scholarly journals Long-Term Differentiation of Human Embryonic Stem Cell-Derived Neural Progenitors (hESNPs) into Inhibitory Interneurons In Vitro

2018 ◽  
Author(s):  
Trinithas J. Boyi
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
Inhibitory Interneurons

Transcriptome and in Vitro Differentiation Profile of Human Embryonic Stem Cell Derived NKX2.1-Positive Neural Progenitors

2016 ◽  
Vol 12 (6) ◽  
pp. 744-756 ◽  
Author(s):  
Christopher Y. Chen ◽  
Alex Plocik ◽  
Nickesha C. Anderson ◽  
Daniel Moakley ◽  
Trinithas Boyi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
In Vitro Differentiation

Interactions of Human Embryonic Stem Cell-Derived Neural Progenitors with an Electrospun Nanofibrillar Surface in Vitro

2011 ◽  
Vol 34 (7) ◽  
pp. 559-570 ◽  
Author(s):  
Ali Rahjouei ◽  
Sahar Kiani ◽  
Azadeh Zahabi ◽  
Narges Zare Mehrjardi ◽  
Mehrdad Hashemi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Neural Progenitors

In vitro differentiation of retinal ganglion-like cells from embryonic stem cell derived neural progenitors

2009 ◽  
Vol 380 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Balusamy Jagatha ◽  
Mundackal S. Divya ◽  
Rajendran Sanalkumar ◽  
Chandrasekharan L. Indulekha ◽  
Sasidharan Vidyanand ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
Retinal Ganglion ◽  
In Vitro Differentiation

scholarly journals In vitro and in vivo analyses of human embryonic stem cell-derived dopamine neurons

2005 ◽  
Vol 92 (5) ◽  
pp. 1265-1276 ◽  
Author(s):  
Chang-Hwan Park ◽  
Yang-Ki Minn ◽  
Ji-Yeon Lee ◽  
Dong Ho Choi ◽  
Mi-Yoon Chang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Dopamine Neurons

Assessment of the ultrastructural and proliferative properties of human embryonic stem cell-derived cardiomyocytes

2003 ◽  
Vol 285 (6) ◽  
pp. H2355-H2363 ◽  
Author(s):  
Mirit Snir ◽  
Izhak Kehat ◽  
Amira Gepstein ◽  
Raymond Coleman ◽  
Joseph Itskovitz-Eldor ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Late Stage ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Es Cell ◽  
Cardiomyocyte Proliferation ◽  
Ki 67

Assessment of early ultrastructural development and cell-cycle regulation in human cardiac tissue is significantly hampered by the lack of a suitable in vitro model. Here we describe the possible utilization of human embryonic stem cell (ES) lines for investigation of these processes. With the use of the embryoid body (EB) differentiation system, human ES cell-derived cardiomyocytes at different developmental stages were isolated and their histomorphometric, ultrastructural, and proliferative properties were characterized. Histomorphometric analysis revealed an increase in cell length, area, and length-to-width ratio in late-stage EBs (>35 days) compared with early (10–21 days) and intermediate (21–35 days) stages. This was coupled with a progressive ultrastructural development from an irregular myofibrillar distribution to an organized sarcomeric pattern. Cardiomyocyte proliferation, assessed by double labeling with cardiac-specific antibodies and either [3H]thymidine incorporation or Ki-67 immunolabeling, demonstrated a gradual withdrawal from cell cycle. Hence, the percentage of positively stained nuclei in early-stage cardiomyocytes ([3H]thymidine: 60 ± 10%, Ki-67: 54 ± 23%) decreased to 36 ± 7% and 9 ± 16% in intermediate-stage EBs and to <1% in late-stage cardiomyocytes. In conclusion, a reproducible temporal pattern of early cardiomyocyte proliferation, cell-cycle withdrawal, and ultrastructural maturation was noted in this model. Establishment of this unique in vitro surrogate system may allow to examine the molecular mechanisms underlying these processes and to assess interventions aiming to modify these properties. Moreover, the detailed characterization of the ES cell-derived cardiomyocyte may be crucial for the development of future cell replacement strategies aiming to regenerate functional myocardium.

scholarly journals Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52214 ◽  
Author(s):  
Andrew B. J. Prowse ◽  
Fenny Chong ◽  
David A. Elliott ◽  
Andrew G. Elefanty ◽  
Edouard G. Stanley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Mitochondrial Function ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation

In Vitro Electrophysiological Drug Testing Using Human Embryonic Stem Cell Derived Cardiomyocytes

2009 ◽  
Vol 18 (1) ◽  
pp. 161-172 ◽  
Author(s):  
Oren Caspi ◽  
Ilanit Itzhaki ◽  
Izhak Kehat ◽  
Amira Gepstein ◽  
Gil Arbel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Drug Testing ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

scholarly journals In vitro human embryonic stem cell hematopoiesis mimics MYB-independent yolk sac hematopoiesis

Haematologica ◽  
2014 ◽  
Vol 100 (2) ◽  
pp. 157-166 ◽  
Author(s):  
S. Vanhee ◽  
K. De Mulder ◽  
Y. Van Caeneghem ◽  
G. Verstichel ◽  
N. Van Roy ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Yolk Sac
Sign in / Sign up

Export Citation Format

Share Document