scholarly journals Comprehensive analysis of signal transduction in three-dimensional ECM-based tumor cell cultures

2015 ◽  
Vol 2 (4) ◽  
pp. 31 ◽  
Author(s):  
Iris Eke ◽  
Stephanie Hehlgans ◽  
Yaping Zong ◽  
Nils Cordes
Keyword(s):  
Signal Transduction ◽  
Tumor Cell ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Comprehensive Analysis

Cell cultures of human malignant tumors in development of new anticancer therapies

2018 ◽  
pp. 13-23
Author(s):  
А.Н. Чернов ◽  
Е.П. Баранцевич ◽  
Э.С. Галимова ◽  
М.М. Галагудза
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Cell Cultures ◽  
Malignant Tumors ◽  
Three Dimensional ◽  
Human Tumor ◽  
Human Tumor Cell ◽  
Culture Models ◽  
Tumor Cell Culture ◽  
Cell Culture Models

Современный эффективный скрининг новых противоопухолевых химиопрепаратов и биологических препаратов на доклиническом этапе невозможен без применения моделей культур опухолевых клеток. К таким моделям относят первичные культуры клеток и клеточные линии опухолей человека, культивируемые в двумерной (2D) и трехмерной (3D) системах. В обзоре обсуждаются различные аспекты применения моделей клеточных культур неоплазий человека, их актуальность в исследованиях противоопухолевой эффективности препаратов. Current effective preclinical screening of new anticancer chemotherapies and biological medicines requires cancer cell culture models. Such models include primary cell cultures and human tumor cell lines cultured in two-dimensional (2D) and three-dimensional (3D) systems. This review discussed different aspects of using human tumor cell culture models and their relevance for studying efficacy of antitumor drugs.

scholarly journals Three-dimensional tumor cell cultures employed in virotherapy research

2018 ◽  
Vol Volume 7 ◽  
pp. 79-93 ◽  
Author(s):  
Linus Kloker ◽  
Can Yurttas ◽  
Ulrich Lauer
Keyword(s):  
Tumor Cell ◽  
Cell Cultures ◽  
Three Dimensional

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

1968 ◽  
Vol 26 ◽  
pp. 164-165
Author(s):  
R. I. Johnsson-Hegyeli ◽  
A. F. Hegyeli ◽  
D. K. Landstrom ◽  
W. C. Lane
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Cultures ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Three Dimensional ◽  
Malignant Cell ◽  
Interference Microscopy ◽  
Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Autonomous Operation of 3D DNA Walkers in Living Cells for MicroRNA Imaging

Nanoscale ◽  
2021 ◽  
Author(s):  
Hui Hu ◽  
Fu Zhou ◽  
Baojuan Wang ◽  
Xin Chang ◽  
Tianyue Dai ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Three Dimensional ◽  
Living Cells ◽  
Autonomous Operation ◽  
Dna Walkers

Three dimensional (3D) DNA walkers possesses the potential as ideal candidates for signal transduction and amplification in bioassays. However, intracellularly autonomous operation of 3D DNA walkers is still limitedly implemented...

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