Functional studies of the interaction between human MCM6 and Cdt1 & Characterization of Nog1p for its novel function in DNA replication initiation in budding yeast

2008 ◽  
Author(s):  
Lan Yu
Keyword(s):  
Dna Replication ◽  
Budding Yeast ◽  
Replication Initiation ◽  
Dna Replication Initiation ◽  
Functional Studies

scholarly journals The interaction networks of the budding yeast and human DNA replication-initiation proteins

Cell Cycle ◽  
2019 ◽  
Vol 18 (6-7) ◽  
pp. 723-741 ◽  
Author(s):  
Rentian Wu ◽  
Aftab Amin ◽  
Ziyi Wang ◽  
Yining Huang ◽  
Marco Man-Hei Cheung ◽  
...  
Keyword(s):  
Dna Replication ◽  
Budding Yeast ◽  
Replication Initiation ◽  
Interaction Networks ◽  
Dna Replication Initiation ◽  
Human Dna ◽  
Replication Initiation Proteins

Positive and negative control of helicase recruitment at a bacterial chromosome origin

2021 ◽  
Author(s):  
Charles Winterhalter ◽  
Daniel Stevens ◽  
Stepan Fenyk ◽  
Simone Pelliciari ◽  
Elie Marchand ◽  
...  
Keyword(s):  
Dna Replication ◽  
Model Organism ◽  
Negative Control ◽  
Replication Initiation ◽  
Bacterial Dna ◽  
Dna Replication Initiation ◽  
Recognition Element ◽  
Helicase Loading ◽  
Protein Interfaces

The mechanisms responsible for helicase loading during the initiation of chromosome replication in bacteria are unclear. Here we report both a positive and a negative mechanism for directing helicase recruitment in the model organism Bacillus subtilis. Systematic mutagenesis of the essential replication initiation gene dnaD and characterization of DnaD variants revealed protein interfaces required for interacting with the master initiator DnaA and with a specific single-stranded DNA (ssDNA) sequence located in the chromosome origin (DnaD Recognition Element, DRE). We propose that the location of the DRE within the replication origin orchestrates recruitment of helicase to achieve bidirectional DNA replication. We also report that the developmentally expressed repressor of DNA replication initiation, SirA, acts by blocking the interaction of DnaD with DnaA, thereby inhibiting helicase recruitment to the origin. These findings significantly advance our mechanistic understanding of helicase recruitment and regulation during bacterial DNA replication initiation. Because DnaD is essential for the viability of clinically relevant Gram-positive pathogens, DnaD is an attractive target for drug development.

Characterization of DNA synthesis and chloroplast DNA replication initiation in a Petunia hybrida chloroplast lysate system

1987 ◽  
Vol 12 (5) ◽  
pp. 377-386 ◽  
Author(s):  
Jan M. de Haas ◽  
Ad J. Kool ◽  
Nico Overbeeke ◽  
Wieger van Brug ◽  
H. John ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Chloroplast Dna ◽  
Petunia Hybrida ◽  
Replication Initiation ◽  
Dna Replication Initiation ◽  
Chloroplast Dna Replication

scholarly journals DNA replication initiation in Bacillus subtilis: structural and functional characterization of the essential DnaA–DnaD interaction

2018 ◽  
Vol 47 (4) ◽  
pp. 2101-2112 ◽  
Author(s):  
Eleyna Martin ◽  
Huw E L Williams ◽  
Matthaios Pitoulias ◽  
Daniel Stevens ◽  
Charles Winterhalter ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Dna Replication ◽  
Functional Characterization ◽  
Replication Initiation ◽  
Dna Replication Initiation
Sign in / Sign up

Export Citation Format

Share Document