Identification of novel anticancer drug candidates from Chinese medicinal herbs with DNA replication-initiation proteins as the targets

2008 ◽  
Author(s):  
Yi Shen
Keyword(s):  
Dna Replication ◽  
Anticancer Drug ◽  
Medicinal Herbs ◽  
Replication Initiation ◽  
Chinese Medicinal Herbs ◽  
Dna Replication Initiation ◽  
Drug Candidates ◽  
Replication Initiation Proteins

scholarly journals Archaeal Orc1 protein interacts with T-rich single-stranded DNA

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Katarzyna Wegrzyn ◽  
Igor Konieczny
Keyword(s):  
Dna Replication ◽  
Replication Initiation ◽  
Single Stranded Dna ◽  
Dna Replication Initiation ◽  
Double Stranded Dna ◽  
Nucleoprotein Complexes ◽  
Eukaryotic Organisms ◽  
Replication Initiation Proteins ◽  
Hydrolysis Of ◽  
Replication Activity

Abstract Objective The ability to form nucleoprotein complexes is a fundamental activity of DNA replication initiation proteins. They bind within or nearby the region of replication origin what results in melting of a double-stranded DNA (dsDNA) and formation of single-stranded DNA (ssDNA) region where the replication machinery can assemble. For prokaryotic initiators it was shown that they interact with the formed ssDNA and that this interaction is required for the replication activity. The ability to interact with ssDNA was also shown for Saccharomyces cerevisiae replication initiation protein complex ORC. For Archaea, which combine features of both prokaryotic and eukaryotic organisms, there was no evidence whether DNA replication initiators can interact with ssDNA. We address this issue in this study. Results Using purified Orc1 protein from Aeropyrum pernix (ApOrc1) we analyzed its ability to interact with ssDNA containing sequence of an AT-rich region of the A. pernix origin Ori1 as well as with homopolymers of thymidine (polyT) and adenosine (polyA). The Bio-layer interferometry, surface plasmon resonance and microscale thermophoresis showed that the ApOrc1 can interact with ssDNA and it binds preferentially to T-rich ssDNA. The hydrolysis of ATP is not required for this interaction.

scholarly journals The interaction networks of the budding yeast and human DNA replication-initiation proteins

Cell Cycle ◽  
2019 ◽  
Vol 18 (6-7) ◽  
pp. 723-741 ◽  
Author(s):  
Rentian Wu ◽  
Aftab Amin ◽  
Ziyi Wang ◽  
Yining Huang ◽  
Marco Man-Hei Cheung ◽  
...  
Keyword(s):  
Dna Replication ◽  
Budding Yeast ◽  
Replication Initiation ◽  
Interaction Networks ◽  
Dna Replication Initiation ◽  
Human Dna ◽  
Replication Initiation Proteins

scholarly journals Nuclear Organization of DNA Replication Initiation Proteins in Mammalian Cells

2002 ◽  
Vol 277 (12) ◽  
pp. 10354-10361 ◽  
Author(s):  
Masatoshi Fujita ◽  
Yukio Ishimi ◽  
Hiromu Nakamura ◽  
Tohru Kiyono ◽  
Tatsuya Tsurumi
Keyword(s):  
Dna Replication ◽  
Mammalian Cells ◽  
Nuclear Organization ◽  
Replication Initiation ◽  
Dna Replication Initiation ◽  
Replication Initiation Proteins
Sign in / Sign up

Export Citation Format

Share Document