scholarly journals Dispersal and molecular characterisation of the Echinococcus granulosus (Batsch, 1786) complex isolated from various intermediate hosts in the Calabria region, southern Italy

2020 ◽  
Vol 67 ◽  
Author(s):  
Grazia Pavia ◽  
Federica De Gori ◽  
Lucia Ciambrone ◽  
Natalino De Gori ◽  
Rosanna Musarella ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Southern Italy ◽  
Molecular Characterisation ◽  
Intermediate Hosts ◽  
Calabria Region

scholarly journals Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus

2018 ◽  
Vol 19 (9) ◽  
pp. 2669 ◽  
Author(s):  
Ning Wang ◽  
Jiafei Zhan ◽  
Cheng Guo ◽  
Chunyan Li ◽  
Nengxing Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Echinococcus Granulosus ◽  
Mitochondrial Fission ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Economic Losses ◽  
Molecular Characterisation ◽  
Intermediate Hosts ◽  
H2o2 Treatment ◽  
Induced Apoptosis ◽  
Cell Death Protein

Cystic echinococcosis, a parasitic zoonosis that causes significant economic losses and poses a threat to public health, is caused by larvae of the tapeworm Echinococcus granulosus. Infection causes infertile cysts in intermediate hosts that cannot produce protoscoleces (PSCs) or complete the life cycle. Herein, we cloned, expressed, and characterised mitochondrial fission protein 1 (Eg-Fis1) and programmed cell death protein 6 (Eg-PDCD6) from E. granulosus, and explored their functions related to infertile cysts. Eg-Fis1 and Eg-PDCD6 encode putative 157 and 174 residue proteins, respectively, and Western blotting indicated good reactogenicity for both. Eg-Fis1 and Eg-PDCD6 were ubiquitously distributed in all stages of E. granulosus. Furthermore, mRNAs of Eg-Fis1 and Eg-PDCD6 were upregulated following H2O2 treatment which induced apoptosis in PSCs. To investigate the regulation of apoptosis in response to oxidative stress, RNA interference (RNAi) and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assays were performed. The apoptotic rate of the Eg-Fis1 RNAi group was significantly lower than non-interference group, but there was no such difference for Eg-PDCD6. In conclusion, Eg-Fis1 promotes apoptosis induced by oxidative stress, whereas Eg-PDCD6 does not appear to be a key regulator of apoptosis.

Assessing Seismic Resilience of School Educational Sector. An attempt to establish the initial conditions in Calabria Region, Southern Italy

2021 ◽  
Author(s):  
Cora Fontana ◽  
Eleonora Cianci ◽  
Massimiliano Moscatelli
Keyword(s):  
Urban Areas ◽  
Southern Italy ◽  
Initial Conditions ◽  
Risk Mitigation ◽  
School Buildings ◽  
Calabria Region ◽  
Structural Improvement ◽  
First Case ◽  
School Sector ◽  
Seismic Resilience

<p>School education constitutes one of the strategic functions to be recovered after an earthquake. The structural improvement of school buildings together with the strengthening of the administrators’ capacity to react positively following an earthquake are key factors that contribute to social vulnerability’s reduction. Nevertheless, in Italy, the issue of risk reduction policies related to school sector is not yet consolidated in the institutional agendas. Observing the last major Italian earthquakes what remains predominant is school buildings’ damage degree with consequent interruption of the system functionality. Among the causes: the building heritage vulnerability and the lack of risk mitigation policies, capable of building a resilient community for future earthquakes. That of resilience is considered a relevant paradigm to address the issue of how to strengthen the school sector’s capacity to ensure the buildings physical safety and to guarantee the maintenance of the school function, looking at pre and post-event phases.</p><p>The paper proposes a set of indicators and a methodology for a preliminary assessment of the educational sector’s seismic resilience, in terms of initial conditions. The method has been tested on a first case study: Calabria Region, Southern Italy. The results show that spatial differences in the educational sector’s seismic resilience are evident. Except for some large urban areas, the less resilient areas are grouped mainly in the southern part of the Region, while the most resilient ones are located mostly in the central-northern sector. The ambition is to identify a repeatable approach, useful as guidelines for school seismic prevention policies.</p>

scholarly journals Cystic Echinococcosis: Clinical, Immunological, and Biomolecular Evaluation of Patients from Sardinia (Italy)

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 907
Author(s):  
Cinzia Santucciu ◽  
Piero Bonelli ◽  
Angela Peruzzu ◽  
Alessandro Fancellu ◽  
Vincenzo Marras ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Public Health Problem ◽  
Sensu Stricto ◽  
Surgical Removal ◽  
Neighbor Joining ◽  
Intermediate Hosts ◽  
High Prevalence ◽  
Echinococcus Granulosus Sensu Lato

Cystic echinococcosis (CE), a zoonotic disease caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s.l.), is a worldwide public health problem. Echinococcus granulosus sensu stricto (s.s.), associated with G1 and G3 genotypes, is endemic with high prevalence in the Mediterranean basin. The parasite’s life cycle comprises definitive hosts (canids) and intermediate hosts (ruminants) and can occasionally involve humans. The main aim of this research was to confirm the diagnosis of 13 patients suspected of CE who presented different complications and needed the surgical removal of the cysts. We also wanted to understand and clarify more the diagnosis of echinococcosis in humans. For this purpose, the patients first underwent cyst evaluation by ultrasound (US), immunological analysis, and then total pericystectomy, followed by parasitological, histopathological, and molecular biology examinations of the cysts. US stadiated one CE1, one CE2, eight CE3b, one CE4, and two CE5; immunology evidenced nine positives; histopathology confirmed 11 CE cysts, of which 8 fertile presenting protoscoleces were identified as E. granulosus s.s. by molecular biology, genotyped as three G1 and four G3 by neighbor-joining (NJ) phylogenetic tree. In conclusion, the results showed that 11 patients were affected by E. granulosus s.s. G1 orG3, and 2 cystic neoformations were of non-parasitic origin.

Echinococcus granulosus: genomic and isoenzymatic study of Spanish strains isolated from different intermediate hosts

1996 ◽  
Vol 63 (3-4) ◽  
pp. 273-282 ◽  
Author(s):  
Mar Siles-Lucas ◽  
Mario C. Benito ◽  
Carmen Cuesta-Bandera
Keyword(s):  
Echinococcus Granulosus ◽  
Intermediate Hosts

Observations on the suitability and importance of the domestic intermediate hosts of Echinococcus granulosus in Uttah Pradesh, India

1989 ◽  
Vol 63 (1) ◽  
pp. 39-45 ◽  
Author(s):  
M. Irshadullah ◽  
W. A. Nizami ◽  
C. N. L. Macpherson
Keyword(s):  
Life Cycle ◽  
Intermediate Host ◽  
Echinococcus Granulosus ◽  
Frequency Distribution ◽  
Host Species ◽  
Control Measures ◽  
The Other ◽  
Hydatid Cysts ◽  
Intermediate Hosts ◽  
Cyst Size

ABSTRACTThe present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffaloes slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.

scholarly journals Molecular Characterization of Echinococcus granulosus sensu lato from Humans in Slovenia

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 562
Author(s):  
Barbara Šoba ◽  
Špela Gašperšič ◽  
Darja Keše ◽  
Tadeja Kotar
Keyword(s):  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Nadh Dehydrogenase ◽  
Sensu Stricto ◽  
Intermediate Hosts ◽  
Western Balkans ◽  
Nadh Dehydrogenase Subunit ◽  
Important Intermediate ◽  
Echinococcus Granulosus Sensu Lato

The larval form of tapeworms of the Echinococcus granulosus sensu lato species cluster cause an important zoonotic infection, cystic echinococcosis (CE). Molecular characterization of the cluster’s isolates from different hosts greatly contributes to a better understanding of its transmission dynamics. To date, no genetic information is available on CE in Slovenia. In this work, we characterized isolates from human CE cases. Parasite samples from 18 patients were collected, together with the patients’ demographic and clinical data. Genomic DNA was analyzed by conventional PCR and sequencing at four mitochondrial loci (cytochrome c oxidase subunit 1, cox1; NADH dehydrogenase subunit 1, nad1; NADH dehydrogenase subunit 5, nad5; and small ribosomal RNA, rrnS). Thirteen isolates were successfully amplified and sequenced. Seven (58.8%) patients were infected with E. granulosus sensu stricto (s.s.) G1, five (38.5%) with E. canadensis G7 and one (7.7%) with E. granulosus s.s. G3. Echinococcus canadensis G7, the pig genotype, was identified exclusively in autochthonous Slovenes, while the patients originating from the Western Balkans were all infected with E. granulosus s.s. Our findings suggest that pigs are important intermediate hosts for human CE in Slovenia.

Morphological and molecular characterisation of Echinococcus granulosus from goat isolates in Iran

Acta Tropica ◽  
2012 ◽  
Vol 123 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Mohammad Rajabloo ◽  
Seyed Hossein Hosseini ◽  
Fatemeh Jalousian
Keyword(s):  
Echinococcus Granulosus ◽  
Molecular Characterisation

Canine echinococcosis: the predominance of immature eggs in adult tapeworms of Echinococcus granulosus in stray dogs from Tunisia

2016 ◽  
Vol 91 (3) ◽  
pp. 380-383 ◽  
Author(s):  
W. Iraqi
Keyword(s):  
Public Health ◽  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Health Problem ◽  
Small Proportion ◽  
Public Health Problem ◽  
Hydatid Cysts ◽  
Intermediate Hosts ◽  
Stray Dogs ◽  
Adult Tapeworm

AbstractCanine echinococcosis is caused by the adult tapeworm of Echinococcus granulosus. As intermediate hosts, humans and livestock become infected following ingestion of eggs that are passed in the faeces of dogs. Mature eggs develop into hydatid cysts in different organs, leading to hydatid disease, which is a serious public health problem. In the present study, we investigated the proportion of mature eggs of E. granulosus in 140 dogs from three regions of Tunisia. The results showed the predominance of immature E. granulosus eggs in infected dogs and the occurrence of a small proportion of oncospheres. The ability of immature eggs to infect humans and livestock is discussed.

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