scholarly journals Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma

2019 ◽  
Vol 6 (2) ◽  
pp. 112 ◽  
Author(s):  
Praveen K. Bommareddy ◽  
Devin B. Lowe ◽  
Howard L. Kaufman ◽  
Samuel D. Rabkin ◽  
Dipongkor Saha
Keyword(s):  
Flow Cytometry ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immune Cells ◽  
Staining Procedure ◽  
Oncolytic Herpes Simplex Virus ◽  
Flow Cytometry Staining ◽  
Simplex Virus

scholarly journals Oncolytic herpes simplex virus and immunotherapy

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqing Ma ◽  
Hongbin He ◽  
Hongmei Wang
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Oncolytic Herpes Simplex Virus ◽  
Simplex Virus

scholarly journals Enhancing Therapeutic Efficacy of Oncolytic Herpes Simplex Virus-1 with Integrin β1 Blocking Antibody OS2966

2019 ◽  
Vol 18 (6) ◽  
pp. 1127-1136 ◽  
Author(s):  
Tae Jin Lee ◽  
Mitra Nair ◽  
Yeshavanth Banasavadi-Siddegowda ◽  
Joseph Liu ◽  
Tejaswini Nallanagulagari ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Therapeutic Efficacy ◽  
Integrin Β1 ◽  
Herpes Simplex Virus 1 ◽  
Blocking Antibody ◽  
Oncolytic Herpes Simplex Virus ◽  
Simplex Virus

scholarly journals Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet.

1997 ◽  
Vol 41 (12) ◽  
pp. 2686-2692 ◽  
Author(s):  
I Pavić ◽  
A Hartmann ◽  
A Zimmermann ◽  
D Michel ◽  
W Hampl ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cytopathic Effect ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Resistant Strains ◽  
Simplex Virus

We established a quantitative flow cytometric method for determination of herpes simplex virus type 1 (HSV-1) susceptibility to acyclovir (ACV), ganciclovir, and foscarnet in vitro. Susceptibility was defined in terms of the drug concentration which reduced the number of cells expressing HSV-1 glycoprotein C (gpC) with a fluorescence intensity of > or =10(2) by 50% (IC50). Flow cytometry allowed us to use a high (1.0) as well as a low (0.005) multiplicity of infection, and determination of the IC50 was possible after one or more viral replicative cycles. IC50s were dependent on virus input and on time postinfection. In mixture experiments, 1 to 2% resistant viruses added to a sensitive strain could be detected. The results obtained by flow cytometry showed a good qualitative correlation with those achieved by cytopathic effect inhibitory assay. However, flow cytometry might detect more quantitative differences in drug susceptibility, especially among resistant strains, as confirmed also by determination of intracellular drug phosphorylation. The mean IC50s for ACV-sensitive strains were 0.45 to 1.47 microM, and those for ACV-resistant strains were between 140 and 3,134 microM. Flow cytometric analysis was fast and accurate, automatizable, and highly reproducible. Flow cytometry may be a more powerful tool than standard cytopathic effect-based assays and could have advantages for the detection of low levels of drug resistance or mixtures of sensitive and resistant virus strains.

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