-PS mRNA was optimized, improving pinene titer to 539.84 μg/L. Conclusions In this paper, through heterologous expression of GPPS-PS, pinene was successfully produced in R. sphaeroides, and pinene production was greatly improved by optimizing the expression of key enzymes. This is the first report on pinene produce by purple non-sulfur photosynthetic bacteria, which expands the availability of photosynthetic chassis for pinene production.

Isolation and characterization of a virulent phage for Rhodobacter sphaeroides

1988 ◽  
Vol 149 (5) ◽  
pp. 476-479 ◽  
Author(s):  
Michael Duchrow ◽  
Stefan Heitefuss ◽  
Jutta Kalkus ◽  
Michael Hoppert ◽  
Friedrich Giffhorn
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Virulent Phage ◽  
Isolation And Characterization

Preparative isolation and characterization of the B875 complex from Rhodobacter sphaeroides 2.4.1

1988 ◽  
Vol 66 (5) ◽  
pp. 442-448 ◽  
Author(s):  
Rafael Picorel ◽  
Gabriel Gingras
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Phosphatidyl Ethanolamine ◽  
Sodium Dodecyl ◽  
Pigment Analysis ◽  
Preparative Isolation ◽  
Ethanolamine Phosphatidyl ◽  
Isolation And Characterization ◽  
Detergent System ◽  
Triton X

We have developed a simple and efficient method, using a mixed detergent system of sodium dodecyl sulfate and Triton X-100, for the preparative isolation of theB875 complex from Rhodobacter sphaeroides 2.4.1. As a bonus, the method allows the preparation of both the B875 and B800-850 complexes from the same batch of chromatophores. The preparations are spectrally pure, as indicated by absorption and circular dichroism spectroscopy. The latter method suggests that the Qy band of the B875 complex is due to weakly interacting bacteriochlorophyll molecules. Protein and pigment analysis shows that the B875 complex contains 2 mol of bacteriochlorophyll and 2 mol of sphaeroidene per mol of apoprotein (12 266 g), whereas the B800-850 complex contains 3 mol of bacteriochlorophyll and 1 mol of sphaeroidene per mol of apoprotein (11 497 g). While these stoichiometries are in accord with currently accepted models, they disagree with their published experimental basis. Phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol were found to be present in the B875 complex.

Cloning and characterization of the 5-aminolevulinate synthase gene(s) from Rhodobacter sphaeroides

Gene ◽  
1988 ◽  
Vol 70 (1) ◽  
pp. 139-151 ◽  
Author(s):  
Tai Tsu-Ning ◽  
Mark D. Moore ◽  
Samuel Kaplan
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Aminolevulinate Synthase

Characterization of a Novel Light-Harvesting Mutant of Rhodobacter sphaeroides with Relation to Photohydrogen Production

BioHydrogen ◽  
2007 ◽  
pp. 123-131
Author(s):  
Lyudmila Vasilyeva ◽  
Masato Miyake ◽  
Masayuki Hara ◽  
Eiju Nakada ◽  
Satoshi Nishikata ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Light Harvesting
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