scholarly journals Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

ALTEX ◽  
2011 ◽  
Vol 28 (4) ◽  
pp. 305-316 ◽  
Author(s):  
Caroline Rauch
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
Fetal Bovine ◽  
Serum Substitute

scholarly journals A plea to reduce or replace fetal bovine serum in cell culture media

2013 ◽  
Vol 65 (5) ◽  
pp. 791-793 ◽  
Author(s):  
Gerhard Gstraunthaler ◽  
Toni Lindl ◽  
Jan van der Valk
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
Fetal Bovine

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

2010 ◽  
Vol 24 (4) ◽  
pp. 1053-1063 ◽  
Author(s):  
J. van der Valk ◽  
D. Brunner ◽  
K. De Smet ◽  
Å. Fex Svenningsen ◽  
P. Honegger ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
In Vitro Methods ◽  
Fetal Bovine

Fetal bovine serum—a cell culture dilemma

Science ◽  
2022 ◽  
Vol 375 (6577) ◽  
pp. 143-144
Author(s):  
Jan van der Valk
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
A Cell ◽  
Fetal Bovine

Ethical and possible reproducibility issues arise when using fetal bovine serum in cell culture media

Human Platelet Lysates Successfully Replace Fetal Bovine Serum in Adipose-Derived Adult Stem Cell Culture

2014 ◽  
Vol 2 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Gerhard Gstraunthaler ◽  
◽  
Caroline Rauch ◽  
Jaqueline Wechselberger ◽  
Elisabeth Feifel
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Adult Stem Cell ◽  
Stem Cell Culture ◽  
Fetal Bovine

scholarly journals Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture

2019 ◽  
Vol 47 (4) ◽  
pp. 487-497
Author(s):  
You Young Jo ◽  
HaeYong Kweon ◽  
Sang Deok Ji ◽  
Jong Gil Kim ◽  
Kee Young Kim
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Silk Protein ◽  
Fetal Bovine ◽  
Serum Substitute

scholarly journals Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions

2019 ◽  
Vol 10 (1) ◽  
pp. 7
Author(s):  
Adi Santoso ◽  
Larasati Larasati ◽  
Arizah Kusumawati ◽  
Popi Hadi Wisnuwardhani ◽  
Ratih Asma Ningrum ◽  
...  
Keyword(s):  
Cell Culture ◽  
Protein Expression ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Culture Conditions ◽  
Expression Level ◽  
Seeding Density ◽  
Cell Culture Media ◽  
Human Erythropoietin ◽  
Fetal Bovine

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation

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