scholarly journals Extending the applicability domain of the human cell line activation test (h-CLAT)

ALTEX ◽  
2020 ◽  
Author(s):  
Karsten Mewes
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Applicability Domain ◽  
Activation Test

Preliminary discovery of novel markers for human cell line activation test (h-CLAT)

2021 ◽  
pp. 105154
Author(s):  
Aneesh V. Karkhanis ◽  
Eric Chun Yong Chan ◽  
Ee Chee Ren
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Activation Test

The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test – human cell line activation test (h-CLAT)

2008 ◽  
Vol 25 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Hitoshi Sakaguchi ◽  
Takao Ashikaga ◽  
Masaaki Miyazawa ◽  
Nanae Kosaka ◽  
Yuichi Ito ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Skin Sensitization ◽  
Activation Test ◽  
The Relationship

Prediction of the skin sensitization potential of didecyldimethylammonium chloride and 3,7-dimethyl-2,6-octadienal and mixtures of these compounds with the excipient ethylene glycol through the human Cell Line Activation Test and the Direct Peptide Reactivity Assay

2019 ◽  
Vol 35 (8) ◽  
pp. 507-519 ◽  
Author(s):  
JaeHee Lee ◽  
AhRang Cho ◽  
Ravi Gautam ◽  
YeonGyeong Kim ◽  
SoJung Shin ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Antimicrobial Agent ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Skin Sensitization ◽  
Active Ingredients ◽  
Activation Test ◽  
Didecyldimethylammonium Chloride

In commercial products such as household deodorants or biocides, didecyldimethylammonium chloride (DDAC) often serves as an antimicrobial agent, citral serves as a fragrance agent, and the excipient ethylene glycol (EG) is used to dissolve the active ingredients. The skin sensitization (SS) potentials of each of these substances are still being debated. Moreover, mixtures of DDAC or citral with EG have not been evaluated for SS potency. The in vitro alternative assay called human Cell Line Activation Test (h-CLAT) and Direct Peptide Reactivity Assay (DPRA) served to address these issues. On three independent runs of h-CLAT, DDAC and citral were predicted to be sensitizers while EG was predicted to be a non-sensitizer and also by the DPRA. Mixtures of DDAC or citral with EG at ratios of 7:3 and 1:4 w/v were all positive by the h-CLAT in terms of SS potential but SS potency was mitigated as the proportion of EG increased. Citral and its EG mixtures were all positive but DDAC and its EG mixtures were all negative by the DPRA, indicating that the DPRA method is not suitable for chemicals with pro-hapten characteristics. Since humans can be occupationally or environmentally exposed to mixtures of excipients with active ingredients, the present study may give insights into further investigations of the SS potentials of various chemical mixtures.

Prediction of skin sensitization potency of chemicals by human Cell Line Activation Test (h-CLAT) and an attempt at classifying skin sensitization potency

2012 ◽  
Vol 26 (7) ◽  
pp. 1150-1160 ◽  
Author(s):  
Yuko Nukada ◽  
Takao Ashikaga ◽  
Masaaki Miyazawa ◽  
Morihiko Hirota ◽  
Hitoshi Sakaguchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Skin Sensitization ◽  
Activation Test

Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT)

2011 ◽  
Vol 65 (6) ◽  
pp. 343-353 ◽  
Author(s):  
Yuko Nukada ◽  
Takao Ashikaga ◽  
Hitoshi Sakaguchi ◽  
Sakiko Sono ◽  
Nanae Mugita ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Human Cell ◽  
Predictive Performance ◽  
Human Cell Line ◽  
Activation Test

scholarly journals Improvement of human cell line activation test (h-CLAT) using short-time exposure methods for prevention of false-negative results

2018 ◽  
Vol 43 (3) ◽  
pp. 229-240 ◽  
Author(s):  
Kazuto Narita ◽  
Yuuki Ishii ◽  
Phuc Thi Hong Vo ◽  
Fumiko Nakagawa ◽  
Shinichi Ogata ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
False Negative ◽  
Human Cell Line ◽  
Activation Test ◽  
Time Exposure ◽  
Negative Results ◽  
False Negative Results ◽  
Short Time ◽  
Exposure Methods
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