scholarly journals Histological Study of Rete Testis and Ductuli efferentes of Rabbit (Oryctolagus cuniculus)

2019 ◽  
Vol 2 ◽  
pp. 145-147
Author(s):  
Rakhmiyati Rakhmiyati ◽  
Muhammad Jafar Luthfi
Keyword(s):  
Histological Study ◽  
Rete Testis ◽  
Seminiferous Tubules ◽  
Sperm Cells ◽  
Observation Data ◽  
Thin Walls ◽  
Complex Part ◽  
Ductuli Efferentes ◽  
Pass Through ◽  
Testis Tissue

The testes produce sperm cells through the spermatogenesis process that occurs in the complex system ducts starting form seminiferous tubules to vas deferens. Testis has a very complex part to support sperm cell production. Sperm cells that have been formed through the process of spermatogenesis will move towards the lumen in the seminiferous tubules. Then the sperm cells will pass through the rete testis tissue, then to ductuli efferentes to arrive at the epididymis for the process of maturing sperm. Anatomical studies on seminiferous tubular tissue and epididymal organs have often been carried out, but there is still very little observation data on rete testis and ductuli efferentes in rabbits. The method used in this study was the paraffin method with hematoxylin-eosin (HE) staining. The observations showed that the structure of the testicular rete channels had thin walls compared to the seminifous tubule channels. Ductuli efferens is a channel between of rete testis and epididymis.

scholarly journals PSXIII-5 The age dynamics of the spermatogenic cells development in the roosters’ testes

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 373-373
Author(s):  
Anastasia N Vetokh ◽  
Natalia A Volkova ◽  
Evgeniya K Tomgorova ◽  
Ludmila A Volkova ◽  
Natalia A Zinovieva
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Genetic Material ◽  
Cell Types ◽  
Seminiferous Tubules ◽  
Sperm Cells ◽  
Spermatogenic Cells ◽  
Different Cell Types ◽  
Hematoxylin Eosin ◽  
Testis Tissue

Abstract The cells of the male gonads are considered as a valuable genetic material for the conservation of the gene pool of breeds and lines of agricultural birds, as well as the directed modification of the poultry genome. Mature germ cells – spermatozoa and their predecessors – spermatogonia, spermatocytes and spermatids can be used for these purposes. To obtain these types of cells, it is necessary to know the characteristics of their development (spermatogenesis). The dynamics of the development of certain spermatogenic cell types in the testicular tubules of different-aged roosters has been studied. Histological studies were performed on testes of roosters aged from 1 week to 6 months with an interval of 2 weeks. Samples of testis tissue were fixed in Bouin’s solution. Histological sections were stained with hematoxylin-eosin. Identification of different cell types (Sertoli, spermatogonia, spermatocytes, spermatids, sperm cells) was carried out according to their morphology. At the age of 1–6 weeks in the seminiferous tubule of roosters, the mainly presence of two cell types was noted: Sertoli cells and spermatogonia. From 7 weeks of age, spermatocytes were detected in the seminiferous tubules, in the 4 months - spermatids, in the 5.5 months - sperm cells. The number of Sertoli cells remained almost unchanged with age and was 21 ± 2. The percentage of these cells decreased with age from 71 ± 3 % to 5 ± 1 %. The percentage of spermatogonia also decreased with age from 75 ± 2 % to 7 ± 1 %. The number of spermatids and spermatozoa, on the contrary, increased to puberty (6 months) and reached 54 %. The study was supported by the RFBR within Project no.18-29-07079.

scholarly journals Induction of meiosis in fetal mouse testis in vitro by rete testis tissue from pubertal mice and bulls

Reproduction ◽  
1979 ◽  
Vol 56 (2) ◽  
pp. 653-656 ◽  
Author(s):  
J. Grinsted ◽  
A. G. Byskov ◽  
M. P. Andreasen
Keyword(s):  
Rete Testis ◽  
Mouse Testis ◽  
Fetal Mouse ◽  
Testis Tissue

scholarly journals Histological study of the effect of paracetamol on the seminiferous tubules of adult rabbits

2011 ◽  
Vol 34 (4) ◽  
pp. 790-799
Author(s):  
Mohamed M. Yousef ◽  
Omayma K. Helal ◽  
Nermeen Adly
Keyword(s):  
Histological Study ◽  
Seminiferous Tubules

The Structure of the Male Genital System of the Port Jackson Shark, Heterodontus portujacksoni, with Particular reference to the Genital Ducts

1982 ◽  
Vol 30 (4) ◽  
pp. 523 ◽  
Author(s):  
N Jones ◽  
RC Jones
Keyword(s):  
Sertoli Cell ◽  
Initial Segment ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Terminal Segment ◽  
Rete Testis ◽  
Ductuli Efferentes ◽  
Pseudostratified Epithelium ◽  
Port Jackson ◽  
Germinal Cells

The testes of H. portusjacksoni are paired cylindrical organs with a germinal ridge running longitudinally along the ventral surface. The latter produces ampullae which contain Sertoli and germinal cells, and which migrate dorsally as spermatogenesis proceeds. They evacuate near the dorsal surface of the testis to release spermatozoa, large oval eosinophilic bodies (Sertoli cell bodies) and small eosinophilic particles. All of the genital ducts are lined by a ciliated epithelium. In the rete testis it is a low columnar type; the ducts consist of the conducting tubules, which convey ampullae across the testis, and the longitudinal testis canal, which runs along the dorsal surface of the testis. Six ductuli efferentes (lined by a low pseudostratified columnar epithelium) leave the testis, course dorsally through the epigonal organ and mesorchium, and enter the epididymis. Subsequently, pairs of ductuli efferentes successively join together to form a short common ductulus efferent which then continues as the ductus epididymidis. The latter has two structurally distinct regions: the initial segment is a narrow, convoluted, pigmented duct with a very tall pseudostratified epithelium; the terminal segment is wider than the initial segment, unpigmented and lined by a low pseudostratified epithelium. It is very convoluted proximally, but widens and becomes less convoluted distally; its distal 5 cm (ampulla of ductus epididymidis) is straight and contains a muscular wall. Very few Sertoli cell bodies are present in the terminal segment of the ductus epididymidis. However, except for the ampullary region the duct receives secretions (including irregularly shaped eosinophilic bodies) from Leydig's gland (a series of branched tubular glands). Secretions from these glands seem to be involved in the formation of spermatozoa into spherical bundles. The structure of the clasper and siphon are assessed in terms of their function and significance for taxonomical purposes.

scholarly journals Germ cell survival and differentiation after xenotransplantation of testis tissue from three endangered species: Iberian lynx (Lynx pardinus), Cuvier's gazelle (Gazella cuvieri) and Mohor gazelle (G. dama mhorr)

2014 ◽  
Vol 26 (6) ◽  
pp. 817 ◽  
Author(s):  
Lucía Arregui ◽  
Ina Dobrinski ◽  
Eduardo R. S. Roldan
Keyword(s):  
Endangered Species ◽  
Biological Diversity ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Lynx Lynx ◽  
Lynx Pardinus ◽  
Reproductive Techniques ◽  
First Time ◽  
Potential Use ◽  
Testis Tissue

The use of assisted reproductive techniques for endangered species is a major goal for conservation. One of these techniques, testis tissue xenografting, allows for the development of spermatozoa from animals that die before reaching sexual maturity. To assess the potential use of this technique with endangered species, testis tissue from six Iberian lynxes (one fetus, two perinatal cubs, two 6-month-old and one 2-year-old lynx), two Cuvier’s gazelle fetuses and one 8-month-old Mohor gazelle were transplanted ectopically into nude mice. Tissue from the lynx fetus, perinatal cubs and 2-year-old donors degenerated, whereas spermatogonia were present in 15% of seminiferous tubules more than 70 weeks after grafting in transplanted testis tissue from 6-month-old donors. Seminal vesicle weights (indicative of testosterone production) increased over time in mice transplanted with tissue from 6-month-old lynxes. Progression of spermatogenesis was observed in xenografts from gazelles and was donor age dependent. Tissue from Cuvier’s gazelle fetuses contained spermatocytes 40 weeks after grafting. Finally, round spermatids were found 28 weeks after transplantation in grafts from the 8-month-old Mohor gazelle. This is the first time that xenotransplantation of testicular tissue has been performed with an endangered felid and the first successful xenotransplantation in an endangered species. Our results open important options for the preservation of biological diversity.

Development of the seminiferous tubules and rete testis during prenatal human development

1978 ◽  
Vol 85 (2) ◽  
pp. 233-235
Author(s):  
O. I. Brindak ◽  
V. A. Vlasov ◽  
V. A. Malishevskaya
Keyword(s):  
Human Development ◽  
Rete Testis ◽  
Seminiferous Tubules

Evaluation of the reproductive toxicity of naproxen sodium and meloxicam in male rats

2014 ◽  
Vol 34 (4) ◽  
pp. 415-429 ◽  
Author(s):  
B Uzun ◽  
O Atli ◽  
BO Perk ◽  
D Burukoglu ◽  
S Ilgin
Keyword(s):  
Naproxen Sodium ◽  
Histopathological Examination ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Prostaglandin E ◽  
Cox 2 ◽  
Cox 1 ◽  
Testis Tissue

Nonsteroidal anti-inflammatory drugs that are cyclooxygenase (COX) enzyme inhibitors have generally been used in short-term pain management and also to treat inflammation chronically. It is known that COX enzyme and prostaglandins play important roles in the regulation of reproductive functions in females. However, there are relatively few studies for the male reproductive system, and the results of these studies are contradictory. In this study, sperm count and motility, COX-1, COX-2, prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), and prostaglandin F2α (PGF2α) levels in testis tissue, plasma follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels, and histopathological examination of testis tissue were evaluated after naproxen sodium and meloxicam administration in male rats. Also, testis superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione (GSH) levels were measured to investigate the oxidation status. According to our results, sperm count and motility were significantly decreased in treatment groups. Plasma hormone levels did not show any statistical differences between the groups. COX-1, PGE2, and PGF2α levels were significantly decreased, while the decreases in COX-2 and PGE1 levels did not show any significance statistically. Testis SOD, catalase, GPx, and GSH levels were decreased significantly. According to the results of histopathological examination, damage in seminiferous tubules, where spermatogenesis developed, was observed. In conclusion, naproxen sodium and meloxicam decreased the sperm count and motility and also induced the damage of seminiferous tubules as a direct effect without affecting plasma hormone levels in our study. The mechanism of the reproductive toxicity induced by these agents may be based on the inhibition of prostaglandin synthesis and the induction of oxidative stress can be emphasized as a secondary factor.

Male germ cell transplantation: promise and problems

2001 ◽  
Vol 13 (8) ◽  
pp. 609 ◽  
Author(s):  
Fang-Xu Jiang
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Cells ◽  
Cell Biology ◽  
Direct Injection ◽  
Primordial Germ Cells ◽  
Rete Testis ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Germ Cell Transplantation

Male germ cell transplantation is a novel technique in which donor male stem germ cells are surgically transferred to the seminiferous tubules of a recipient testis by direct injection or via the rete testis or efferent duct. All germ cells that are destined to become stem spermatogonia are defined as male stem germ cells, including primordial germ cells from the gonadal ridges, and gonocytes and stem spermatogonia from the testis, all of which are transplantable and capable of undergoing normal spermatogenesis. Xenotransplantation of male germ cells from one species into the testis of another species, including human testicular cells in the mouse, has so far proved to be unsuccessful. However, the immunodeficient mouse testis can support rat spermatogenesis and produce apparently normal rat spermatozoa. The underlying mechanisms remain elusive. The present mini-review will focus on the importance of stem spermatogonial transplantation for testicular stem cell biology and discuss the likelihood of immune rejection after transplantation, which may limit the success of all male germ cell transplantation.

OESTRADIOL-17β AND TESTOSTERONE IN RAT TESTIS TISSUE: EFFECT OF GONADO-TROPHINS, LOCALIZATION AND PRODUCTION IN VITRO

1974 ◽  
Vol 60 (3) ◽  
pp. 409-419 ◽  
Author(s):  
F. H. de JONG ◽  
A. H. HEY ◽  
H. J. van der MOLEN
Keyword(s):  
Intravenous Injection ◽  
Testicular Tissue ◽  
Prolonged Treatment ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Tissue Concentrations ◽  
Hypophysectomized Rats ◽  
Trophic Hormone ◽  
Testis Tissue

SUMMARY Concentrations of oestradiol-17β and testosterone were estimated in testicular tissue from intact and hypophysectomized rats. Within 30 min after intravenous injection of human chorionic gonadotrophin (HCG) or follicle-stimulating hormone (FSH) to intact animals the tissue concentrations of both steroids were not significantly changed. Prolonged s.c. administration of HCG (5 days) caused an increase in the tissue levels of both steroids, which was further increased when the prolonged treatment was followed by an intravenous injection with this trophic hormone. FSH had no influence on tissue concentrations of oestradiol-17β or testosterone in hypophysectomized rats. Assay of separated seminiferous tubules and interstitial tissue indicated that oestradiol-17β and testosterone were mainly localized in the interstitial tissue. Incubations of these constituents showed that oestradiol-17β was produced in the seminiferous tubules, while testosterone was produced in the interstitial compartment.

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