scholarly journals Identification Bacterial Contaminant in Semiarundinaria fastuosa Tissue Culture

2020 ◽  
Vol 3 ◽  
pp. 39-42
Author(s):  
Erna Wulandari ◽  
Endah Retnaningrum ◽  
Langkah Sembiring
Keyword(s):  
Tissue Culture ◽  
Similarity Index ◽  
Culture Technique ◽  
Phenotypic Characterization ◽  
Bacterial Isolation ◽  
Linkage Algorithm ◽  
Bacterial Contaminant ◽  
Average Linkage ◽  
Bacterial Contaminants ◽  
Profile Matching

Bamboo is one of the plants which propagated by tissue culture technique however, the emergence of microbial contaminant caused decrease of bamboo production. A type of microbe that caused the contamination on Semiarundinaria fastuosa tissue culture is bacteria. This study aimed to isolate bacterial contaminant and understanding its biodiversity. Bacterial isolation and phenotypic characterization were done by observing morphology, physiological test, biochemical test, identification and numeric phenetic analysis. Twelve bacterial contaminants was isolated and based on profile matching with Bergey’s Manual of Determinative Bacteriology, six isolate was a member of genus Bacillus, one isolate Enterococcus, two isolate Xenorhabdus, one isolate Morococcus, one isolate Corynebacterium, and one isolate belongs to genus Sarcina. A dendrogram created using Simple Matching coefficient (SSM) parameter and average linkage algorithm shown that on the 71% similarity index, all 12 OTU was grouped in one cluster. However, a dendrogram produced by Jaccard’s coefficient (SJ) parameter and average linkage algorithm on 70% similarity index divided 12 OTU on to 12 clusters. 

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals Production high class of micro tuber potato seeds (Solanum tuberosum L.). by Using tissue culture technique

2009 ◽  
Vol 3 (2) ◽  
pp. 99-106
Author(s):  
A.A. Al-jibouri ◽  
A.A. Al-salhay
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum L ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Condition Numbers ◽  
Mass Propagation ◽  
High Class ◽  
Dormancy Period ◽  
Elisa Technique

The aim of this investigation was produced micro tubers of four potato cultivars Premiere, Bintje, Estima and Escort in vitro. Apical meristems (0.2-0.4 mm) of potato cultivars were excised and cultured on nutrient medium and incubated at 24±2 Cº and 1000 lux light intensity for 16 hrs per day. The developing plantlets were examined serological by using ELISA technique to eliminate the viral infected plantlets. The virus-free plantlets were chopped into pieces with single bud and re cultured on fresh medium for mass propagation. For micro tubers formation in test tubes, the cultures were transferred to another medium containing a high percent of sucrose (60g/L) with different concentrations of kinetin; the cultures were incubated under 16±2 Cº and 8 hrs photoperiod. The plantlets formed micro tubers after 8-10 weeks from culturing. The results showed significant differences among cultivar’s in their response to in vitro culture and micro tubers formation. The results also showed that the kinetin concentration had significant effect on micro tubers, and 1mg/l kinetin concentration was the best. The micro tubers were stored for 10 week at 4Cº to break down the dormancy period, and gave 100% germination under nursery condition. Numbers of tubers derived from micro tubers and normal tubers of these cultivars were compared at the end of season.

scholarly journals Initiation of Ipomoea batatas Through Tissue Culture Technique

2021 ◽  
Vol 12 (2) ◽  
pp. 79-83
Author(s):  
Mahmoud Ali ◽  
Ali Abido ◽  
Mohamed El-Torky ◽  
Badria Hassan ◽  
Marwa Abdelwahab
Keyword(s):  
Tissue Culture ◽  
Ipomoea Batatas ◽  
Culture Technique ◽  
Tissue Culture Technique

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

scholarly journals PRIMING OF GENETIC VARIATIONS BY COLOCYNTH FRUIT EXTRACT AND CALLUS INITIATION IN EMBRYOS OF TWO CULTIVARS OF ALFALFA SEEDS

2017 ◽  
Vol 48 (3) ◽  
Author(s):  
Hamza & Ali
Keyword(s):  
Tissue Culture ◽  
Dry Weight ◽  
Culture Technique ◽  
Factorial Experiment ◽  
Graduate Studies ◽  
College Of Agriculture ◽  
Fruit Extract ◽  
Local Variety ◽  
Molecular Weights ◽  
Callus Initiation

An experiment  was conducted at Central lab of Graduate studies, College of Agriculture, University of Baghdad during 2015-2016. The aim was to study the effect of colocynth fruit extract on inducing callus from embryos of two alfalfa cultivars were PAC-78001 and local variety by tissue culture technique using factorial experiment within CRD. Seeds was soaked with colocynth fruit extract at 0, 75, 150, 225, and 300 ml Li-1 for 24 hours, then, sterilized by NaOCl at 4.5% for 15 minutes. The results showed significant differences between two cultivars responding to colocynth fruit extract levels. Concentration at 150 ml Li-1 gave the highest both fresh and dry weight of callus (205.90 and 20.60) respectively. Also, PCR results depending on RAPD and electrophoresis for DNA samples which isolated from callus of two cultivars and subjected to various concentrations of colocynth fruit extract 0, 75, 150, 225 and 300 ml Li-1 showed differences in amplified observed bands, molecular weights and brightness intensity by using primers primer -Y07, -Y06, OE-12, C-08, D-10, -Y10 and A-16, and There was no difference in bands number at all concentrations of colocynth fruit extract for local variety by primer A-16.       

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