scholarly journals Application of Enriched Fraction of Seabuckthorn Leaf Extract as Antimicrobial Finish on Technical Textile

2017 ◽  
Vol 2 (4) ◽  
pp. 428
Author(s):  
M.S. Yogendra Kumar ◽  
T. S. Raghu ◽  
F. V. Varghese ◽  
T. M. Kotresh
Keyword(s):  
High Performance ◽  
Diffusion Method ◽  
Percentage Reduction ◽  
Reduction Test ◽  
Agar Diffusion ◽  
Coated Fabric ◽  
Significant Difference ◽  
Agar Diffusion Method ◽  
Wash Durability ◽  
Triethylene Tetramine

<p class="p1">Flavonoid-rich fraction (FRF) from Seabuckthorn leaves extract was prepared by acid hydrolysis process. Total flavonoid content of Seabuckthorn leaves extract and FRF estimated as rutin equivalent was found to be 116.98±3.06 and 277.14 ± 6.78 mg/g of extract/FRF respectively. Its major constituents myrcetin, quercetin, kaempferol and isorhamnetin, were determined by reverse phase high performance liquid chromatography (RP-HPLC). Aramid (NomexIIIA) fabric was treated with triethylene tetramine to increase the wicking height of the fabric for better uptake of FRF. Then, FRF was coated using citric acid as cross linking agent on to aramid fabric by pad-dry-cure method for improved wash durability. FRF coated fabric was characterised using Universal attenuated total internal reflection Fourier Transform Infrared spectroscopy. Effect of FRF coating on flammability property of coated fabric was estimated using flammability tester. There was no significant difference in the char length of the FRF coated fabric and control samples.<span class="Apple-converted-space"> </span></p><p class="p2">Antimicrobial activity of the FRF coated fabric was assessed by both qualitative (agar diffusion method; AATCC 147-2001) and quantitative (percentage reduction test; (AATCC 100-2001) methods using test organisms. The zone of inhibition by agar diffusion method for <em>E. coli </em>and <em>S. aureus </em>was found to be 12.4 mm and 16.7 mm respectively. Quantitative assessment by percentage reduction test showed a reduction percentage of 96.00% and 93.00% for <em>S. aureus </em>and <em>E</em>. <em>coli</em>, respectively. The results of the above study indicate FRF as a valuable ingredient for the development of antimicrobial textiles.</p>

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals Agar Diffusion Method for the Assay of Colicins

1968 ◽  
Vol 16 (10) ◽  
pp. 1468-1474 ◽  
Author(s):  
H. Richardson ◽  
A. H. Emslie-Smith ◽  
B. W. Senior
Keyword(s):  
Diffusion Method ◽  
Agar Diffusion ◽  
Agar Diffusion Method

scholarly journals Cytotoxicity tests for nanostructured chitosan/PEO membranes using the agar diffusion method

2012 ◽  
Vol 15 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Valcinir Aloisio Scalla Vulcani ◽  
Maria Trindade Marques Bizarria ◽  
Marcos Akira d’Ávila ◽  
Lucia Helena Innocentini Mei ◽  
Cláudia Bernal ◽  
...  
Keyword(s):  
Diffusion Method ◽  
Agar Diffusion ◽  
Agar Diffusion Method
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